Slit1 and Slit2 cooperate to prevent premature midline crossing of retinal axons in the mouse visual system

A. S. Plump, Lynda Erskine, C. Sabatier, K. Brose, C. J. Epstein, C. S. Goodman, C. A. Mason, M. Tessier-Lavigne

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Abstract

During development, retinal ganglion cell (RGC) axons either cross or avoid the midline at the optic chiasm. In Drosophila, the Slit protein regulates midline axon crossing through repulsion. To determine the role of Slit proteins in RGC axon guidance, we disrupted Slit1 and Slit2, two of three known mouse Slit genes. Mice defective in either gene alone exhibited few RGC axon guidance defects, but in double mutant mice a large additional chiasm developed anterior to the true chiasm, many retinal axons projected into the contralateral optic nerve, and some extended ectopically-dorsal and lateral to the chiasm. Our results indicate that Slit proteins repel retinal axons in vivo and cooperate to establish a corridor through which the axons are channeled, thereby helping define the site in the ventral diencephalon where the optic chiasm forms.

Original languageEnglish
Pages (from-to)219-232
Number of pages13
JournalNeuron
Volume33
Issue number2
DOIs
Publication statusPublished - 17 Jan 2002

Keywords

  • ganglion-cell axons
  • growth cone behavior
  • optic chiasm
  • chondrotin sulfate
  • neuronal migration
  • uncrossed axons
  • ephrina ligands
  • vertebrate slit
  • nervous-system
  • guidance

Cite this

Plump, A. S., Erskine, L., Sabatier, C., Brose, K., Epstein, C. J., Goodman, C. S., Mason, C. A., & Tessier-Lavigne, M. (2002). Slit1 and Slit2 cooperate to prevent premature midline crossing of retinal axons in the mouse visual system. Neuron, 33(2), 219-232. https://doi.org/10.1016/S0896-6273(01)00586-4