Synchronization modulation increases transepithelial potentials in MDCK monolayers through Na/K pumps

Vu Tran, Xiaodong Zhang, Lin Cao, Hanqing Li, Benjamin Lee, Michelle So, Yaohui Sun, Wei Chen, Min Zhao

Research output: Contribution to journalArticle

3 Citations (Scopus)
4 Downloads (Pure)

Abstract

Transepithelial potential (TEP) is the voltage across a polarized epithelium. In epithelia that have active transport functions, the force for transmembrane flux of an ion is dictated by the electrochemical gradient in which TEP plays an essential role. In epithelial injury, disruption of the epithelial barrier collapses the TEP at the wound edge, resulting in the establishment of an endogenous wound electric field (∼100 mV/mm) that is directed towards the center of the wound. This endogenous electric field is implicated to enhance wound healing by guiding cell migration. We thus seek techniques to enhance the TEP, which may increase the wound electric fields and enhance wound healing. We report a novel technique, termed synchronization modulation (SM) using a train of electric pulses to synchronize the Na/K pump activity, and then modulating the pumping cycles to increase the efficiency of the Na/K pumps. Kidney epithelial monolayers (MDCK cells) maintain a stable TEP and transepithelial resistance (TER). SM significantly increased TEP over four fold. Either ouabain or digoxin, which block Na/K pump, abolished SM-induced TEP increases. In addition to the pump activity, basolateral distribution of Na/K pumps is essential for an increase in TEP. Our study for the first time developed an electrical approach to significantly increase the TEP. This technique targeting the Na/K pump may be used to modulate TEP, and may have implication in wound healing and in diseases where TEP needs to be modulated.

Original languageEnglish
Article numbere61509
JournalPloS ONE
Volume8
Issue number4
DOIs
Publication statusPublished - 9 Apr 2013

Fingerprint

sodium-potassium-exchanging ATPase
Monolayers
Synchronization
animal injuries
Modulation
Pumps
electric field
tissue repair
Wound Healing
Wounds and Injuries
Electric fields
epithelium
Epithelium
digoxin
ouabain
Madin Darby Canine Kidney Cells
active transport
Active Biological Transport
Digoxin
Ouabain

Keywords

  • Animals
  • Cell Movement
  • Digoxin
  • Dogs
  • Electric Stimulation
  • Enzyme Inhibitors
  • Ion Transport
  • Madin Darby Canine Kidney Cells
  • Membrane Potentials
  • Models, Biological
  • Ouabain
  • Protein Transport
  • Sodium-Potassium-Exchanging ATPase
  • Wound Healing
  • Wounds and Injuries

Cite this

Synchronization modulation increases transepithelial potentials in MDCK monolayers through Na/K pumps. / Tran, Vu; Zhang, Xiaodong; Cao, Lin; Li, Hanqing; Lee, Benjamin; So, Michelle; Sun, Yaohui; Chen, Wei; Zhao, Min.

In: PloS ONE, Vol. 8, No. 4, e61509, 09.04.2013.

Research output: Contribution to journalArticle

Tran, Vu ; Zhang, Xiaodong ; Cao, Lin ; Li, Hanqing ; Lee, Benjamin ; So, Michelle ; Sun, Yaohui ; Chen, Wei ; Zhao, Min. / Synchronization modulation increases transepithelial potentials in MDCK monolayers through Na/K pumps. In: PloS ONE. 2013 ; Vol. 8, No. 4.
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AU - Tran, Vu

AU - Zhang, Xiaodong

AU - Cao, Lin

AU - Li, Hanqing

AU - Lee, Benjamin

AU - So, Michelle

AU - Sun, Yaohui

AU - Chen, Wei

AU - Zhao, Min

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AB - Transepithelial potential (TEP) is the voltage across a polarized epithelium. In epithelia that have active transport functions, the force for transmembrane flux of an ion is dictated by the electrochemical gradient in which TEP plays an essential role. In epithelial injury, disruption of the epithelial barrier collapses the TEP at the wound edge, resulting in the establishment of an endogenous wound electric field (∼100 mV/mm) that is directed towards the center of the wound. This endogenous electric field is implicated to enhance wound healing by guiding cell migration. We thus seek techniques to enhance the TEP, which may increase the wound electric fields and enhance wound healing. We report a novel technique, termed synchronization modulation (SM) using a train of electric pulses to synchronize the Na/K pump activity, and then modulating the pumping cycles to increase the efficiency of the Na/K pumps. Kidney epithelial monolayers (MDCK cells) maintain a stable TEP and transepithelial resistance (TER). SM significantly increased TEP over four fold. Either ouabain or digoxin, which block Na/K pump, abolished SM-induced TEP increases. In addition to the pump activity, basolateral distribution of Na/K pumps is essential for an increase in TEP. Our study for the first time developed an electrical approach to significantly increase the TEP. This technique targeting the Na/K pump may be used to modulate TEP, and may have implication in wound healing and in diseases where TEP needs to be modulated.

KW - Animals

KW - Cell Movement

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KW - Dogs

KW - Electric Stimulation

KW - Enzyme Inhibitors

KW - Ion Transport

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KW - Membrane Potentials

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KW - Ouabain

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