Subclinical infection with the nematode Trichostrongylus colubriformis increases gastrointestinal tract leucine metabolism and reduces availability of leucine for other tissues

F Yu, L A Bruce, Alexander Graham Calder, Eric Milne, R L Coop, F Jackson, G W Horgan, J C MacRae

    Research output: Contribution to journalArticle

    63 Citations (Scopus)

    Abstract

    Gastrointestinal (GI) tract leucine metabolism was measured in 6- to 9-mo-old lambs subjected to trickle infection with Trichostrongylus colubriformis larvae and in separate animals that were not infected. Animals prepared with a jejunal catheter and with indwelling catheters into the aorta and the portal- (PDV) and mesenteric- (MDV) drained viscera were infused simultaneously with [1-C-13] and [5,5,5-H-2(3)] leucine to determine GI tract sequestration of leucine from arterial and luminal amino acid pools by tracer and tracee arteriovenous concentration differences. Leucine oxidative losses and net fluxes were also determined across the GI tract. Infection had no detect-able effect on whole-body leucine flux, but it increased total GI tract leucine sequestration by 24% (P <.05) and GI tract oxidative losses of leucine by 22 to 41% (P <.01). Net PDV fluxes of leucine were decreased by 20 to 32% during the infection. The infection did not alter either the proportion of precursor leucine used by GI tract metabolism that was derived from the arterial leucine pool (.84 to .88) or the proportional sequestration of digesta-derived leucine during "first pass" absorptive metabolism (.12 to .18). These findings help to elucidate the metabolic basis for the reduced growth rates and nitrogen retention observed when animals are subjected to subclinical nematode infection.

    Original languageEnglish
    Pages (from-to)380-390
    Number of pages11
    JournalJournal of Animal Science
    Volume78
    Issue number2
    Publication statusPublished - Feb 2000

    Keywords

    • digestive tract
    • amino acids
    • stable isotopes
    • sheep
    • nematoda
    • food-intake
    • protein-synthesis
    • dietary-protein
    • growing lambs
    • amino-acids
    • whole-body
    • consequences
    • catabolism
    • parasitism

    Cite this

    Subclinical infection with the nematode Trichostrongylus colubriformis increases gastrointestinal tract leucine metabolism and reduces availability of leucine for other tissues. / Yu, F ; Bruce, L A ; Calder, Alexander Graham; Milne, Eric; Coop, R L ; Jackson, F ; Horgan, G W ; MacRae, J C .

    In: Journal of Animal Science, Vol. 78, No. 2, 02.2000, p. 380-390.

    Research output: Contribution to journalArticle

    Yu, F ; Bruce, L A ; Calder, Alexander Graham ; Milne, Eric ; Coop, R L ; Jackson, F ; Horgan, G W ; MacRae, J C . / Subclinical infection with the nematode Trichostrongylus colubriformis increases gastrointestinal tract leucine metabolism and reduces availability of leucine for other tissues. In: Journal of Animal Science. 2000 ; Vol. 78, No. 2. pp. 380-390.
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    abstract = "Gastrointestinal (GI) tract leucine metabolism was measured in 6- to 9-mo-old lambs subjected to trickle infection with Trichostrongylus colubriformis larvae and in separate animals that were not infected. Animals prepared with a jejunal catheter and with indwelling catheters into the aorta and the portal- (PDV) and mesenteric- (MDV) drained viscera were infused simultaneously with [1-C-13] and [5,5,5-H-2(3)] leucine to determine GI tract sequestration of leucine from arterial and luminal amino acid pools by tracer and tracee arteriovenous concentration differences. Leucine oxidative losses and net fluxes were also determined across the GI tract. Infection had no detect-able effect on whole-body leucine flux, but it increased total GI tract leucine sequestration by 24{\%} (P <.05) and GI tract oxidative losses of leucine by 22 to 41{\%} (P <.01). Net PDV fluxes of leucine were decreased by 20 to 32{\%} during the infection. The infection did not alter either the proportion of precursor leucine used by GI tract metabolism that was derived from the arterial leucine pool (.84 to .88) or the proportional sequestration of digesta-derived leucine during {"}first pass{"} absorptive metabolism (.12 to .18). These findings help to elucidate the metabolic basis for the reduced growth rates and nitrogen retention observed when animals are subjected to subclinical nematode infection.",
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    T1 - Subclinical infection with the nematode Trichostrongylus colubriformis increases gastrointestinal tract leucine metabolism and reduces availability of leucine for other tissues

    AU - Yu, F

    AU - Bruce, L A

    AU - Calder, Alexander Graham

    AU - Milne, Eric

    AU - Coop, R L

    AU - Jackson, F

    AU - Horgan, G W

    AU - MacRae, J C

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    N2 - Gastrointestinal (GI) tract leucine metabolism was measured in 6- to 9-mo-old lambs subjected to trickle infection with Trichostrongylus colubriformis larvae and in separate animals that were not infected. Animals prepared with a jejunal catheter and with indwelling catheters into the aorta and the portal- (PDV) and mesenteric- (MDV) drained viscera were infused simultaneously with [1-C-13] and [5,5,5-H-2(3)] leucine to determine GI tract sequestration of leucine from arterial and luminal amino acid pools by tracer and tracee arteriovenous concentration differences. Leucine oxidative losses and net fluxes were also determined across the GI tract. Infection had no detect-able effect on whole-body leucine flux, but it increased total GI tract leucine sequestration by 24% (P <.05) and GI tract oxidative losses of leucine by 22 to 41% (P <.01). Net PDV fluxes of leucine were decreased by 20 to 32% during the infection. The infection did not alter either the proportion of precursor leucine used by GI tract metabolism that was derived from the arterial leucine pool (.84 to .88) or the proportional sequestration of digesta-derived leucine during "first pass" absorptive metabolism (.12 to .18). These findings help to elucidate the metabolic basis for the reduced growth rates and nitrogen retention observed when animals are subjected to subclinical nematode infection.

    AB - Gastrointestinal (GI) tract leucine metabolism was measured in 6- to 9-mo-old lambs subjected to trickle infection with Trichostrongylus colubriformis larvae and in separate animals that were not infected. Animals prepared with a jejunal catheter and with indwelling catheters into the aorta and the portal- (PDV) and mesenteric- (MDV) drained viscera were infused simultaneously with [1-C-13] and [5,5,5-H-2(3)] leucine to determine GI tract sequestration of leucine from arterial and luminal amino acid pools by tracer and tracee arteriovenous concentration differences. Leucine oxidative losses and net fluxes were also determined across the GI tract. Infection had no detect-able effect on whole-body leucine flux, but it increased total GI tract leucine sequestration by 24% (P <.05) and GI tract oxidative losses of leucine by 22 to 41% (P <.01). Net PDV fluxes of leucine were decreased by 20 to 32% during the infection. The infection did not alter either the proportion of precursor leucine used by GI tract metabolism that was derived from the arterial leucine pool (.84 to .88) or the proportional sequestration of digesta-derived leucine during "first pass" absorptive metabolism (.12 to .18). These findings help to elucidate the metabolic basis for the reduced growth rates and nitrogen retention observed when animals are subjected to subclinical nematode infection.

    KW - digestive tract

    KW - amino acids

    KW - stable isotopes

    KW - sheep

    KW - nematoda

    KW - food-intake

    KW - protein-synthesis

    KW - dietary-protein

    KW - growing lambs

    KW - amino-acids

    KW - whole-body

    KW - consequences

    KW - catabolism

    KW - parasitism

    M3 - Article

    VL - 78

    SP - 380

    EP - 390

    JO - Journal of Animal Science

    JF - Journal of Animal Science

    SN - 0021-8812

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    ER -