Steady-state energy balance in animal models of obesity and weight loss

Magnus Kringstad Olsen, Helene Johannessen, Nikki Cassie, Perry Barrett, Koji Takeuchi, Bård Kulseng, Duan Chen, Chun-Mei Zhao

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Abstract

Objective: We wanted to exam the steady-state energy balance by using high-fat diet-induced obese (DIO) rats and mice as models for positive energy balance, and gastric bypassed (GB) rats and gene knockout of muscarinic acetylcholine M3 receptor (M3KO) mice as models for negative energy balance.

Methods: One hundred and thirty-two rats and mice were used. Energy balance was measured by a comprehensive laboratory animal monitoring system. Gene expression was analysed by in situ hybridisation in M3KO mice.

Results: DIO rats reached the plateau of body weight 28 weeks after starting high-fat diet (25% heavier than controls), whereas DIO mice reached the plateau after 6 weeks (23% heavier than controls). At the plateau, DIO rats had higher calorie intake during the light phase but not during the dark phase, while mice had the same calorie intake per day as controls. DIO rats and mice had lower energy expenditure (EE) and respiratory exchange ratio (RER) than controls. GB-rats reached the plateau (15% weight loss) 2 weeks after surgery and had the same calorie intake as sham-operated controls. EE, but not RER, was higher in GB rats than controls during the dark phase. The lean M3KO mice (25% lighter than wild-type (WT) mice at the plateau between 6 and 15 months of age) had the same calorie intake but higher EE, RER and hypothalamic mRNA expression of NPY, AgRP and leptin receptor than WT mice.

Conclusion: When body weight gain or loss reached a plateau, the steady-state energy balance was mainly maintained by EE and/or RER rather than calorie intake.
Original languageEnglish
Pages (from-to)442-449
Number of pages8
JournalScandinavian Journal of Gastroenterology
Volume52
Issue number4
Early online date20 Dec 2016
DOIs
Publication statusPublished - 2017

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Weight Loss
Animal Models
Obesity
Obese Mice
Energy Metabolism
Diet
Stomach
High Fat Diet
Body Weight
Muscarinic M3 Receptors
Gene Knockout Techniques
Laboratory Animals
Muscarinic Receptors
Weight Gain
In Situ Hybridization
Gene Expression
Light
Messenger RNA

Keywords

  • eating behaviour
  • high-fat diet
  • gastric bypass
  • Muscarinic acetylcholine M3 receptor knockout
  • mice
  • rats

Cite this

Olsen, M. K., Johannessen, H., Cassie, N., Barrett, P., Takeuchi, K., Kulseng, B., ... Zhao, C-M. (2017). Steady-state energy balance in animal models of obesity and weight loss. Scandinavian Journal of Gastroenterology, 52(4), 442-449. https://doi.org/10.1080/00365521.2016.1267791

Steady-state energy balance in animal models of obesity and weight loss. / Olsen, Magnus Kringstad ; Johannessen, Helene; Cassie, Nikki; Barrett, Perry; Takeuchi, Koji ; Kulseng, Bård; Chen, Duan; Zhao, Chun-Mei .

In: Scandinavian Journal of Gastroenterology, Vol. 52, No. 4, 2017, p. 442-449.

Research output: Contribution to journalArticle

Olsen, MK, Johannessen, H, Cassie, N, Barrett, P, Takeuchi, K, Kulseng, B, Chen, D & Zhao, C-M 2017, 'Steady-state energy balance in animal models of obesity and weight loss', Scandinavian Journal of Gastroenterology, vol. 52, no. 4, pp. 442-449. https://doi.org/10.1080/00365521.2016.1267791
Olsen, Magnus Kringstad ; Johannessen, Helene ; Cassie, Nikki ; Barrett, Perry ; Takeuchi, Koji ; Kulseng, Bård ; Chen, Duan ; Zhao, Chun-Mei . / Steady-state energy balance in animal models of obesity and weight loss. In: Scandinavian Journal of Gastroenterology. 2017 ; Vol. 52, No. 4. pp. 442-449.
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title = "Steady-state energy balance in animal models of obesity and weight loss",
abstract = "Objective: We wanted to exam the steady-state energy balance by using high-fat diet-induced obese (DIO) rats and mice as models for positive energy balance, and gastric bypassed (GB) rats and gene knockout of muscarinic acetylcholine M3 receptor (M3KO) mice as models for negative energy balance.Methods: One hundred and thirty-two rats and mice were used. Energy balance was measured by a comprehensive laboratory animal monitoring system. Gene expression was analysed by in situ hybridisation in M3KO mice.Results: DIO rats reached the plateau of body weight 28 weeks after starting high-fat diet (25{\%} heavier than controls), whereas DIO mice reached the plateau after 6 weeks (23{\%} heavier than controls). At the plateau, DIO rats had higher calorie intake during the light phase but not during the dark phase, while mice had the same calorie intake per day as controls. DIO rats and mice had lower energy expenditure (EE) and respiratory exchange ratio (RER) than controls. GB-rats reached the plateau (15{\%} weight loss) 2 weeks after surgery and had the same calorie intake as sham-operated controls. EE, but not RER, was higher in GB rats than controls during the dark phase. The lean M3KO mice (25{\%} lighter than wild-type (WT) mice at the plateau between 6 and 15 months of age) had the same calorie intake but higher EE, RER and hypothalamic mRNA expression of NPY, AgRP and leptin receptor than WT mice.Conclusion: When body weight gain or loss reached a plateau, the steady-state energy balance was mainly maintained by EE and/or RER rather than calorie intake.",
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author = "Olsen, {Magnus Kringstad} and Helene Johannessen and Nikki Cassie and Perry Barrett and Koji Takeuchi and B{\aa}rd Kulseng and Duan Chen and Chun-Mei Zhao",
note = "Supported by European Union Seventh Framework Programme (FP7/2007-2013, n°266408 (Full4Health)), the Joint Programme of the Medical Faculty of Norwegian University of Science and Technology (NTNU) and St. Olav’s University Hospital, the Liaison Committee between the Central Norway Regional Health Authority and NTNU.",
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T1 - Steady-state energy balance in animal models of obesity and weight loss

AU - Olsen, Magnus Kringstad

AU - Johannessen, Helene

AU - Cassie, Nikki

AU - Barrett, Perry

AU - Takeuchi, Koji

AU - Kulseng, Bård

AU - Chen, Duan

AU - Zhao, Chun-Mei

N1 - Supported by European Union Seventh Framework Programme (FP7/2007-2013, n°266408 (Full4Health)), the Joint Programme of the Medical Faculty of Norwegian University of Science and Technology (NTNU) and St. Olav’s University Hospital, the Liaison Committee between the Central Norway Regional Health Authority and NTNU.

PY - 2017

Y1 - 2017

N2 - Objective: We wanted to exam the steady-state energy balance by using high-fat diet-induced obese (DIO) rats and mice as models for positive energy balance, and gastric bypassed (GB) rats and gene knockout of muscarinic acetylcholine M3 receptor (M3KO) mice as models for negative energy balance.Methods: One hundred and thirty-two rats and mice were used. Energy balance was measured by a comprehensive laboratory animal monitoring system. Gene expression was analysed by in situ hybridisation in M3KO mice.Results: DIO rats reached the plateau of body weight 28 weeks after starting high-fat diet (25% heavier than controls), whereas DIO mice reached the plateau after 6 weeks (23% heavier than controls). At the plateau, DIO rats had higher calorie intake during the light phase but not during the dark phase, while mice had the same calorie intake per day as controls. DIO rats and mice had lower energy expenditure (EE) and respiratory exchange ratio (RER) than controls. GB-rats reached the plateau (15% weight loss) 2 weeks after surgery and had the same calorie intake as sham-operated controls. EE, but not RER, was higher in GB rats than controls during the dark phase. The lean M3KO mice (25% lighter than wild-type (WT) mice at the plateau between 6 and 15 months of age) had the same calorie intake but higher EE, RER and hypothalamic mRNA expression of NPY, AgRP and leptin receptor than WT mice.Conclusion: When body weight gain or loss reached a plateau, the steady-state energy balance was mainly maintained by EE and/or RER rather than calorie intake.

AB - Objective: We wanted to exam the steady-state energy balance by using high-fat diet-induced obese (DIO) rats and mice as models for positive energy balance, and gastric bypassed (GB) rats and gene knockout of muscarinic acetylcholine M3 receptor (M3KO) mice as models for negative energy balance.Methods: One hundred and thirty-two rats and mice were used. Energy balance was measured by a comprehensive laboratory animal monitoring system. Gene expression was analysed by in situ hybridisation in M3KO mice.Results: DIO rats reached the plateau of body weight 28 weeks after starting high-fat diet (25% heavier than controls), whereas DIO mice reached the plateau after 6 weeks (23% heavier than controls). At the plateau, DIO rats had higher calorie intake during the light phase but not during the dark phase, while mice had the same calorie intake per day as controls. DIO rats and mice had lower energy expenditure (EE) and respiratory exchange ratio (RER) than controls. GB-rats reached the plateau (15% weight loss) 2 weeks after surgery and had the same calorie intake as sham-operated controls. EE, but not RER, was higher in GB rats than controls during the dark phase. The lean M3KO mice (25% lighter than wild-type (WT) mice at the plateau between 6 and 15 months of age) had the same calorie intake but higher EE, RER and hypothalamic mRNA expression of NPY, AgRP and leptin receptor than WT mice.Conclusion: When body weight gain or loss reached a plateau, the steady-state energy balance was mainly maintained by EE and/or RER rather than calorie intake.

KW - eating behaviour

KW - high-fat diet

KW - gastric bypass

KW - Muscarinic acetylcholine M3 receptor knockout

KW - mice

KW - rats

U2 - 10.1080/00365521.2016.1267791

DO - 10.1080/00365521.2016.1267791

M3 - Article

VL - 52

SP - 442

EP - 449

JO - Scandinavian Journal of Gastroenterology

JF - Scandinavian Journal of Gastroenterology

SN - 1502-7708

IS - 4

ER -