Abstract
Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin S (GS), by the two strains, is not the sole mechanism of inhibition. The availability of four sequenced genomes of Aneurinibacillus prompted us to apply genome mining techniques to identify the bioactive potential of A. migulanus and to provide insights into the secondary metabolite arsenal of the genus Aneurinibacillus. Up to eleven secondary metabolite biosynthetic gene clusters were present in the three Aneurinibacillus species. Biosynthetic gene clusters specifying bacteriocins, microcins, non-ribosomal peptides, polyketides, terpenes, phosphonates, lasso peptides and linaridins were identified. Chitinolytic potential and iron metabolism regulation were also investigated. With increasing numbers of biocontrol bacterial genomes being sequenced and mined, the use of approaches similar to those described in this paper will lead to an increase in the numbers of environmentally friendly natural products available to use against plant diseases. (c) 2015 Elsevier GmbH. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 116-124 |
| Number of pages | 9 |
| Journal | Microbiological Research |
| Volume | 182 |
| Early online date | 28 Oct 2015 |
| DOIs | |
| Publication status | Published - Jan 2016 |
Keywords
- Secondary metabolism
- Bioinformatics
- Genome mining
- Aneurinibacillus migulanus
- Biocontrol bacteria
- Brevibacillus-brevis
- Bacterial chitinases
- Genome sequence
- Gene clusters
- Identification
Cite this
Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus. / Alenezi, Faizah N. H. H. Kh.; Rekik, Imen; Belka, Marta; Ibrahim, Abrar F.; Luptakova, Lenka; Jaspars, Marcel; Woodward, Stephen; Belbahri, Lassaad.
In: Microbiological Research, Vol. 182, 01.2016, p. 116-124.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus
AU - Alenezi, Faizah N. H. H. Kh.
AU - Rekik, Imen
AU - Belka, Marta
AU - Ibrahim, Abrar F.
AU - Luptakova, Lenka
AU - Jaspars, Marcel
AU - Woodward, Stephen
AU - Belbahri, Lassaad
N1 - Funding This project was funded by the Government of Kuwait (to F.N.A.) and the European Union’s Seventh Framework Programme under grant agreement 245268 (ISEFOR; to L.B. and S.W.). Further support came from the SwissBOL project, financed by the Swiss Federal Office for the Environment (grant holder L.B.) and the Sciex–Scientific Exchange Programme NMS.CH (to L.L. and L.B.).
PY - 2016/1
Y1 - 2016/1
N2 - Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin S (GS), by the two strains, is not the sole mechanism of inhibition. The availability of four sequenced genomes of Aneurinibacillus prompted us to apply genome mining techniques to identify the bioactive potential of A. migulanus and to provide insights into the secondary metabolite arsenal of the genus Aneurinibacillus. Up to eleven secondary metabolite biosynthetic gene clusters were present in the three Aneurinibacillus species. Biosynthetic gene clusters specifying bacteriocins, microcins, non-ribosomal peptides, polyketides, terpenes, phosphonates, lasso peptides and linaridins were identified. Chitinolytic potential and iron metabolism regulation were also investigated. With increasing numbers of biocontrol bacterial genomes being sequenced and mined, the use of approaches similar to those described in this paper will lead to an increase in the numbers of environmentally friendly natural products available to use against plant diseases. (c) 2015 Elsevier GmbH. All rights reserved.
AB - Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin S (GS), by the two strains, is not the sole mechanism of inhibition. The availability of four sequenced genomes of Aneurinibacillus prompted us to apply genome mining techniques to identify the bioactive potential of A. migulanus and to provide insights into the secondary metabolite arsenal of the genus Aneurinibacillus. Up to eleven secondary metabolite biosynthetic gene clusters were present in the three Aneurinibacillus species. Biosynthetic gene clusters specifying bacteriocins, microcins, non-ribosomal peptides, polyketides, terpenes, phosphonates, lasso peptides and linaridins were identified. Chitinolytic potential and iron metabolism regulation were also investigated. With increasing numbers of biocontrol bacterial genomes being sequenced and mined, the use of approaches similar to those described in this paper will lead to an increase in the numbers of environmentally friendly natural products available to use against plant diseases. (c) 2015 Elsevier GmbH. All rights reserved.
KW - Secondary metabolism
KW - Bioinformatics
KW - Genome mining
KW - Aneurinibacillus migulanus
KW - Biocontrol bacteria
KW - Brevibacillus-brevis
KW - Bacterial chitinases
KW - Genome sequence
KW - Gene clusters
KW - Identification
U2 - 10.1016/j.micres.2015.10.007
DO - 10.1016/j.micres.2015.10.007
M3 - Article
VL - 182
SP - 116
EP - 124
JO - Microbiological Research
JF - Microbiological Research
SN - 0944-5013
ER -