Species-specific primers discriminate schistosome intermediate hosts: unambiguous PCR diagnosis of Bulinus forskalii group taxa (Gastropoda: Planorbidae)

Catherine Sue Jones, Leslie Robert Noble, Anne Elizabeth Lockyer, D S Brown, D Rollinson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The morphological definition of taxa has proved difficult within the Bulinus forskalii group, which includes intermediate hosts of medically important Schistosoma species in West Africa. Although B. forskalii and B. senegalensis transmit different schistosome species they are conchologically similar and their distributions overlap. Randomly amplified polymorphic DNA (RAPD) allows differentiation of sibling species in the genus Bulinus, but RAPDs are difficult to standardize, impairing their value as a taxonomic tool. Hence, RAPD products diagnostic for either B. senegalensis or B. forskalii from West Africa were cloned, sequenced and a panel of species-specific primers designed. Sequencing of RAPD products identified a homology in two apparently independent RAPD loci, a problem where RAPDs are indiscriminately scored for phylogenetic analyses. Specificity of primers was confirmed by widespread sampling throughout each species' range. This approach produced a simple, robust, unambiguous PCR-based species identification strategy for this difficult group.

Original languageEnglish
Pages (from-to)843-849
Number of pages7
JournalMolecular Ecology
Volume6
Issue number9
Publication statusPublished - Sep 1997

Keywords

  • RAPDs
  • species-specific primers
  • PCR
  • tropical freshwater snails
  • B-forskalii
  • POLYMORPHIC DNA RAPD
  • INTERNAL TRANSCRIBED SPACER
  • GENUS BULINUS
  • ARBITRARY PRIMERS
  • MARKERS
  • IDENTIFICATION
  • CAMEROON
  • PROBES
  • GENES

Cite this

Species-specific primers discriminate schistosome intermediate hosts: unambiguous PCR diagnosis of Bulinus forskalii group taxa (Gastropoda: Planorbidae). / Jones, Catherine Sue; Noble, Leslie Robert; Lockyer, Anne Elizabeth; Brown, D S ; Rollinson, D .

In: Molecular Ecology, Vol. 6, No. 9, 09.1997, p. 843-849.

Research output: Contribution to journalArticle

@article{5815c57e63d04200a53ed86d9e8f4f9f,
title = "Species-specific primers discriminate schistosome intermediate hosts: unambiguous PCR diagnosis of Bulinus forskalii group taxa (Gastropoda: Planorbidae)",
abstract = "The morphological definition of taxa has proved difficult within the Bulinus forskalii group, which includes intermediate hosts of medically important Schistosoma species in West Africa. Although B. forskalii and B. senegalensis transmit different schistosome species they are conchologically similar and their distributions overlap. Randomly amplified polymorphic DNA (RAPD) allows differentiation of sibling species in the genus Bulinus, but RAPDs are difficult to standardize, impairing their value as a taxonomic tool. Hence, RAPD products diagnostic for either B. senegalensis or B. forskalii from West Africa were cloned, sequenced and a panel of species-specific primers designed. Sequencing of RAPD products identified a homology in two apparently independent RAPD loci, a problem where RAPDs are indiscriminately scored for phylogenetic analyses. Specificity of primers was confirmed by widespread sampling throughout each species' range. This approach produced a simple, robust, unambiguous PCR-based species identification strategy for this difficult group.",
keywords = "RAPDs, species-specific primers, PCR, tropical freshwater snails, B-forskalii, POLYMORPHIC DNA RAPD, INTERNAL TRANSCRIBED SPACER, GENUS BULINUS, ARBITRARY PRIMERS, MARKERS, IDENTIFICATION, CAMEROON, PROBES, GENES",
author = "Jones, {Catherine Sue} and Noble, {Leslie Robert} and Lockyer, {Anne Elizabeth} and Brown, {D S} and D Rollinson",
year = "1997",
month = "9",
language = "English",
volume = "6",
pages = "843--849",
journal = "Molecular Ecology",
issn = "0962-1083",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Species-specific primers discriminate schistosome intermediate hosts: unambiguous PCR diagnosis of Bulinus forskalii group taxa (Gastropoda: Planorbidae)

AU - Jones, Catherine Sue

AU - Noble, Leslie Robert

AU - Lockyer, Anne Elizabeth

AU - Brown, D S

AU - Rollinson, D

PY - 1997/9

Y1 - 1997/9

N2 - The morphological definition of taxa has proved difficult within the Bulinus forskalii group, which includes intermediate hosts of medically important Schistosoma species in West Africa. Although B. forskalii and B. senegalensis transmit different schistosome species they are conchologically similar and their distributions overlap. Randomly amplified polymorphic DNA (RAPD) allows differentiation of sibling species in the genus Bulinus, but RAPDs are difficult to standardize, impairing their value as a taxonomic tool. Hence, RAPD products diagnostic for either B. senegalensis or B. forskalii from West Africa were cloned, sequenced and a panel of species-specific primers designed. Sequencing of RAPD products identified a homology in two apparently independent RAPD loci, a problem where RAPDs are indiscriminately scored for phylogenetic analyses. Specificity of primers was confirmed by widespread sampling throughout each species' range. This approach produced a simple, robust, unambiguous PCR-based species identification strategy for this difficult group.

AB - The morphological definition of taxa has proved difficult within the Bulinus forskalii group, which includes intermediate hosts of medically important Schistosoma species in West Africa. Although B. forskalii and B. senegalensis transmit different schistosome species they are conchologically similar and their distributions overlap. Randomly amplified polymorphic DNA (RAPD) allows differentiation of sibling species in the genus Bulinus, but RAPDs are difficult to standardize, impairing their value as a taxonomic tool. Hence, RAPD products diagnostic for either B. senegalensis or B. forskalii from West Africa were cloned, sequenced and a panel of species-specific primers designed. Sequencing of RAPD products identified a homology in two apparently independent RAPD loci, a problem where RAPDs are indiscriminately scored for phylogenetic analyses. Specificity of primers was confirmed by widespread sampling throughout each species' range. This approach produced a simple, robust, unambiguous PCR-based species identification strategy for this difficult group.

KW - RAPDs

KW - species-specific primers

KW - PCR

KW - tropical freshwater snails

KW - B-forskalii

KW - POLYMORPHIC DNA RAPD

KW - INTERNAL TRANSCRIBED SPACER

KW - GENUS BULINUS

KW - ARBITRARY PRIMERS

KW - MARKERS

KW - IDENTIFICATION

KW - CAMEROON

KW - PROBES

KW - GENES

M3 - Article

VL - 6

SP - 843

EP - 849

JO - Molecular Ecology

JF - Molecular Ecology

SN - 0962-1083

IS - 9

ER -