The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C–F bond. We now report that the enzyme will accept 2′-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C–F bond formation.
Cobb, S. L., Deng, H., McEwan, A. R., Naismith, J. H., O'hagan, D., & Robinson, D. A. (2006). Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates. Organic & Biomolecular Chemistry, (8), 1458-1460. https://doi.org/10.1039/B600574H