Stem-cell-like embryonic explants to study cardiac development

Boni A Afouda

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Cells from the animal pole of Xenopus blastula embryo possess pluripotency that can be used to generate various tissues and even functional organs ex vivo. This finding has sparkled development of a variety of experimental protocols to study mechanisms that underlie formation of various organs and explore strategies for organ engineering for clinical applications. In this chapter, key methods are described for using Xenopus stem-cell-like embryonic explants as an assay system for studying organ development, with a focus on cardiogenesis. This assay allows investigation of cardiogenesis in isolation from neighboring tissues, minimizes interference with other developmental processes, and presents the further advantage of a heterologous system to study cardiogenesis in isolation of endogenous development of the heart. The cardiogenic assays can be exploited to investigate molecular mechanisms and cellular processes that underlie function of different molecules involved in cardiogenesis.
Original languageEnglish
Title of host publicationXenopus Protocols
Subtitle of host publicationPost-Genomic Approaches
EditorsStefan Hoppler, Peter D Vize
PublisherHumana Press
Pages515-523
Number of pages9
ISBN (Electronic)978-1-61779-992-1
ISBN (Print)978-1-61779-991-4
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume917
ISSN (Print)1064-3745

Fingerprint

Embryonic Stem Cells
Xenopus
Biomedical Engineering
Blastula
Embryonic Structures

Cite this

Afouda, B. A. (2012). Stem-cell-like embryonic explants to study cardiac development. In S. Hoppler, & P. D. Vize (Eds.), Xenopus Protocols: Post-Genomic Approaches (pp. 515-523). [917] (Methods in Molecular Biology; Vol. 917). Humana Press. https://doi.org/10.1007/978-1-61779-992-1_29

Stem-cell-like embryonic explants to study cardiac development. / Afouda, Boni A.

Xenopus Protocols: Post-Genomic Approaches. ed. / Stefan Hoppler; Peter D Vize. Humana Press, 2012. p. 515-523 917 (Methods in Molecular Biology; Vol. 917).

Research output: Chapter in Book/Report/Conference proceedingChapter

Afouda, BA 2012, Stem-cell-like embryonic explants to study cardiac development. in S Hoppler & PD Vize (eds), Xenopus Protocols: Post-Genomic Approaches., 917, Methods in Molecular Biology, vol. 917, Humana Press, pp. 515-523. https://doi.org/10.1007/978-1-61779-992-1_29
Afouda BA. Stem-cell-like embryonic explants to study cardiac development. In Hoppler S, Vize PD, editors, Xenopus Protocols: Post-Genomic Approaches. Humana Press. 2012. p. 515-523. 917. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-992-1_29
Afouda, Boni A. / Stem-cell-like embryonic explants to study cardiac development. Xenopus Protocols: Post-Genomic Approaches. editor / Stefan Hoppler ; Peter D Vize. Humana Press, 2012. pp. 515-523 (Methods in Molecular Biology).
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