Strain Typing and Determination of Population Structure of Candida krusei by Multilocus Sequence Typing

Mette Dorph Jacobsen, Neil Andrew Robert Gow, Martin C. J. Maiden, Duncan James Shaw, Frank Christopher Odds

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A multilocus sequence typing (MLST) scheme for Candida krusei was devised, based on sequencing of six gene fragments of the species. The existence of heterozygous results for each of the six fragments sequenced confirms that C krusei is diploid for at least part of its genome. The C krusei MLST scheme had a discriminatory index of 0.998, making this system ideal for strain typing of C. krusei clinical isolates. MLST data for 122 independent C. krusei isolates from a range of geographical sources were analyzed by eBURST, structure, and the unweighted-pair group method using average linkages to derive a population structure comprising four subtype strain clusters. There was no evidence of geographical associations with particular subtypes. Data for pairs of isolates from seven patients showed that each patient was colonized and/or infected with strain types that were indistinguishable by MLST. The C krusei MLST database can be accessed online at http://pubmlst.org/ckrusei.

Original languageEnglish
Pages (from-to)317-323
Number of pages7
JournalJournal of Clinical Microbiology
Volume45
Issue number2
DOIs
Publication statusPublished - Feb 2007

Keywords

  • blood-stream infections
  • in-vitro activities
  • immunocompromised patients
  • antifungal susceptibility
  • United-States
  • genotype data
  • albicans
  • fluconazole
  • fungemia
  • surveillance

Cite this

Strain Typing and Determination of Population Structure of Candida krusei by Multilocus Sequence Typing. / Jacobsen, Mette Dorph; Gow, Neil Andrew Robert; Maiden, Martin C. J.; Shaw, Duncan James; Odds, Frank Christopher.

In: Journal of Clinical Microbiology, Vol. 45, No. 2, 02.2007, p. 317-323.

Research output: Contribution to journalArticle

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