Surface-enhanced Raman signatures of pigmentation of cyanobacteria from within geological samples in a spectroscopic-microfluidic flow cell

Rab Wilson, Paul Monaghan, Stephen A. Bowden, John Parnell, Jonathan M. Cooper

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A simple surface-enhanced Raman spectroscopy (SERS) microflow cell was developed to investigate distributions of scytonemin pigment within cyanobacteria, from samples of rock collected from an arctic desert that contained endolithic cyanobacteria. The assay, which has future potential use in a variety of applications, including astrobiology and analysis of microorganisms in remote environments, involved studying SERS spectra of bacteria from within geological samples. By using a dispersed colloidal substrate in the microfluidic device, surface enhancement of the order > 10(5) was obtained for the determination of the pigment in the microorganisms when compared to the native Raman spectra. The SERS assay, which had a nM sensitivity for scytonemin, showed that the concentration of pigment was highest in samples that had experienced the highest stress environments, as a result of high doses of UV irradiation.

Original languageEnglish
Pages (from-to)7036-7041
Number of pages6
JournalAnalytical Chemistry
Volume79
Issue number18
Early online date21 Aug 2007
DOIs
Publication statusPublished - 15 Sep 2007

Keywords

  • natural-populations
  • calothrix SP.
  • scytomenin
  • scattering
  • spectra
  • carotenoids
  • performance
  • community
  • habitats
  • systems

Cite this

Surface-enhanced Raman signatures of pigmentation of cyanobacteria from within geological samples in a spectroscopic-microfluidic flow cell. / Wilson, Rab; Monaghan, Paul; Bowden, Stephen A.; Parnell, John; Cooper, Jonathan M.

In: Analytical Chemistry, Vol. 79, No. 18, 15.09.2007, p. 7036-7041.

Research output: Contribution to journalArticle

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KW - habitats

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