Survey of the camel urinary proteome by shotgun proteomics using a multiple database search strategy

Abdulqader A. Alhaider, Nervana Bayoumi, Evelyn Argo, Abdel G. M. A. Gader, David A. Stead

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report the first survey of the dromedary camel urinary proteome. Proteins retained from ultrafiltration of urine were analysed by GeLC-MS/MS (SDS-PAGE followed by LC-MS/MS). In the absence of a complete camel genome sequence, the number of protein identifications was maximised by searching three primary sequence databases: Swiss-Prot, alpaca and camel EST. This search strategy enabled the identification of 1274 peptide sequences, of which 735 were found in at least two independent samples. Functional annotations for proteins identified from alpaca and camel EST sequences were mapped from basic local alignment search tool (protein) searches. These 735 peptides, which included many novel sequences found only in the camel EST database, were grouped to 147 protein descriptors. Gene ontology term analysis of human proteins with sequence similarity showed that camel urine may be particularly enriched in proteins from extracellular compartments and vesicles, and with functions that include carbohydrate-binding and peptidase inhibitor activities. If their biological functions are conserved between species, many of the camel urinary proteins could be involved in various stress and immune responses, and some may have antimicrobial activities.
Original languageEnglish
Pages (from-to)3403-3406
Number of pages4
JournalProteomics
Volume12
Issue number22
Early online date19 Oct 2012
DOIs
Publication statusPublished - Nov 2012

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Camelus
Firearms
Proteome
Proteomics
Databases
Expressed Sequence Tags
Proteins
New World Camelids
Genes
Urine
Molecular Sequence Annotation
Peptides
Gene Ontology
Surveys and Questionnaires
Ultrafiltration
Protease Inhibitors
Ontology
Polyacrylamide Gel Electrophoresis
Carbohydrates
Genome

Cite this

Survey of the camel urinary proteome by shotgun proteomics using a multiple database search strategy. / Alhaider, Abdulqader A.; Bayoumi, Nervana; Argo, Evelyn; Gader, Abdel G. M. A.; Stead, David A.

In: Proteomics, Vol. 12, No. 22, 11.2012, p. 3403-3406.

Research output: Contribution to journalArticle

Alhaider, Abdulqader A. ; Bayoumi, Nervana ; Argo, Evelyn ; Gader, Abdel G. M. A. ; Stead, David A. / Survey of the camel urinary proteome by shotgun proteomics using a multiple database search strategy. In: Proteomics. 2012 ; Vol. 12, No. 22. pp. 3403-3406.
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