Slits contribute to the guidance of retinal ganglion cell axons in the mammalian optic tract

Hannah Thompson, David Barker, Olivier Camand, Lynda Erskine

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

RGC axons extend in the optic tracts in a manner that correlates with the expression in the hypothalamus and epithalamus of a soluble factor inhibitory to RGC axon outgrowth. Additionally, although the RGC axons extend adjacent to the telencephalon, they do not normally grow into this tissue. Here, we show that slit1 and slit2, known chemorepellents for RGC axons expressed in specific regions of the diencephalon and telencephalon, help regulate optic tract development. In mice lacking slit1 and slit2, a subset of RGC axons extend into the telencephalon and grow along the pial surface but not more deeply into this tissue. Surprisingly, distinct guidance errors occur in the telencephalon of slit1 -/-; slit2 +/- and slit1/2 -/- embryos, suggesting that the precise level of Slits is critical for determining the path followed by individual axons. In mice lacking both slit1 and slit2, a subset of RGC axons also project aberrantly into the epithalamus, pineal and across the dorsal midline. However, many axons reach their primary target, the superior colliculus. This demonstrates that Slits play an important role in directing the guidance of post-crossing RGC axons within the optic tracts but are not required for target innervation.
Original languageEnglish
Pages (from-to)476-484
Number of pages9
JournalDevelopmental Biology
Volume296
Issue number2
Early online date14 Jun 2006
DOIs
Publication statusPublished - 15 Aug 2006

Fingerprint

Retinal Ganglion Cells
Axons
Telencephalon
Epithalamus
Diencephalon
Critical Pathways
Optic Tract
Superior Colliculi
Hypothalamus
Embryonic Structures

Keywords

  • Animals
  • Axons
  • Female
  • Intercellular Signaling Peptides and Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Nerve Tissue Proteins
  • Retinal Ganglion Cells
  • Visual Pathways
  • Axon guidance
  • Slit
  • Robo
  • Optic tract
  • Retinal ganglion cell
  • Growth cone

Cite this

Slits contribute to the guidance of retinal ganglion cell axons in the mammalian optic tract. / Thompson, Hannah; Barker, David; Camand, Olivier; Erskine, Lynda.

In: Developmental Biology, Vol. 296, No. 2, 15.08.2006, p. 476-484.

Research output: Contribution to journalArticle

Thompson, Hannah ; Barker, David ; Camand, Olivier ; Erskine, Lynda. / Slits contribute to the guidance of retinal ganglion cell axons in the mammalian optic tract. In: Developmental Biology. 2006 ; Vol. 296, No. 2. pp. 476-484.
@article{0160a87eef7344ceb686ce31ff92a136,
title = "Slits contribute to the guidance of retinal ganglion cell axons in the mammalian optic tract",
abstract = "RGC axons extend in the optic tracts in a manner that correlates with the expression in the hypothalamus and epithalamus of a soluble factor inhibitory to RGC axon outgrowth. Additionally, although the RGC axons extend adjacent to the telencephalon, they do not normally grow into this tissue. Here, we show that slit1 and slit2, known chemorepellents for RGC axons expressed in specific regions of the diencephalon and telencephalon, help regulate optic tract development. In mice lacking slit1 and slit2, a subset of RGC axons extend into the telencephalon and grow along the pial surface but not more deeply into this tissue. Surprisingly, distinct guidance errors occur in the telencephalon of slit1 -/-; slit2 +/- and slit1/2 -/- embryos, suggesting that the precise level of Slits is critical for determining the path followed by individual axons. In mice lacking both slit1 and slit2, a subset of RGC axons also project aberrantly into the epithalamus, pineal and across the dorsal midline. However, many axons reach their primary target, the superior colliculus. This demonstrates that Slits play an important role in directing the guidance of post-crossing RGC axons within the optic tracts but are not required for target innervation.",
keywords = "Animals, Axons, Female, Intercellular Signaling Peptides and Proteins, Mice, Mice, Inbred C57BL, Mice, Knockout, Nerve Tissue Proteins, Retinal Ganglion Cells, Visual Pathways, Axon guidance, Slit, Robo, Optic tract, Retinal ganglion cell, Growth cone",
author = "Hannah Thompson and David Barker and Olivier Camand and Lynda Erskine",
year = "2006",
month = "8",
day = "15",
doi = "10.1016/j.ydbio.2006.06.017",
language = "English",
volume = "296",
pages = "476--484",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Slits contribute to the guidance of retinal ganglion cell axons in the mammalian optic tract

AU - Thompson, Hannah

AU - Barker, David

AU - Camand, Olivier

AU - Erskine, Lynda

PY - 2006/8/15

Y1 - 2006/8/15

N2 - RGC axons extend in the optic tracts in a manner that correlates with the expression in the hypothalamus and epithalamus of a soluble factor inhibitory to RGC axon outgrowth. Additionally, although the RGC axons extend adjacent to the telencephalon, they do not normally grow into this tissue. Here, we show that slit1 and slit2, known chemorepellents for RGC axons expressed in specific regions of the diencephalon and telencephalon, help regulate optic tract development. In mice lacking slit1 and slit2, a subset of RGC axons extend into the telencephalon and grow along the pial surface but not more deeply into this tissue. Surprisingly, distinct guidance errors occur in the telencephalon of slit1 -/-; slit2 +/- and slit1/2 -/- embryos, suggesting that the precise level of Slits is critical for determining the path followed by individual axons. In mice lacking both slit1 and slit2, a subset of RGC axons also project aberrantly into the epithalamus, pineal and across the dorsal midline. However, many axons reach their primary target, the superior colliculus. This demonstrates that Slits play an important role in directing the guidance of post-crossing RGC axons within the optic tracts but are not required for target innervation.

AB - RGC axons extend in the optic tracts in a manner that correlates with the expression in the hypothalamus and epithalamus of a soluble factor inhibitory to RGC axon outgrowth. Additionally, although the RGC axons extend adjacent to the telencephalon, they do not normally grow into this tissue. Here, we show that slit1 and slit2, known chemorepellents for RGC axons expressed in specific regions of the diencephalon and telencephalon, help regulate optic tract development. In mice lacking slit1 and slit2, a subset of RGC axons extend into the telencephalon and grow along the pial surface but not more deeply into this tissue. Surprisingly, distinct guidance errors occur in the telencephalon of slit1 -/-; slit2 +/- and slit1/2 -/- embryos, suggesting that the precise level of Slits is critical for determining the path followed by individual axons. In mice lacking both slit1 and slit2, a subset of RGC axons also project aberrantly into the epithalamus, pineal and across the dorsal midline. However, many axons reach their primary target, the superior colliculus. This demonstrates that Slits play an important role in directing the guidance of post-crossing RGC axons within the optic tracts but are not required for target innervation.

KW - Animals

KW - Axons

KW - Female

KW - Intercellular Signaling Peptides and Proteins

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Nerve Tissue Proteins

KW - Retinal Ganglion Cells

KW - Visual Pathways

KW - Axon guidance

KW - Slit

KW - Robo

KW - Optic tract

KW - Retinal ganglion cell

KW - Growth cone

U2 - 10.1016/j.ydbio.2006.06.017

DO - 10.1016/j.ydbio.2006.06.017

M3 - Article

C2 - 16828733

VL - 296

SP - 476

EP - 484

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -