Synaptic Ras GTPase activating protein regulates pattern formation in the trigeminal system of mice

Mark W Barnett, Ruth F Watson, Tania Vitalis, Karen Porter, Noboru H Komiyama, Patrick Niall Stoney, Thomas H Gillingwater, Seth G N Grant, Peter C Kind

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The development of ordered connections or "maps" within the nervous system is a common feature of sensory systems and is crucial for their normal function. NMDA receptors are known to play a key role in the formation of these maps; however, the intracellular signaling pathways that mediate the effects of glutamate are poorly understood. Here, we demonstrate that SynGAP, a synaptic Ras GTPase activating protein, is essential for the anatomical development of whisker-related patterns in the developing somatosensory pathways in rodent forebrain. Mice lacking SynGAP show only partial segregation of barreloids in the thalamus, and thalamocortical axons segregate into rows but do not form whisker-related patches. In cortex, layer 4 cells do not aggregate to form barrels. In Syngap(+/-) animals, barreloids develop normally, and thalamocortical afferents segregate in layer 4, but cell segregation is retarded. SynGAP is not necessary for the development of whisker-related patterns in the brainstem. Immunoelectron microscopy for SynGAP from layer 4 revealed a postsynaptic localization with labeling in developing postsynaptic densities (PSDs). Biochemically, SynGAP associates with the PSD in a PSD-95-independent manner, and Psd-95(-/-) animals develop normal barrels. These data demonstrate an essential role for SynGAP signaling in the activity-dependent development of whisker-related maps selectively in forebrain structures indicating that the intracellular pathways by which NMDA receptor activation mediates map formation differ between brain regions and developmental stage.

Original languageEnglish
Pages (from-to)1355-1365
Number of pages11
JournalJournal of Neuroscience
Volume26
Issue number5
DOIs
Publication statusPublished - 1 Feb 2006

Fingerprint

ras GTPase-Activating Proteins
Vibrissae
Post-Synaptic Density
Prosencephalon
N-Methyl-D-Aspartate Receptors
Immunoelectron Microscopy
Cell Separation
Thalamus
Nervous System
Brain Stem
Axons
Glutamic Acid
Rodentia
Brain

Keywords

  • Animals
  • Body Patterning
  • Guanylate Kinase
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Mice
  • Mice, Knockout
  • Proto-Oncogene Proteins p21(ras)
  • Somatosensory Cortex
  • Thalamus
  • Trigeminal Nuclei
  • ras GTPase-Activating Proteins

Cite this

Barnett, M. W., Watson, R. F., Vitalis, T., Porter, K., Komiyama, N. H., Stoney, P. N., ... Kind, P. C. (2006). Synaptic Ras GTPase activating protein regulates pattern formation in the trigeminal system of mice. Journal of Neuroscience, 26(5), 1355-1365. https://doi.org/10.1523/JNEUROSCI.3164-05.2006

Synaptic Ras GTPase activating protein regulates pattern formation in the trigeminal system of mice. / Barnett, Mark W; Watson, Ruth F; Vitalis, Tania; Porter, Karen; Komiyama, Noboru H; Stoney, Patrick Niall; Gillingwater, Thomas H; Grant, Seth G N; Kind, Peter C.

In: Journal of Neuroscience, Vol. 26, No. 5, 01.02.2006, p. 1355-1365.

Research output: Contribution to journalArticle

Barnett, MW, Watson, RF, Vitalis, T, Porter, K, Komiyama, NH, Stoney, PN, Gillingwater, TH, Grant, SGN & Kind, PC 2006, 'Synaptic Ras GTPase activating protein regulates pattern formation in the trigeminal system of mice', Journal of Neuroscience, vol. 26, no. 5, pp. 1355-1365. https://doi.org/10.1523/JNEUROSCI.3164-05.2006
Barnett, Mark W ; Watson, Ruth F ; Vitalis, Tania ; Porter, Karen ; Komiyama, Noboru H ; Stoney, Patrick Niall ; Gillingwater, Thomas H ; Grant, Seth G N ; Kind, Peter C. / Synaptic Ras GTPase activating protein regulates pattern formation in the trigeminal system of mice. In: Journal of Neuroscience. 2006 ; Vol. 26, No. 5. pp. 1355-1365.
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