Spliced leader trans-splicing in the nematode Trichinella spiralis uses highly polymorphic, noncanonical spliced leaders

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The trans-splicing of short spliced leader (SL) RNAs onto the 5' ends of mRNAs occurs in a diverse range of taxa. In nematodes, all species so far characterized utilize a characteristic, conserved spliced leader, SL1, as well as variants that are employed in the resolution of operons. Here we report the identification of spliced leader trans-splicing in the basal nematode Trichinella spiralis, and show that this nematode does not possess a canonical SL1, but rather has at least 15 distinct spliced leaders, encoded by at least 19 SL RNA genes. The individual spliced leaders vary in both size and primary sequence, showing a much higher degree of diversity compared to other known trans-spliced leaders. In a survey of T. spiralis mRNAs, individual mRNAs were found to be trans-spliced to a number of different spliced leader sequences. These data provide the first indication that the last common ancestor of the phylum Nematoda utilized spliced leader trans-splicing and that the canonical spliced leader, SL1, found in Caenorhabditis elegans, evolved after the divergence of the major nematode clades. This discovery sheds important light on the nature and evolution of mRNA processing in the Nematoda.
Original languageEnglish
Pages (from-to)760-770
Number of pages11
JournalRNA
Volume14
Issue number4
Early online date6 Feb 2008
DOIs
Publication statusPublished - 2008

Keywords

  • 5' Untranslated Regions
  • Animals
  • Base Sequence
  • DNA, Complementary
  • DNA, Helminth
  • Expressed Sequence Tags
  • Genes, Helminth
  • Molecular Sequence Data
  • Phylogeny
  • Polymorphism, Genetic
  • RNA, Helminth
  • RNA, Spliced Leader
  • Trans-Splicing
  • Trichinella spiralis

Cite this

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title = "Spliced leader trans-splicing in the nematode Trichinella spiralis uses highly polymorphic, noncanonical spliced leaders",
abstract = "The trans-splicing of short spliced leader (SL) RNAs onto the 5' ends of mRNAs occurs in a diverse range of taxa. In nematodes, all species so far characterized utilize a characteristic, conserved spliced leader, SL1, as well as variants that are employed in the resolution of operons. Here we report the identification of spliced leader trans-splicing in the basal nematode Trichinella spiralis, and show that this nematode does not possess a canonical SL1, but rather has at least 15 distinct spliced leaders, encoded by at least 19 SL RNA genes. The individual spliced leaders vary in both size and primary sequence, showing a much higher degree of diversity compared to other known trans-spliced leaders. In a survey of T. spiralis mRNAs, individual mRNAs were found to be trans-spliced to a number of different spliced leader sequences. These data provide the first indication that the last common ancestor of the phylum Nematoda utilized spliced leader trans-splicing and that the canonical spliced leader, SL1, found in Caenorhabditis elegans, evolved after the divergence of the major nematode clades. This discovery sheds important light on the nature and evolution of mRNA processing in the Nematoda.",
keywords = "5' Untranslated Regions, Animals, Base Sequence, DNA, Complementary, DNA, Helminth, Expressed Sequence Tags, Genes, Helminth, Molecular Sequence Data, Phylogeny, Polymorphism, Genetic, RNA, Helminth, RNA, Spliced Leader, Trans-Splicing, Trichinella spiralis",
author = "Jonathan Pettitt and Muller, {Berndt Marino} and Ian Stansfield and Bernadette Connolly",
year = "2008",
doi = "10.1261/rna.948008",
language = "English",
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pages = "760--770",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
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T1 - Spliced leader trans-splicing in the nematode Trichinella spiralis uses highly polymorphic, noncanonical spliced leaders

AU - Pettitt, Jonathan

AU - Muller, Berndt Marino

AU - Stansfield, Ian

AU - Connolly, Bernadette

PY - 2008

Y1 - 2008

N2 - The trans-splicing of short spliced leader (SL) RNAs onto the 5' ends of mRNAs occurs in a diverse range of taxa. In nematodes, all species so far characterized utilize a characteristic, conserved spliced leader, SL1, as well as variants that are employed in the resolution of operons. Here we report the identification of spliced leader trans-splicing in the basal nematode Trichinella spiralis, and show that this nematode does not possess a canonical SL1, but rather has at least 15 distinct spliced leaders, encoded by at least 19 SL RNA genes. The individual spliced leaders vary in both size and primary sequence, showing a much higher degree of diversity compared to other known trans-spliced leaders. In a survey of T. spiralis mRNAs, individual mRNAs were found to be trans-spliced to a number of different spliced leader sequences. These data provide the first indication that the last common ancestor of the phylum Nematoda utilized spliced leader trans-splicing and that the canonical spliced leader, SL1, found in Caenorhabditis elegans, evolved after the divergence of the major nematode clades. This discovery sheds important light on the nature and evolution of mRNA processing in the Nematoda.

AB - The trans-splicing of short spliced leader (SL) RNAs onto the 5' ends of mRNAs occurs in a diverse range of taxa. In nematodes, all species so far characterized utilize a characteristic, conserved spliced leader, SL1, as well as variants that are employed in the resolution of operons. Here we report the identification of spliced leader trans-splicing in the basal nematode Trichinella spiralis, and show that this nematode does not possess a canonical SL1, but rather has at least 15 distinct spliced leaders, encoded by at least 19 SL RNA genes. The individual spliced leaders vary in both size and primary sequence, showing a much higher degree of diversity compared to other known trans-spliced leaders. In a survey of T. spiralis mRNAs, individual mRNAs were found to be trans-spliced to a number of different spliced leader sequences. These data provide the first indication that the last common ancestor of the phylum Nematoda utilized spliced leader trans-splicing and that the canonical spliced leader, SL1, found in Caenorhabditis elegans, evolved after the divergence of the major nematode clades. This discovery sheds important light on the nature and evolution of mRNA processing in the Nematoda.

KW - 5' Untranslated Regions

KW - Animals

KW - Base Sequence

KW - DNA, Complementary

KW - DNA, Helminth

KW - Expressed Sequence Tags

KW - Genes, Helminth

KW - Molecular Sequence Data

KW - Phylogeny

KW - Polymorphism, Genetic

KW - RNA, Helminth

KW - RNA, Spliced Leader

KW - Trans-Splicing

KW - Trichinella spiralis

U2 - 10.1261/rna.948008

DO - 10.1261/rna.948008

M3 - Article

VL - 14

SP - 760

EP - 770

JO - RNA

JF - RNA

SN - 1355-8382

IS - 4

ER -