Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina

Rivka A Rachel, Gul Dolen, Nancy L Hayes, Alice Lu, Lynda Erskine, Richard S Nowakowski, Carol A Mason

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

In albino mammals, lack of pigment in the retinal pigment epithelium is associated with retinal defects, including poor visual acuity from a photoreceptor deficit in the central retina and poor depth perception from a decrease in ipsilaterally projecting retinal fibers. Possible contributors to these abnormalities are reported delays in neuronogenesis (Ilia and Jeffery, 1996) and retinal maturation (Webster and Rowe, 1991). To further determine possible perturbations in neuronogenesis and/or differentiation, we used cell-specific markers and refined birth dating methods to examine these events during retinal ganglion cell (RGC) genesis in albino and pigmented mice from embryonic day 11 (E11) to E18. Our data indicate that relative to pigmented mice, more ganglion cells are born in the early stages of neuronogenesis in the albino retina, although the initiation of RGC genesis in the albino is unchanged. The cellular organization of the albino retina is perturbed as early as E12. In addition, cell cycle kinetics and output along the nasotemporal axis differ in retinas of albino and pigmented mice, both absolutely, with the temporal aspect of the retina expanded in albino, and relative to the position of the optic nerve head. Finally, blocking melanin synthesis in pigmented eyecups in culture leads to an increase in RGC differentiation, consistent with a role for melanin formation in regulating RGC neuronogenesis. These results point to spatiotemporal defects in neuronal production in the albino retina, which could perturb expression of genes that specify cell fate, number, and/or projection phenotype.
Original languageEnglish
Pages (from-to)4249-4263
Number of pages15
JournalJournal of Neuroscience
Volume22
Issue number11
Publication statusPublished - 1 Jun 2002

Fingerprint

Retina
Retinal Ganglion Cells
Melanins
Cell Differentiation
Ilium
Depth Perception
Retinal Pigment Epithelium
Optic Disk
Ganglia
Visual Acuity
Mammals
Cell Cycle
Cell Count
Parturition
Phenotype
Gene Expression

Keywords

  • Albinism
  • Animals
  • Biotin
  • Bromodeoxyuridine
  • Cell Count
  • Cell Cycle
  • Cell Differentiation
  • Cell Division
  • Dextrans
  • Eye Proteins
  • Flow Cytometry
  • Homeodomain Proteins
  • Immunohistochemistry
  • Melanins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Monophenol Monooxygenase
  • Nerve Tissue Proteins
  • Neurons
  • Retina
  • Retinal Ganglion Cells
  • S Phase
  • Species Specificity
  • albino
  • biotinylated dextran amine
  • bromodeoxyuridine
  • cell cycle
  • flow cytometry
  • Islet 1/2
  • melanin
  • neurogenesis
  • neuronogenesis
  • phenylthiourea
  • retinal ganglion cell
  • retinal pigment epithelium
  • thymidine
  • ventricular zone

Cite this

Rachel, R. A., Dolen, G., Hayes, N. L., Lu, A., Erskine, L., Nowakowski, R. S., & Mason, C. A. (2002). Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina. Journal of Neuroscience, 22(11), 4249-4263.

Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina. / Rachel, Rivka A; Dolen, Gul; Hayes, Nancy L; Lu, Alice; Erskine, Lynda; Nowakowski, Richard S; Mason, Carol A.

In: Journal of Neuroscience, Vol. 22, No. 11, 01.06.2002, p. 4249-4263.

Research output: Contribution to journalArticle

Rachel, RA, Dolen, G, Hayes, NL, Lu, A, Erskine, L, Nowakowski, RS & Mason, CA 2002, 'Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina' Journal of Neuroscience, vol. 22, no. 11, pp. 4249-4263.
Rachel RA, Dolen G, Hayes NL, Lu A, Erskine L, Nowakowski RS et al. Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina. Journal of Neuroscience. 2002 Jun 1;22(11):4249-4263.
Rachel, Rivka A ; Dolen, Gul ; Hayes, Nancy L ; Lu, Alice ; Erskine, Lynda ; Nowakowski, Richard S ; Mason, Carol A. / Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 11. pp. 4249-4263.
@article{9dc41db841ed4527be3ff278422a8d96,
title = "Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina",
abstract = "In albino mammals, lack of pigment in the retinal pigment epithelium is associated with retinal defects, including poor visual acuity from a photoreceptor deficit in the central retina and poor depth perception from a decrease in ipsilaterally projecting retinal fibers. Possible contributors to these abnormalities are reported delays in neuronogenesis (Ilia and Jeffery, 1996) and retinal maturation (Webster and Rowe, 1991). To further determine possible perturbations in neuronogenesis and/or differentiation, we used cell-specific markers and refined birth dating methods to examine these events during retinal ganglion cell (RGC) genesis in albino and pigmented mice from embryonic day 11 (E11) to E18. Our data indicate that relative to pigmented mice, more ganglion cells are born in the early stages of neuronogenesis in the albino retina, although the initiation of RGC genesis in the albino is unchanged. The cellular organization of the albino retina is perturbed as early as E12. In addition, cell cycle kinetics and output along the nasotemporal axis differ in retinas of albino and pigmented mice, both absolutely, with the temporal aspect of the retina expanded in albino, and relative to the position of the optic nerve head. Finally, blocking melanin synthesis in pigmented eyecups in culture leads to an increase in RGC differentiation, consistent with a role for melanin formation in regulating RGC neuronogenesis. These results point to spatiotemporal defects in neuronal production in the albino retina, which could perturb expression of genes that specify cell fate, number, and/or projection phenotype.",
keywords = "Albinism, Animals, Biotin, Bromodeoxyuridine, Cell Count, Cell Cycle, Cell Differentiation, Cell Division, Dextrans, Eye Proteins, Flow Cytometry, Homeodomain Proteins, Immunohistochemistry, Melanins, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Monophenol Monooxygenase, Nerve Tissue Proteins, Neurons, Retina, Retinal Ganglion Cells, S Phase, Species Specificity, albino, biotinylated dextran amine, bromodeoxyuridine, cell cycle, flow cytometry, Islet 1/2, melanin, neurogenesis, neuronogenesis, phenylthiourea, retinal ganglion cell, retinal pigment epithelium, thymidine, ventricular zone",
author = "Rachel, {Rivka A} and Gul Dolen and Hayes, {Nancy L} and Alice Lu and Lynda Erskine and Nowakowski, {Richard S} and Mason, {Carol A}",
year = "2002",
month = "6",
day = "1",
language = "English",
volume = "22",
pages = "4249--4263",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "11",

}

TY - JOUR

T1 - Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina

AU - Rachel, Rivka A

AU - Dolen, Gul

AU - Hayes, Nancy L

AU - Lu, Alice

AU - Erskine, Lynda

AU - Nowakowski, Richard S

AU - Mason, Carol A

PY - 2002/6/1

Y1 - 2002/6/1

N2 - In albino mammals, lack of pigment in the retinal pigment epithelium is associated with retinal defects, including poor visual acuity from a photoreceptor deficit in the central retina and poor depth perception from a decrease in ipsilaterally projecting retinal fibers. Possible contributors to these abnormalities are reported delays in neuronogenesis (Ilia and Jeffery, 1996) and retinal maturation (Webster and Rowe, 1991). To further determine possible perturbations in neuronogenesis and/or differentiation, we used cell-specific markers and refined birth dating methods to examine these events during retinal ganglion cell (RGC) genesis in albino and pigmented mice from embryonic day 11 (E11) to E18. Our data indicate that relative to pigmented mice, more ganglion cells are born in the early stages of neuronogenesis in the albino retina, although the initiation of RGC genesis in the albino is unchanged. The cellular organization of the albino retina is perturbed as early as E12. In addition, cell cycle kinetics and output along the nasotemporal axis differ in retinas of albino and pigmented mice, both absolutely, with the temporal aspect of the retina expanded in albino, and relative to the position of the optic nerve head. Finally, blocking melanin synthesis in pigmented eyecups in culture leads to an increase in RGC differentiation, consistent with a role for melanin formation in regulating RGC neuronogenesis. These results point to spatiotemporal defects in neuronal production in the albino retina, which could perturb expression of genes that specify cell fate, number, and/or projection phenotype.

AB - In albino mammals, lack of pigment in the retinal pigment epithelium is associated with retinal defects, including poor visual acuity from a photoreceptor deficit in the central retina and poor depth perception from a decrease in ipsilaterally projecting retinal fibers. Possible contributors to these abnormalities are reported delays in neuronogenesis (Ilia and Jeffery, 1996) and retinal maturation (Webster and Rowe, 1991). To further determine possible perturbations in neuronogenesis and/or differentiation, we used cell-specific markers and refined birth dating methods to examine these events during retinal ganglion cell (RGC) genesis in albino and pigmented mice from embryonic day 11 (E11) to E18. Our data indicate that relative to pigmented mice, more ganglion cells are born in the early stages of neuronogenesis in the albino retina, although the initiation of RGC genesis in the albino is unchanged. The cellular organization of the albino retina is perturbed as early as E12. In addition, cell cycle kinetics and output along the nasotemporal axis differ in retinas of albino and pigmented mice, both absolutely, with the temporal aspect of the retina expanded in albino, and relative to the position of the optic nerve head. Finally, blocking melanin synthesis in pigmented eyecups in culture leads to an increase in RGC differentiation, consistent with a role for melanin formation in regulating RGC neuronogenesis. These results point to spatiotemporal defects in neuronal production in the albino retina, which could perturb expression of genes that specify cell fate, number, and/or projection phenotype.

KW - Albinism

KW - Animals

KW - Biotin

KW - Bromodeoxyuridine

KW - Cell Count

KW - Cell Cycle

KW - Cell Differentiation

KW - Cell Division

KW - Dextrans

KW - Eye Proteins

KW - Flow Cytometry

KW - Homeodomain Proteins

KW - Immunohistochemistry

KW - Melanins

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Mutant Strains

KW - Monophenol Monooxygenase

KW - Nerve Tissue Proteins

KW - Neurons

KW - Retina

KW - Retinal Ganglion Cells

KW - S Phase

KW - Species Specificity

KW - albino

KW - biotinylated dextran amine

KW - bromodeoxyuridine

KW - cell cycle

KW - flow cytometry

KW - Islet 1/2

KW - melanin

KW - neurogenesis

KW - neuronogenesis

KW - phenylthiourea

KW - retinal ganglion cell

KW - retinal pigment epithelium

KW - thymidine

KW - ventricular zone

M3 - Article

VL - 22

SP - 4249

EP - 4263

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 11

ER -