Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis

Sigrid E M Heinsbroek, Philip R Taylor, Fernando O Martinez, Luisa Martinez-Pomares, Gordon D Brown, Siamon Gordon

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Abstract

Candida albicans is a medically important pathogen, and recognition by innate immune cells is critical for its clearance. Although a number of pattern recognition receptors have been shown to be involved in recognition and phagocytosis of this fungus, the relative role of these receptors has not been formally examined. In this paper, we have investigated the contribution of the mannose receptor, Dectin-1, and complement receptor 3; and we have demonstrated that Dectin-1 is the main non-opsonic receptor involved in fungal uptake. However, both Dectin-1 and complement receptor 3 were found to accumulate at the site of uptake, while mannose receptor accumulated on C. albicans phagosomes at later stages. These results suggest a potential role for MR in phagosome sampling; and, accordingly, MR deficiency led to a reduction in TNF-alpha and MCP-1 production in response to C. albicans uptake. Our data suggest that pattern recognition receptors sample the fungal phagosome in a sequential fashion.
Original languageEnglish
Article numbere1000218
Number of pages11
JournalPLoS Pathogens
Volume4
Issue number11
DOIs
Publication statusPublished - 28 Nov 2008

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Pattern Recognition Receptors
Phagosomes
Candida albicans
Phagocytosis
Macrophage-1 Antigen
Fungi
Tumor Necrosis Factor-alpha
dectin 1
mannose receptor

Keywords

  • animals
  • Candida albicans
  • humans
  • lectins, C-type
  • macrophage-1 antigen
  • mannose-binding lectins
  • membrane proteins
  • mice
  • nerve tissue proteins
  • phagocytosis
  • phagosomes
  • receptors, cell surface
  • receptors, pattern recognition
  • time factors

Cite this

Heinsbroek, S. E. M., Taylor, P. R., Martinez, F. O., Martinez-Pomares, L., Brown, G. D., & Gordon, S. (2008). Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis. PLoS Pathogens, 4(11), [e1000218]. https://doi.org/10.1371/journal.ppat.1000218

Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis. / Heinsbroek, Sigrid E M; Taylor, Philip R; Martinez, Fernando O; Martinez-Pomares, Luisa; Brown, Gordon D; Gordon, Siamon .

In: PLoS Pathogens, Vol. 4, No. 11, e1000218, 28.11.2008.

Research output: Contribution to journalArticle

Heinsbroek, SEM, Taylor, PR, Martinez, FO, Martinez-Pomares, L, Brown, GD & Gordon, S 2008, 'Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis', PLoS Pathogens, vol. 4, no. 11, e1000218. https://doi.org/10.1371/journal.ppat.1000218
Heinsbroek SEM, Taylor PR, Martinez FO, Martinez-Pomares L, Brown GD, Gordon S. Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis. PLoS Pathogens. 2008 Nov 28;4(11). e1000218. https://doi.org/10.1371/journal.ppat.1000218
Heinsbroek, Sigrid E M ; Taylor, Philip R ; Martinez, Fernando O ; Martinez-Pomares, Luisa ; Brown, Gordon D ; Gordon, Siamon . / Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis. In: PLoS Pathogens. 2008 ; Vol. 4, No. 11.
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