Abstract
Scope
The 9cis,11trans-conjugated linoleic acid (9c,11t-CLA) is reported to have anti-atherogenic properties in animal models and to modulate protein expression in unstimulated human platelets in vivo. Platelet function was therefore investigated after dietary supplementation with 9c,11t-CLA enriched oil (CLA80:20) in a randomized, baseline-controlled cross-over trial.
Methods and results
Forty-three healthy adults at low to moderate risk of cardiovascular disease received 4 g/day of CLA80:20 or placebo for two weeks each. Platelet function, inflammation, and endothelial activation were assessed before and after each phase. Compared with placebo, supplementation had no significant effects on platelet function measured by Platelet Function Analyzer-100. Inhibitory effects on collagen-induced aggregation were sex-dependent (p = 0.005) that reached significance only in women (p = 0.045). Thrombin receptor-activating peptide (TRAP)-induced P-selectin expression was higher after supplementation in all subjects (p = 0.017). TRAP-induced platelet fibrinogen binding was also dependent on sex (p = 0.015), with fibrinogen binding after CLA80:20 being higher in males (p = 0.035). Plasma monocyte chemoattractant protein-1 was higher (p = 0.041) after CLA80:20.
Conclusion
No clear evidence was found for inhibition or activation of platelet function as well as inflammation by CLA80:20 in a low to moderate cardiovascular risk group.
The 9cis,11trans-conjugated linoleic acid (9c,11t-CLA) is reported to have anti-atherogenic properties in animal models and to modulate protein expression in unstimulated human platelets in vivo. Platelet function was therefore investigated after dietary supplementation with 9c,11t-CLA enriched oil (CLA80:20) in a randomized, baseline-controlled cross-over trial.
Methods and results
Forty-three healthy adults at low to moderate risk of cardiovascular disease received 4 g/day of CLA80:20 or placebo for two weeks each. Platelet function, inflammation, and endothelial activation were assessed before and after each phase. Compared with placebo, supplementation had no significant effects on platelet function measured by Platelet Function Analyzer-100. Inhibitory effects on collagen-induced aggregation were sex-dependent (p = 0.005) that reached significance only in women (p = 0.045). Thrombin receptor-activating peptide (TRAP)-induced P-selectin expression was higher after supplementation in all subjects (p = 0.017). TRAP-induced platelet fibrinogen binding was also dependent on sex (p = 0.015), with fibrinogen binding after CLA80:20 being higher in males (p = 0.035). Plasma monocyte chemoattractant protein-1 was higher (p = 0.041) after CLA80:20.
Conclusion
No clear evidence was found for inhibition or activation of platelet function as well as inflammation by CLA80:20 in a low to moderate cardiovascular risk group.
| Original language | English |
|---|---|
| Pages (from-to) | 741-750 |
| Number of pages | 10 |
| Journal | Molecular Nutrition & Food Research |
| Volume | 59 |
| Issue number | 4 |
| Early online date | 23 Feb 2015 |
| DOIs | |
| Publication status | Published - Apr 2015 |
Bibliographical note
Isobel Ford and Baukje de Roos are joint senior authors on this publication.Acknowledgement
The authors thank all the volunteers for participating in this study. The personnel of the Human Nutrition Unit at the Rowett Institute of Nutrition and Health provided excellent and expert contributions to the conduct of the trial.
The Rowett Institute of Nutrition and Health and Biomathematics and Statistics Scotland receive funding from the Scottish Government Rural and Environment Science and Analytical Services (RESAS). E.M.B. received a scholarship partly funded by Stepan Specialty Products BV, The Netherlands. The supplement was provided and the trial funded by Stepan Specialty Products BV, The Netherlands.
Potential conflict of interest statement: Dr. Eva-Maria Bachmair received a scholarship partly funded by Stepan Specialty Products BV during the trial. Hiskias G. Keizer is employee of Stepan Specialty Products BV. Mrs. Sharon G. Wood, Dr. Graham W. Horgan, Dr. Isobel Ford, and Dr. Baukje de Roos report no disclosures. The sponsor (represented by H.G.K.) was given the drafts for comments and suggestions but E.M.B., I.F., and B.d.R. had primary responsibility for data analysis and final content
Keywords
- cardiovascular risk
- platelet function
- dietary intervention
- conjugated linoleic acid
- fatty-acids
- randomized controlled trial
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Baukje de Roos
- School of Medicine, Medical Sciences & Nutrition, The Rowett Institute of Nutrition and Health - Personal Chair
Person: Academic