Subcellular localization of phosphatidylinositol 4,5-bisphosphate using the pleckstrin homology domain of phospholipase C delta1

Stephen A Watt, Gursant Kular, Ian N Fleming, C Peter Downes, John M Lucocq

Research output: Contribution to journalArticle

282 Citations (Scopus)

Abstract

Ptd(4,5)P(2) is thought to promote and organize a wide range of cellular functions, including vesicular membrane traffic and cytoskeletal dynamics, by recruiting functional protein complexes to restricted locations in cellular membranes. However, little is known about the distribution of PtdIns(4,5)P(2) in the cell at high resolution. We have used the pleckstrin homology (PH) domain of phospholipase delta(1) (PLCdelta(1)), narrowly specific for PtdIns(4,5)P(2), to map the distribution of the lipid in astrocytoma and A431 cells. We applied the glutathione S-transferase-tagged PLCdelta(1) PH domain (PLCdelta(1)PH-GST) in an on-section labelling approach which avoids transfection procedures. Here we demonstrate PtdIns(4,5)P(2) labelling in the plasma membrane, and also in intracellular membranes, including Golgi (mainly stack), endosomes and endoplasmic reticulum, as well as in electron-dense structures within the nucleus. At the plasma membrane, labelling was more concentrated over lamellipodia, but not in caveolae, which contained less than 10% of the total cell-surface labelling. A dramatic decrease in signal over labelled compartments was observed on preincubation with the cognate headgroup [Ins(1,4,5)P(3)], and plasma-membrane labelling was substantially decreased after stimulation with thrombin-receptor-activating peptide (SFLLRN in the one-letter amino acid code), a treatment which markedly diminishes PtdIns(4,5)P(2) levels. Thus we have developed a highly selective method for mapping the PtdIns(4,5)P(2) distribution within cells at high resolution, and our data provide direct evidence for this lipid at key functional locations.
Original languageEnglish
Pages (from-to)657-66
Number of pages10
JournalBiochemical Journal
Volume363
Issue numberPt 3
Publication statusPublished - 2002

Keywords

  • Blood Proteins
  • Cell Line
  • Cell Membrane
  • Humans
  • Intracellular Membranes
  • Isoenzymes
  • Lipid Metabolism
  • Microscopy, Immunoelectron
  • Peptide Fragments
  • Phosphatidylinositol 4,5-Diphosphate
  • Phospholipase C delta
  • Phosphoproteins
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured
  • Type C Phospholipases

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