Structure of forked DNA in solution revealed by high-resolution single-molecule FRET

Tara Sabir, Gunnar Schroder, Anita Toulmin, Peter McGlynn, Steven Magennis

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Branched DNA structures play critical roles in
DNA replication, repair, and recombination in addition to
being key building blocks for DNA nanotechnology. Here we
combine single-molecule multiparameter fluorescence detection
and molecular dynamics simulations to give a general
approach to global structure determination of branched DNA
in solution. We reveal an open, planar structure of a forked
DNA molecule with three duplex arms and demonstrate an
ion-induced conformational change. This structure will serve
as a benchmark for DNA-protein interaction studies.
Original languageEnglish
Pages (from-to)1188-1191
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Publication statusPublished - 2011

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