Abstract
The aoudad (Ammotragus lervia) is a wild ruminant considered the living ancestor of domestic sheep and goats. The original distribution of aoudads includes several countries in the North of Africa, but it has been introduced, for trophy-hunting purposes, into other countries (USA, Mexico and Spain). The species was declared vulnerable in the 2012 IUCN's Red List and is also included in the CITES II Appendix. Surprisingly, little is known about its conservation status or the reproductive biology of the natural populations. There are some reports of the application of basic assisted-reproduction techniques in captive aoudads. In this preliminary study, we explored the feasibility of implementing assisted reproduction procedures in captive aoudad females using non-traditional techniques for in vivo embryo production. This approach was used to obtain the best results using the minimum possible number of animals. Three aoudad females were synchronized using a domestic sheep protocol and subcutaneous osmotic pumps for the delivery of follicle-stimulating hormone. A mini-surgical approach combined with laparoscopy was performed to obtain in vivo-produced embryos. All females had an ovulatory response of more than three corporea lutea, but only five good quality morulae were obtained from one female. Those were cryopreserved by vitrification using a Cryotop®. In conclusion, our approach was successful in obtaining in vivo embryos using a limited number of females. Further studies are necessary to optimize the fertilization rate and clarify the effect of our protocol on embryo implantation and the production of offspring.
Original language | English |
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Pages (from-to) | 149-157 |
Number of pages | 9 |
Journal | Tropical Conservation Science |
Volume | 6 |
Issue number | 1 |
Early online date | 1 Mar 2013 |
DOIs | |
Publication status | Published - Mar 2013 |
Bibliographical note
We thank all of the administrative, veterinary and animal keeper staff of the Leon Zoological Park and the embryologists (especially to Claudia Gonzalez) of the Instituto de Ciencias en Reproducción Humana, also to J.I Castillo-Quan for critical reading of the manuscript. This work was partially supported by grants 0105961/10110/194/09 to RF, 59716 to JPRU from CONACYT and grant 107996 to JPRU from FOMIXYucatán 2008. JLS was supported by a doctoral scholarship from CONACYT (240847).Keywords
- Aoudad
- Cryotop®
- Osmotic pumps
- Superovulation
- Vitrification
- Wild ruminant