Stabilization of antibody fragments in adverse environments

Helen Dooley, Steven D. Grant, William J. Harris, Andrew J. Porter

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Antibody fragments have the potential to be used as sensitive and specific binding agents in a broad range of industrial applications. Genetic manipulation has been used to design a series of antibody fragment configurations with a flexible linker and/or a disulphide bond between the heavy chain and light chain of an antibody fragment against the herbicide atrazine. The thermostability and stability to a range of denaturants, polar and non-polar solvents, surfactants and proteases have been compared, it has been found that a novel antibody fragment construct (STAB: stabilized antibody) containing both a flexible linker and a disulphide bond can be effectively produced and shows greatly improved stability in these diverse environments. These STABs should be useful in environmental diagnostics and remediation, and may provide a generic approach for stabilizing antibody fragments in formulations containing detergents and penetrants for topical application in the pharmaceutical and cosmetic industries.
Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalBiotechnology and Applied Biochemistry
Volume28
Issue number1
Publication statusPublished - 20 Aug 1998

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