Abstract
Roseburia inulinivorans is a recently identified motile representative of the Firmicutes that contributes to butyrate formation from a variety of dietary polysaccharide substrates in the human large intestine. Microarray analysis was used here to investigate substrate-driven gene-expression changes in R. inulinivorans A2-194. A cluster of fructo-oligosaccharide/inulin utilization genes induced during growth on inulin included one encoding a beta-fructofuranosidase protein that was prominent in the proteome of inulin-grown cells. This cluster also included a 6-phosphofructokinase and an ABC transport system, whereas a distinct inulin-induced 1-phosphofructokinase was linked to a fructose-specific phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS II transport enzyme). Real-time PCR analysis showed that the beta-fructofuranosidase and adjacent ABC transport protein showed greatest induction during growth on inulin, whereas the 1-phosphofructokinase enzyme and linked sugar phosphotransferase transport system were most strongly up-regulated during growth on fructose, indicating that these two clusters play distinct roles in the use of inulin. The R. inulinivorans beta-fructofuranosidase was overexpressed in Escherichia coli and shown to hydrolyze fructans ranging from inulin down to sucrose, with greatest activity on fructo-oligosaccharides. Genes induced on starch included the major extracellular a-amylase and two distinct alpha-glucanotransferases together with a gene encoding a flagellin protein. The latter response may be concerned with improving bacterial access to insoluble starch particles.
Original language | English |
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Pages (from-to) | 4672-4679 |
Number of pages | 8 |
Journal | PNAS |
Volume | 108 |
Issue number | Supplement 1 |
DOIs | |
Publication status | Published - 15 Mar 2011 |
Keywords
- anaerobic gut bacteria
- differential gene expression
- fructo-oligosaccharides
- butyrate
- prebiotic
- human fecal flora
- beta-fructofuranosidase
- human gut
- human colon
- lactobacillus-acidophilus
- bifidobacterium-lactis
- molecular analysis
- escherichia-coli
- dominant group
- bacteria
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Dive into the research topics of 'Substrate-driven gene expression in Roseburia inulinivorans: Importance of inducible enzymes in the utilization of inulin and starch'. Together they form a unique fingerprint.Datasets
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GSE22245: Differential gene expression in Roseburia inulinivorans grown on inulin and starch.
Campbell, G. P. (Creator), Scott, K. (Creator), Martin, J. C. (Creator), Chassard, C. (Creator) & Clerget, M. (Creator), GEO, 22 Mar 2012
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22245 and 5 more links, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GPL10020, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM553706, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM553707, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM553708, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM553709 (show fewer)
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