STAT3 protein interacts with Class O Forkhead transcription factors in the cytoplasm and regulates nuclear/cytoplasmic localization of FoxO1 and FoxO3a proteins in CD4(+) T cells

Hyun-Mee Oh, Cheng-Rong Yu, Ivy Dambuza, Bernadette Marrero, Charles E Egwuagu

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

An important feature of the adaptive immune response is its remarkable capacity to regulate the duration of inflammatory responses, and effector T cells have been shown to limit excessive immune responses by producing anti-inflammatory cytokines such as IL-10 and IL-27. However, how anti-inflammatory cytokines mediate their suppressive activities is not well understood. In this study, we show that STAT3 contributes to mechanisms that control the duration of T cell proliferation by regulating the subcellular location of FoxO1 and FoxO3a, two Class O Forkhead transcription factors that mediate lymphocyte quiescence and inhibit T cell activation. We show that active FoxO1 and FoxO3a reside exclusively in the nucleus of naïve T cells whereas inactive pFoxO1 and pFoxO3a were most abundant in activated T cells and sequestered in their cytoplasm in association with unphosphorylated STAT3 (U-STAT3) and 14-3-3. We further show that FoxO1/FoxO3a rapidly relocalized into the nucleus in response to pSTAT3 activation by IL-6 or IL-10, and the accumulation of FoxO1/FoxO3a in their nuclei coincided with increased expression of p27(Kip1) and p21(WAF1). STAT3 inhibitors completely abrogated cytokine-induced translocation of FoxO1/FoxO3a into the nucleus. In naïve or resting STAT3-deficient T cells, expression of pFoxO1/pFoxO3a was predominantly in the cytoplasm and correlated with defects in p27(Kip1) and p21(WAF1) expression, suggesting requirement of STAT3 for importation or retention of FoxO in the nucleus and attenuation of lymphocyte proliferation. Taken together, these results suggest that U-STAT3 collaborates with 14-3-3 to sequester pFoxO1/pFoxO3a in cytoplasm and thus prolong T cell activation, whereas pSTAT3 activation by anti-inflammatory cytokines would curtail the duration of TCR activation and re-establish lymphocyte quiescence by inducing nuclear localization of FoxO1/FoxO3a and FoxO-mediated expression of growth-inhibitory proteins.

Original languageEnglish
Pages (from-to)30436-30443
Number of pages8
JournalThe Journal of Biological Chemistry
Volume287
Issue number36
Early online date2 Jul 2012
DOIs
Publication statusPublished - 31 Aug 2012

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Forkhead Transcription Factors
STAT3 Transcription Factor
T-cells
Cytoplasm
T-Lymphocytes
Chemical activation
Lymphocytes
Proteins
Cytokines
Anti-Inflammatory Agents
Interleukin-10
Interleukin-27
Cell proliferation
Adaptive Immunity
Interleukin-6
Cell Proliferation
Association reactions
Defects

Keywords

  • 14-3-3 Proteins/genetics
  • Active Transport, Cell Nucleus/immunology
  • Animals
  • CD4-Positive T-Lymphocytes/cytology
  • Cell Nucleus/genetics
  • Cyclin-Dependent Kinase Inhibitor p21/genetics
  • Cyclin-Dependent Kinase Inhibitor p27/genetics
  • Cytokines/genetics
  • Cytoplasm/genetics
  • Forkhead Box Protein O1
  • Forkhead Box Protein O3
  • Forkhead Transcription Factors/genetics
  • Gene Expression Regulation/immunology
  • Humans
  • Jurkat Cells
  • Lymphocyte Activation/physiology
  • Mice
  • Mice, Knockout
  • Receptors, Antigen, T-Cell/genetics
  • STAT3 Transcription Factor/genetics

Cite this

STAT3 protein interacts with Class O Forkhead transcription factors in the cytoplasm and regulates nuclear/cytoplasmic localization of FoxO1 and FoxO3a proteins in CD4(+) T cells. / Oh, Hyun-Mee; Yu, Cheng-Rong; Dambuza, Ivy; Marrero, Bernadette; Egwuagu, Charles E.

In: The Journal of Biological Chemistry, Vol. 287, No. 36, 31.08.2012, p. 30436-30443.

Research output: Contribution to journalArticle

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title = "STAT3 protein interacts with Class O Forkhead transcription factors in the cytoplasm and regulates nuclear/cytoplasmic localization of FoxO1 and FoxO3a proteins in CD4(+) T cells",
abstract = "An important feature of the adaptive immune response is its remarkable capacity to regulate the duration of inflammatory responses, and effector T cells have been shown to limit excessive immune responses by producing anti-inflammatory cytokines such as IL-10 and IL-27. However, how anti-inflammatory cytokines mediate their suppressive activities is not well understood. In this study, we show that STAT3 contributes to mechanisms that control the duration of T cell proliferation by regulating the subcellular location of FoxO1 and FoxO3a, two Class O Forkhead transcription factors that mediate lymphocyte quiescence and inhibit T cell activation. We show that active FoxO1 and FoxO3a reside exclusively in the nucleus of na{\"i}ve T cells whereas inactive pFoxO1 and pFoxO3a were most abundant in activated T cells and sequestered in their cytoplasm in association with unphosphorylated STAT3 (U-STAT3) and 14-3-3. We further show that FoxO1/FoxO3a rapidly relocalized into the nucleus in response to pSTAT3 activation by IL-6 or IL-10, and the accumulation of FoxO1/FoxO3a in their nuclei coincided with increased expression of p27(Kip1) and p21(WAF1). STAT3 inhibitors completely abrogated cytokine-induced translocation of FoxO1/FoxO3a into the nucleus. In na{\"i}ve or resting STAT3-deficient T cells, expression of pFoxO1/pFoxO3a was predominantly in the cytoplasm and correlated with defects in p27(Kip1) and p21(WAF1) expression, suggesting requirement of STAT3 for importation or retention of FoxO in the nucleus and attenuation of lymphocyte proliferation. Taken together, these results suggest that U-STAT3 collaborates with 14-3-3 to sequester pFoxO1/pFoxO3a in cytoplasm and thus prolong T cell activation, whereas pSTAT3 activation by anti-inflammatory cytokines would curtail the duration of TCR activation and re-establish lymphocyte quiescence by inducing nuclear localization of FoxO1/FoxO3a and FoxO-mediated expression of growth-inhibitory proteins.",
keywords = "14-3-3 Proteins/genetics, Active Transport, Cell Nucleus/immunology, Animals, CD4-Positive T-Lymphocytes/cytology, Cell Nucleus/genetics, Cyclin-Dependent Kinase Inhibitor p21/genetics, Cyclin-Dependent Kinase Inhibitor p27/genetics, Cytokines/genetics, Cytoplasm/genetics, Forkhead Box Protein O1, Forkhead Box Protein O3, Forkhead Transcription Factors/genetics, Gene Expression Regulation/immunology, Humans, Jurkat Cells, Lymphocyte Activation/physiology, Mice, Mice, Knockout, Receptors, Antigen, T-Cell/genetics, STAT3 Transcription Factor/genetics",
author = "Hyun-Mee Oh and Cheng-Rong Yu and Ivy Dambuza and Bernadette Marrero and Egwuagu, {Charles E}",
note = "Acknowledgements—We thank Dr. Terry Unterman (Northwestern University Medical School, Chicago) for providing FoxO1-GFP plasmids, Dr. Robert Fariss (NEI Institute Imaging Facility, National Institutes of Health) for assistance with confocal microscopy, Rashid M. Mahdi (Molecular Immunology Section, NEI, National Institutes of Health) for technical assistance, and Dr. Li Zhang (Molecular Immunology Section, NEI, National Institutes of Health) for critical reading of the manuscript.",
year = "2012",
month = "8",
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doi = "10.1074/jbc.M112.359661",
language = "English",
volume = "287",
pages = "30436--30443",
journal = "The Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC",
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TY - JOUR

T1 - STAT3 protein interacts with Class O Forkhead transcription factors in the cytoplasm and regulates nuclear/cytoplasmic localization of FoxO1 and FoxO3a proteins in CD4(+) T cells

AU - Oh, Hyun-Mee

AU - Yu, Cheng-Rong

AU - Dambuza, Ivy

AU - Marrero, Bernadette

AU - Egwuagu, Charles E

N1 - Acknowledgements—We thank Dr. Terry Unterman (Northwestern University Medical School, Chicago) for providing FoxO1-GFP plasmids, Dr. Robert Fariss (NEI Institute Imaging Facility, National Institutes of Health) for assistance with confocal microscopy, Rashid M. Mahdi (Molecular Immunology Section, NEI, National Institutes of Health) for technical assistance, and Dr. Li Zhang (Molecular Immunology Section, NEI, National Institutes of Health) for critical reading of the manuscript.

PY - 2012/8/31

Y1 - 2012/8/31

N2 - An important feature of the adaptive immune response is its remarkable capacity to regulate the duration of inflammatory responses, and effector T cells have been shown to limit excessive immune responses by producing anti-inflammatory cytokines such as IL-10 and IL-27. However, how anti-inflammatory cytokines mediate their suppressive activities is not well understood. In this study, we show that STAT3 contributes to mechanisms that control the duration of T cell proliferation by regulating the subcellular location of FoxO1 and FoxO3a, two Class O Forkhead transcription factors that mediate lymphocyte quiescence and inhibit T cell activation. We show that active FoxO1 and FoxO3a reside exclusively in the nucleus of naïve T cells whereas inactive pFoxO1 and pFoxO3a were most abundant in activated T cells and sequestered in their cytoplasm in association with unphosphorylated STAT3 (U-STAT3) and 14-3-3. We further show that FoxO1/FoxO3a rapidly relocalized into the nucleus in response to pSTAT3 activation by IL-6 or IL-10, and the accumulation of FoxO1/FoxO3a in their nuclei coincided with increased expression of p27(Kip1) and p21(WAF1). STAT3 inhibitors completely abrogated cytokine-induced translocation of FoxO1/FoxO3a into the nucleus. In naïve or resting STAT3-deficient T cells, expression of pFoxO1/pFoxO3a was predominantly in the cytoplasm and correlated with defects in p27(Kip1) and p21(WAF1) expression, suggesting requirement of STAT3 for importation or retention of FoxO in the nucleus and attenuation of lymphocyte proliferation. Taken together, these results suggest that U-STAT3 collaborates with 14-3-3 to sequester pFoxO1/pFoxO3a in cytoplasm and thus prolong T cell activation, whereas pSTAT3 activation by anti-inflammatory cytokines would curtail the duration of TCR activation and re-establish lymphocyte quiescence by inducing nuclear localization of FoxO1/FoxO3a and FoxO-mediated expression of growth-inhibitory proteins.

AB - An important feature of the adaptive immune response is its remarkable capacity to regulate the duration of inflammatory responses, and effector T cells have been shown to limit excessive immune responses by producing anti-inflammatory cytokines such as IL-10 and IL-27. However, how anti-inflammatory cytokines mediate their suppressive activities is not well understood. In this study, we show that STAT3 contributes to mechanisms that control the duration of T cell proliferation by regulating the subcellular location of FoxO1 and FoxO3a, two Class O Forkhead transcription factors that mediate lymphocyte quiescence and inhibit T cell activation. We show that active FoxO1 and FoxO3a reside exclusively in the nucleus of naïve T cells whereas inactive pFoxO1 and pFoxO3a were most abundant in activated T cells and sequestered in their cytoplasm in association with unphosphorylated STAT3 (U-STAT3) and 14-3-3. We further show that FoxO1/FoxO3a rapidly relocalized into the nucleus in response to pSTAT3 activation by IL-6 or IL-10, and the accumulation of FoxO1/FoxO3a in their nuclei coincided with increased expression of p27(Kip1) and p21(WAF1). STAT3 inhibitors completely abrogated cytokine-induced translocation of FoxO1/FoxO3a into the nucleus. In naïve or resting STAT3-deficient T cells, expression of pFoxO1/pFoxO3a was predominantly in the cytoplasm and correlated with defects in p27(Kip1) and p21(WAF1) expression, suggesting requirement of STAT3 for importation or retention of FoxO in the nucleus and attenuation of lymphocyte proliferation. Taken together, these results suggest that U-STAT3 collaborates with 14-3-3 to sequester pFoxO1/pFoxO3a in cytoplasm and thus prolong T cell activation, whereas pSTAT3 activation by anti-inflammatory cytokines would curtail the duration of TCR activation and re-establish lymphocyte quiescence by inducing nuclear localization of FoxO1/FoxO3a and FoxO-mediated expression of growth-inhibitory proteins.

KW - 14-3-3 Proteins/genetics

KW - Active Transport, Cell Nucleus/immunology

KW - Animals

KW - CD4-Positive T-Lymphocytes/cytology

KW - Cell Nucleus/genetics

KW - Cyclin-Dependent Kinase Inhibitor p21/genetics

KW - Cyclin-Dependent Kinase Inhibitor p27/genetics

KW - Cytokines/genetics

KW - Cytoplasm/genetics

KW - Forkhead Box Protein O1

KW - Forkhead Box Protein O3

KW - Forkhead Transcription Factors/genetics

KW - Gene Expression Regulation/immunology

KW - Humans

KW - Jurkat Cells

KW - Lymphocyte Activation/physiology

KW - Mice

KW - Mice, Knockout

KW - Receptors, Antigen, T-Cell/genetics

KW - STAT3 Transcription Factor/genetics

U2 - 10.1074/jbc.M112.359661

DO - 10.1074/jbc.M112.359661

M3 - Article

VL - 287

SP - 30436

EP - 30443

JO - The Journal of Biological Chemistry

JF - The Journal of Biological Chemistry

SN - 0021-9258

IS - 36

ER -