T-cell specificity in murine autoimmune haemolytic anaemia induced by rat red blood cells

Robert Norman Barker, C. R. Shen, C. J. Elson

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15 Citations (Scopus)

Abstract

Autoimmune haemolytic anaemia (AIHA) can be induced in mice by repeated injections with rat red blood cells (RBC). Here we describe the identification of rat and murine RBC antigens recognized by T-cells from mice with this disease. Splenic T-cells from mice with AIHA proliferated in response to multiple murine RBC membrane components, each of which is recognized by rat RBC induced autoantibodies. Thus, there were responses to murine autoantigen fractions that correspond in apparent molecular mass with the anion channel Band 3, with spectrin from the membrane skeleton and with the high and low molecular mass glycophorins, and the equivalent fractions from rat RBC also stimulated proliferation by T-cells. It was confirmed that purified Band 3 from murine and rat RBC also elicited responses. In contrast with the results in AIHA, T-cells from healthy control mice failed to respond to the antigens from either species, with the exception of proliferation induced by murine spectrin in one experiment and weak responses elicited by rat Band 3. It is suggested that T-cells activated by multiple cross-reactions between rat and murine RBC proteins, and by epitope spreading, are necessary to drive autoantibody production in this model of AIHA.

Original languageEnglish
Pages (from-to)208-213
Number of pages5
JournalClinical and Experimental Immunology
Volume129
Issue number2
DOIs
Publication statusPublished - 2002

Keywords

  • T-cell autoantigens
  • erythrocyte band 3
  • autoimmune haemolytic anaemia
  • HEMOLYTIC-ANEMIA
  • ERYTHROCYTE AUTOANTIBODIES
  • BAND-3 PROTEIN
  • NORMAL MICE
  • B-CELL
  • IDENTIFICATION
  • AUTOANTIGENS
  • ANTIGENS
  • MEMBRANE
  • EPITOPES

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