Structure of forked DNA in solution revealed by high-resolution single-molecule FRET

Tara Sabir, Gunnar Schroder, Anita Toulmin, Peter McGlynn, Steven Magennis

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Branched DNA structures play critical roles in
DNA replication, repair, and recombination in addition to
being key building blocks for DNA nanotechnology. Here we
combine single-molecule multiparameter fluorescence detection
and molecular dynamics simulations to give a general
approach to global structure determination of branched DNA
in solution. We reveal an open, planar structure of a forked
DNA molecule with three duplex arms and demonstrate an
ion-induced conformational change. This structure will serve
as a benchmark for DNA-protein interaction studies.
Original languageEnglish
Pages (from-to)1188-1191
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Publication statusPublished - 2011

Fingerprint

DNA
Molecules
Recombinational DNA Repair
Benchmarking
Nanotechnology
Molecular Dynamics Simulation
Anions
Molecular dynamics
Repair
Negative ions
Fluorescence
Proteins
Computer simulation

Cite this

Sabir, T., Schroder, G., Toulmin, A., McGlynn, P., & Magennis, S. (2011). Structure of forked DNA in solution revealed by high-resolution single-molecule FRET. Journal of the American Chemical Society, 133, 1188-1191.

Structure of forked DNA in solution revealed by high-resolution single-molecule FRET. / Sabir, Tara; Schroder, Gunnar; Toulmin, Anita; McGlynn, Peter; Magennis, Steven.

In: Journal of the American Chemical Society, Vol. 133, 2011, p. 1188-1191.

Research output: Contribution to journalArticle

Sabir, T, Schroder, G, Toulmin, A, McGlynn, P & Magennis, S 2011, 'Structure of forked DNA in solution revealed by high-resolution single-molecule FRET', Journal of the American Chemical Society, vol. 133, pp. 1188-1191.
Sabir, Tara ; Schroder, Gunnar ; Toulmin, Anita ; McGlynn, Peter ; Magennis, Steven. / Structure of forked DNA in solution revealed by high-resolution single-molecule FRET. In: Journal of the American Chemical Society. 2011 ; Vol. 133. pp. 1188-1191.
@article{492c2c70b5c44296b57137c3556deb23,
title = "Structure of forked DNA in solution revealed by high-resolution single-molecule FRET",
abstract = "Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA-protein interaction studies.",
author = "Tara Sabir and Gunnar Schroder and Anita Toulmin and Peter McGlynn and Steven Magennis",
year = "2011",
language = "English",
volume = "133",
pages = "1188--1191",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",

}

TY - JOUR

T1 - Structure of forked DNA in solution revealed by high-resolution single-molecule FRET

AU - Sabir, Tara

AU - Schroder, Gunnar

AU - Toulmin, Anita

AU - McGlynn, Peter

AU - Magennis, Steven

PY - 2011

Y1 - 2011

N2 - Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA-protein interaction studies.

AB - Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA-protein interaction studies.

M3 - Article

VL - 133

SP - 1188

EP - 1191

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

ER -