Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus

Faizah N. H. H. Kh. Alenezi, Imen Rekik, Marta Belka, Abrar F. Ibrahim, Lenka Luptakova, Marcel Jaspars, Stephen Woodward, Lassaad Belbahri*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin S (GS), by the two strains, is not the sole mechanism of inhibition. The availability of four sequenced genomes of Aneurinibacillus prompted us to apply genome mining techniques to identify the bioactive potential of A. migulanus and to provide insights into the secondary metabolite arsenal of the genus Aneurinibacillus. Up to eleven secondary metabolite biosynthetic gene clusters were present in the three Aneurinibacillus species. Biosynthetic gene clusters specifying bacteriocins, microcins, non-ribosomal peptides, polyketides, terpenes, phosphonates, lasso peptides and linaridins were identified. Chitinolytic potential and iron metabolism regulation were also investigated. With increasing numbers of biocontrol bacterial genomes being sequenced and mined, the use of approaches similar to those described in this paper will lead to an increase in the numbers of environmentally friendly natural products available to use against plant diseases. (c) 2015 Elsevier GmbH. All rights reserved.

Original languageEnglish
Pages (from-to)116-124
Number of pages9
JournalMicrobiological Research
Volume182
Early online date28 Oct 2015
DOIs
Publication statusPublished - Jan 2016

Keywords

  • Secondary metabolism
  • Bioinformatics
  • Genome mining
  • Aneurinibacillus migulanus
  • Biocontrol bacteria
  • Brevibacillus-brevis
  • Bacterial chitinases
  • Genome sequence
  • Gene clusters
  • Identification

Cite this

Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus. / Alenezi, Faizah N. H. H. Kh.; Rekik, Imen; Belka, Marta; Ibrahim, Abrar F.; Luptakova, Lenka; Jaspars, Marcel; Woodward, Stephen; Belbahri, Lassaad.

In: Microbiological Research, Vol. 182, 01.2016, p. 116-124.

Research output: Contribution to journalArticle

Alenezi, Faizah N. H. H. Kh. ; Rekik, Imen ; Belka, Marta ; Ibrahim, Abrar F. ; Luptakova, Lenka ; Jaspars, Marcel ; Woodward, Stephen ; Belbahri, Lassaad. / Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus. In: Microbiological Research. 2016 ; Vol. 182. pp. 116-124.
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abstract = "Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin S (GS), by the two strains, is not the sole mechanism of inhibition. The availability of four sequenced genomes of Aneurinibacillus prompted us to apply genome mining techniques to identify the bioactive potential of A. migulanus and to provide insights into the secondary metabolite arsenal of the genus Aneurinibacillus. Up to eleven secondary metabolite biosynthetic gene clusters were present in the three Aneurinibacillus species. Biosynthetic gene clusters specifying bacteriocins, microcins, non-ribosomal peptides, polyketides, terpenes, phosphonates, lasso peptides and linaridins were identified. Chitinolytic potential and iron metabolism regulation were also investigated. With increasing numbers of biocontrol bacterial genomes being sequenced and mined, the use of approaches similar to those described in this paper will lead to an increase in the numbers of environmentally friendly natural products available to use against plant diseases. (c) 2015 Elsevier GmbH. All rights reserved.",
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AU - Jaspars, Marcel

AU - Woodward, Stephen

AU - Belbahri, Lassaad

N1 - Funding This project was funded by the Government of Kuwait (to F.N.A.) and the European Union’s Seventh Framework Programme under grant agreement 245268 (ISEFOR; to L.B. and S.W.). Further support came from the SwissBOL project, financed by the Swiss Federal Office for the Environment (grant holder L.B.) and the Sciex–Scientific Exchange Programme NMS.CH (to L.L. and L.B.).

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N2 - Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin S (GS), by the two strains, is not the sole mechanism of inhibition. The availability of four sequenced genomes of Aneurinibacillus prompted us to apply genome mining techniques to identify the bioactive potential of A. migulanus and to provide insights into the secondary metabolite arsenal of the genus Aneurinibacillus. Up to eleven secondary metabolite biosynthetic gene clusters were present in the three Aneurinibacillus species. Biosynthetic gene clusters specifying bacteriocins, microcins, non-ribosomal peptides, polyketides, terpenes, phosphonates, lasso peptides and linaridins were identified. Chitinolytic potential and iron metabolism regulation were also investigated. With increasing numbers of biocontrol bacterial genomes being sequenced and mined, the use of approaches similar to those described in this paper will lead to an increase in the numbers of environmentally friendly natural products available to use against plant diseases. (c) 2015 Elsevier GmbH. All rights reserved.

AB - Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin S (GS), by the two strains, is not the sole mechanism of inhibition. The availability of four sequenced genomes of Aneurinibacillus prompted us to apply genome mining techniques to identify the bioactive potential of A. migulanus and to provide insights into the secondary metabolite arsenal of the genus Aneurinibacillus. Up to eleven secondary metabolite biosynthetic gene clusters were present in the three Aneurinibacillus species. Biosynthetic gene clusters specifying bacteriocins, microcins, non-ribosomal peptides, polyketides, terpenes, phosphonates, lasso peptides and linaridins were identified. Chitinolytic potential and iron metabolism regulation were also investigated. With increasing numbers of biocontrol bacterial genomes being sequenced and mined, the use of approaches similar to those described in this paper will lead to an increase in the numbers of environmentally friendly natural products available to use against plant diseases. (c) 2015 Elsevier GmbH. All rights reserved.

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KW - Identification

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