Stress adaptation in a pathogenic fungus

Alistair J P Brown, Susan Budge, Despoina Kaloriti, Anna Tillmann, Mette D Jacobsen, Zhikang Yin, Iuliana V Ene, Iryna Bohovych, Doblin Sandai, Stavroula Kastora, Joanna Potrykus, Elizabeth R Ballou, Delma S Childers, Shahida Shahana, Michelle D Leach

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Abstract

Candida albicans is a major fungal pathogen of humans. This yeast is carried by many individuals as a harmless commensal, but when immune defences are perturbed it causes mucosal infections (thrush). Additionally, when the immune system becomes severely compromised, C. albicans often causes life-threatening systemic infections. A battery of virulence factors and fitness attributes promote the pathogenicity of C. albicans. Fitness attributes include robust responses to local environmental stresses, the inactivation of which attenuates virulence. Stress signalling pathways in C. albicans include evolutionarily conserved modules. However, there has been rewiring of some stress regulatory circuitry such that the roles of a number of regulators in C. albicans have diverged relative to the benign model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This reflects the specific evolution of C. albicans as an opportunistic pathogen obligately associated with warm-blooded animals, compared with other yeasts that are found across diverse environmental niches. Our understanding of C. albicans stress signalling is based primarily on the in vitro responses of glucose-grown cells to individual stresses. However, in vivo this pathogen occupies complex and dynamic host niches characterised by alternative carbon sources and simultaneous exposure to combinations of stresses (rather than individual stresses). It has become apparent that changes in carbon source strongly influence stress resistance, and that some combinatorial stresses exert non-additive effects upon C. albicans. These effects, which are relevant to fungus-host interactions during disease progression, are mediated by multiple mechanisms that include signalling and chemical crosstalk, stress pathway interference and a biological transistor.
Original languageEnglish
Pages (from-to)144-155
Number of pages12
JournalJournal of Experimental Biology
Volume217
Issue number1
DOIs
Publication statusPublished - 1 Jan 2014

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Candida albicans
Fungi
fungus
fungi
yeast
pathogen
Yeasts
yeasts
virulence
Virulence
pathogens
niche
niches
fitness
Carbon
Oral Candidiasis
stress resistance
commensal
Schizosaccharomyces
carbon

Keywords

  • candida albicans
  • fungal pathogenicity
  • heat shock
  • oxidative stress
  • nitrosative stress
  • osmotic stress
  • cationic stress
  • stress adaptation
  • carbon metabolism

Cite this

Brown, A. J. P., Budge, S., Kaloriti, D., Tillmann, A., Jacobsen, M. D., Yin, Z., ... Leach, M. D. (2014). Stress adaptation in a pathogenic fungus. Journal of Experimental Biology, 217(1), 144-155. https://doi.org/10.1242/jeb.088930

Stress adaptation in a pathogenic fungus. / Brown, Alistair J P; Budge, Susan; Kaloriti, Despoina; Tillmann, Anna; Jacobsen, Mette D; Yin, Zhikang; Ene, Iuliana V; Bohovych, Iryna; Sandai, Doblin; Kastora, Stavroula; Potrykus, Joanna; Ballou, Elizabeth R; Childers, Delma S; Shahana, Shahida; Leach, Michelle D.

In: Journal of Experimental Biology, Vol. 217, No. 1, 01.01.2014, p. 144-155.

Research output: Contribution to journalArticle

Brown, AJP, Budge, S, Kaloriti, D, Tillmann, A, Jacobsen, MD, Yin, Z, Ene, IV, Bohovych, I, Sandai, D, Kastora, S, Potrykus, J, Ballou, ER, Childers, DS, Shahana, S & Leach, MD 2014, 'Stress adaptation in a pathogenic fungus', Journal of Experimental Biology, vol. 217, no. 1, pp. 144-155. https://doi.org/10.1242/jeb.088930
Brown AJP, Budge S, Kaloriti D, Tillmann A, Jacobsen MD, Yin Z et al. Stress adaptation in a pathogenic fungus. Journal of Experimental Biology. 2014 Jan 1;217(1):144-155. https://doi.org/10.1242/jeb.088930
Brown, Alistair J P ; Budge, Susan ; Kaloriti, Despoina ; Tillmann, Anna ; Jacobsen, Mette D ; Yin, Zhikang ; Ene, Iuliana V ; Bohovych, Iryna ; Sandai, Doblin ; Kastora, Stavroula ; Potrykus, Joanna ; Ballou, Elizabeth R ; Childers, Delma S ; Shahana, Shahida ; Leach, Michelle D. / Stress adaptation in a pathogenic fungus. In: Journal of Experimental Biology. 2014 ; Vol. 217, No. 1. pp. 144-155.
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abstract = "Candida albicans is a major fungal pathogen of humans. This yeast is carried by many individuals as a harmless commensal, but when immune defences are perturbed it causes mucosal infections (thrush). Additionally, when the immune system becomes severely compromised, C. albicans often causes life-threatening systemic infections. A battery of virulence factors and fitness attributes promote the pathogenicity of C. albicans. Fitness attributes include robust responses to local environmental stresses, the inactivation of which attenuates virulence. Stress signalling pathways in C. albicans include evolutionarily conserved modules. However, there has been rewiring of some stress regulatory circuitry such that the roles of a number of regulators in C. albicans have diverged relative to the benign model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This reflects the specific evolution of C. albicans as an opportunistic pathogen obligately associated with warm-blooded animals, compared with other yeasts that are found across diverse environmental niches. Our understanding of C. albicans stress signalling is based primarily on the in vitro responses of glucose-grown cells to individual stresses. However, in vivo this pathogen occupies complex and dynamic host niches characterised by alternative carbon sources and simultaneous exposure to combinations of stresses (rather than individual stresses). It has become apparent that changes in carbon source strongly influence stress resistance, and that some combinatorial stresses exert non-additive effects upon C. albicans. These effects, which are relevant to fungus-host interactions during disease progression, are mediated by multiple mechanisms that include signalling and chemical crosstalk, stress pathway interference and a biological transistor.",
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