Structure of PatF from Prochloron didemni

A. F. Bent, J. Koehnke, W. E. Houssen, M. C. M. Smith, M. Jaspars, J.H. Naismith

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Patellamides are macrocyclic peptides with potent biological effects and are a subset of the cyanobactins. Cyanobactins are natural products that are produced by a series of enzymatic transformations and a common modification is the addition of a prenyl group. Puzzlingly, the pathway for patellamides in Prochloron didemni contains a gene, patF, with homology to prenylases, but patellamides are not themselves prenylated. The structure of the protein PatF was cloned, expressed, purified and determined. Prenylase activity could not be demonstrated for the protein, and examination of the structure revealed changes in side-chain identity at the active site. It is suggested that these changes have inactivated the protein. Attempts to mutate these residues led to unfolded protein.
Original languageEnglish
Pages (from-to)618-623
Number of pages6
JournalActa Crystallographica Section F: Structural Biology Communications
Volume69
Issue number6
DOIs
Publication statusPublished - Jun 2013

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Prochloron
proteins
Protein Unfolding
Proteins
Biological Products
biological effects
Catalytic Domain
homology
genes
set theory
peptides
Peptides
Genes
examination
products

Keywords

  • patellamide
  • cyanobactins
  • natural products
  • prenyltransferases

Cite this

Structure of PatF from Prochloron didemni. / Bent, A. F.; Koehnke, J. ; Houssen, W. E.; Smith, M. C. M.; Jaspars, M.; Naismith, J.H.

In: Acta Crystallographica Section F: Structural Biology Communications , Vol. 69, No. 6, 06.2013, p. 618-623.

Research output: Contribution to journalArticle

Bent, A. F. ; Koehnke, J. ; Houssen, W. E. ; Smith, M. C. M. ; Jaspars, M. ; Naismith, J.H. / Structure of PatF from Prochloron didemni. In: Acta Crystallographica Section F: Structural Biology Communications . 2013 ; Vol. 69, No. 6. pp. 618-623.
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