SURVIVAL AND ACTIVITY OF LUX-MARKED AEROMONAS-SALMONICIDA IN SEAWATER

Yvonne Moira Ferguson, Lesley Anne Glover, D M MCGILLIVRAY, James Ivor Prosser

Research output: Contribution to journalComment/debate

25 Citations (Scopus)

Abstract

The fish pathogen Aeromonas salmonicida was chromosomally marked with genes encoding bacterial luciferase, luxAB, isolated from Vibro fischeri, resulting in constitutive luciferase production. During exponential growth in liquid batch culture, luminescence was directly proportional to biomass concentration, and luminometry provided a lower detection limit of approximately 10(3) cells ml(-1), 1 order of magnitude more sensitive than enzyme-linked immunosorbent assay detection. In sterile seawater at 4 degrees C, lax-marked A. salmonicida entered a dormant, nonculturable state and population activity decreased rapidly. The activity per viable cell, however, increased by day 4, indicating that a proportion of the population remained active and culturable. Putative dormant cells were not resuscitated after the addition of a range of substrates.

Original languageEnglish
Pages (from-to)3494-3498
Number of pages5
JournalApplied and Environmental Microbiology
Volume61
Issue number9
Publication statusPublished - Sep 1995

Keywords

  • GENETICALLY ENGINEERED MICROORGANISMS
  • PSEUDOMONAS-FLUORESCENS
  • CHROMOSOMAL INSERTION
  • SUBSP SALMONICIDA
  • LAKE WATER
  • SOIL
  • BIOLUMINESCENCE
  • ENVIRONMENT
  • BACTERIA
  • SYSTEMS

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