Systemic conditioned medium treatment from interleukin-1 primed mesenchymal stem cells promotes recovery after stroke

Catriona J Cunningham, Raymond Wong, Jack Barrington, Sabrina Tamburrano, Emmanuel Pinteaux, Stuart M. Allan*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)
3 Downloads (Pure)

Abstract

Background
Mesenchymal stem cells (MSCs) hold great potential as a therapy for stroke and have previously been shown to promote recovery in preclinical models of cerebral ischaemia. MSCs secrete a wide range of growth factors, chemokines, cytokines and extracellular vesicles—collectively termed the secretome. In this study, we assessed for the first time the efficacy of the IL-1α-primed MSC-derived secretome on brain injury and functional recovery after cerebral ischaemia.

Methods
Stroke was induced in male C57BL/6 mice using the intraluminal filament model of middle cerebral artery occlusion. Conditioned medium from IL-1α-primed MSCs or vehicle was administered at the time of reperfusion or at 24 h post-stroke by subcutaneous injection.

Results
IL-1α-primed MSC-derived conditioned medium treatment at the time of stroke led to a ~ 30% reduction in lesion volume at 48 h and was associated with modest improvements in body mass gain, 28-point neurological score and nest building. Administration of MSC-derived conditioned medium at 24 h post-stroke led to improved nest building and neurological score despite no observed differences in lesion volume at day 2 post-stroke.

Conclusions
Our results show for the first time that the administration of conditioned medium from IL-1α-primed MSCs leads to improvements in behavioural outcomes independently of neuroprotection.
Original languageEnglish
Article number32
JournalStem cell research & therapy
Volume11
DOIs
Publication statusPublished - 21 Jan 2020

Keywords

  • cell therapy
  • conditioned medium
  • mesenchymal stem cell
  • secretome
  • stroke
  • Mesenchymal stem cell
  • Stroke
  • Cell therapy
  • Conditioned medium
  • Secretome
  • FUNCTIONAL RECOVERY
  • BEHAVIOR
  • MODEL
  • NEUROPROTECTION
  • TRANSPLANTATION
  • INFLAMMATION
  • THERAPEUTIC BENEFIT
  • CEREBRAL-ISCHEMIA
  • BRAIN
  • MARROW STROMAL CELLS

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