Storage of Dothistroma septosporum cultures

S. Fraser, H. J. Weitz, A. V. Brown, S. Woodward

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Culture collections are invaluable resources for supporting current and future research activities on plant pathogens. Reliable culture storage (preservation) methods are required for these collections. To determine what methods of storage were most reliable for the pine-needle pathogen Dothistroma septosporum, six widely available methods for the storage of fungi were compared: agar slopes at 4°C; slopes at −20°C, mycelial plugs under sterile reverse osmosis (RO) water at 4°C, mycelial plugs under sterile mineral oil at 4°C, uncovered mycelial plugs at 4°C and mycelial plugs under sterile 10% glycerol at −80°C. Revival of cultures was assessed after 12 and 21 months. Storage of mycelial plugs under sterile RO water at 4°C and under glycerol at −80°C both had 100% culture revival at both time points and are therefore recommended for future use. The four remaining methods had revival rates below 70% at 12 months.

Original languageEnglish
Pages (from-to)547-550
Number of pages4
JournalForest Pathology
Volume46
Issue number5
Early online date25 Mar 2015
DOIs
Publication statusPublished - Oct 2016

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Dothistroma
reverse osmosis
glycerol
pathogen
conifer needles
mineral oil
plant pathogens
agar
water
fungus
fungi
method
pathogens
resource
methodology

ASJC Scopus subject areas

  • Forestry
  • Ecology

Cite this

Storage of Dothistroma septosporum cultures. / Fraser, S.; Weitz, H. J.; Brown, A. V.; Woodward, S.

In: Forest Pathology, Vol. 46, No. 5, 10.2016, p. 547-550.

Research output: Contribution to journalArticle

Fraser, S, Weitz, HJ, Brown, AV & Woodward, S 2016, 'Storage of Dothistroma septosporum cultures' Forest Pathology, vol. 46, no. 5, pp. 547-550. https://doi.org/10.1111/efp.12189
Fraser, S. ; Weitz, H. J. ; Brown, A. V. ; Woodward, S. / Storage of Dothistroma septosporum cultures. In: Forest Pathology. 2016 ; Vol. 46, No. 5. pp. 547-550.
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