Abstract
Methods: One hundred and thirty-two rats and mice were used. Energy balance was measured by a comprehensive laboratory animal monitoring system. Gene expression was analysed by in situ hybridisation in M3KO mice.
Results: DIO rats reached the plateau of body weight 28 weeks after starting high-fat diet (25% heavier than controls), whereas DIO mice reached the plateau after 6 weeks (23% heavier than controls). At the plateau, DIO rats had higher calorie intake during the light phase but not during the dark phase, while mice had the same calorie intake per day as controls. DIO rats and mice had lower energy expenditure (EE) and respiratory exchange ratio (RER) than controls. GB-rats reached the plateau (15% weight loss) 2 weeks after surgery and had the same calorie intake as sham-operated controls. EE, but not RER, was higher in GB rats than controls during the dark phase. The lean M3KO mice (25% lighter than wild-type (WT) mice at the plateau between 6 and 15 months of age) had the same calorie intake but higher EE, RER and hypothalamic mRNA expression of NPY, AgRP and leptin receptor than WT mice.
Conclusion: When body weight gain or loss reached a plateau, the steady-state energy balance was mainly maintained by EE and/or RER rather than calorie intake.
| Original language | English |
|---|---|
| Pages (from-to) | 442-449 |
| Number of pages | 8 |
| Journal | Scandinavian Journal of Gastroenterology |
| Volume | 52 |
| Issue number | 4 |
| Early online date | 20 Dec 2016 |
| DOIs | |
| Publication status | Published - 2017 |
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Keywords
- eating behaviour
- high-fat diet
- gastric bypass
- Muscarinic acetylcholine M3 receptor knockout
- mice
- rats
Cite this
Steady-state energy balance in animal models of obesity and weight loss. / Olsen, Magnus Kringstad ; Johannessen, Helene; Cassie, Nikki; Barrett, Perry; Takeuchi, Koji ; Kulseng, Bård; Chen, Duan; Zhao, Chun-Mei .
In: Scandinavian Journal of Gastroenterology, Vol. 52, No. 4, 2017, p. 442-449.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Steady-state energy balance in animal models of obesity and weight loss
AU - Olsen, Magnus Kringstad
AU - Johannessen, Helene
AU - Cassie, Nikki
AU - Barrett, Perry
AU - Takeuchi, Koji
AU - Kulseng, Bård
AU - Chen, Duan
AU - Zhao, Chun-Mei
N1 - Supported by European Union Seventh Framework Programme (FP7/2007-2013, n°266408 (Full4Health)), the Joint Programme of the Medical Faculty of Norwegian University of Science and Technology (NTNU) and St. Olav’s University Hospital, the Liaison Committee between the Central Norway Regional Health Authority and NTNU.
PY - 2017
Y1 - 2017
N2 - Objective: We wanted to exam the steady-state energy balance by using high-fat diet-induced obese (DIO) rats and mice as models for positive energy balance, and gastric bypassed (GB) rats and gene knockout of muscarinic acetylcholine M3 receptor (M3KO) mice as models for negative energy balance.Methods: One hundred and thirty-two rats and mice were used. Energy balance was measured by a comprehensive laboratory animal monitoring system. Gene expression was analysed by in situ hybridisation in M3KO mice.Results: DIO rats reached the plateau of body weight 28 weeks after starting high-fat diet (25% heavier than controls), whereas DIO mice reached the plateau after 6 weeks (23% heavier than controls). At the plateau, DIO rats had higher calorie intake during the light phase but not during the dark phase, while mice had the same calorie intake per day as controls. DIO rats and mice had lower energy expenditure (EE) and respiratory exchange ratio (RER) than controls. GB-rats reached the plateau (15% weight loss) 2 weeks after surgery and had the same calorie intake as sham-operated controls. EE, but not RER, was higher in GB rats than controls during the dark phase. The lean M3KO mice (25% lighter than wild-type (WT) mice at the plateau between 6 and 15 months of age) had the same calorie intake but higher EE, RER and hypothalamic mRNA expression of NPY, AgRP and leptin receptor than WT mice.Conclusion: When body weight gain or loss reached a plateau, the steady-state energy balance was mainly maintained by EE and/or RER rather than calorie intake.
AB - Objective: We wanted to exam the steady-state energy balance by using high-fat diet-induced obese (DIO) rats and mice as models for positive energy balance, and gastric bypassed (GB) rats and gene knockout of muscarinic acetylcholine M3 receptor (M3KO) mice as models for negative energy balance.Methods: One hundred and thirty-two rats and mice were used. Energy balance was measured by a comprehensive laboratory animal monitoring system. Gene expression was analysed by in situ hybridisation in M3KO mice.Results: DIO rats reached the plateau of body weight 28 weeks after starting high-fat diet (25% heavier than controls), whereas DIO mice reached the plateau after 6 weeks (23% heavier than controls). At the plateau, DIO rats had higher calorie intake during the light phase but not during the dark phase, while mice had the same calorie intake per day as controls. DIO rats and mice had lower energy expenditure (EE) and respiratory exchange ratio (RER) than controls. GB-rats reached the plateau (15% weight loss) 2 weeks after surgery and had the same calorie intake as sham-operated controls. EE, but not RER, was higher in GB rats than controls during the dark phase. The lean M3KO mice (25% lighter than wild-type (WT) mice at the plateau between 6 and 15 months of age) had the same calorie intake but higher EE, RER and hypothalamic mRNA expression of NPY, AgRP and leptin receptor than WT mice.Conclusion: When body weight gain or loss reached a plateau, the steady-state energy balance was mainly maintained by EE and/or RER rather than calorie intake.
KW - eating behaviour
KW - high-fat diet
KW - gastric bypass
KW - Muscarinic acetylcholine M3 receptor knockout
KW - mice
KW - rats
U2 - 10.1080/00365521.2016.1267791
DO - 10.1080/00365521.2016.1267791
M3 - Article
VL - 52
SP - 442
EP - 449
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
SN - 1502-7708
IS - 4
ER -