@inbook{72b5080181434a0d800fb0ccd9a43008,
title = "Tandem Mass Tags for Comparative and Discovery Proteomics",
abstract = "Relative or comparative proteomics provides valuable insights about the altered protein abundances across different biological samples in a single (labeled) or series (label-free) of LC-MS measurement(s). Chemical labeling of peptides using isobaric mass tags for identification and quantification of different proteomes simultaneously has become a routine in the so-called discovery proteomics in the past decade. One of the earliest isobaric tags-based technologies is TMT (tandem mass tags), which relies on the comparison of the unique {"}reporter ions{"} intensities for relative peptide/protein quantification. This differential labeling approach has evolved over time with respect to its multiplexing capability, i.e., from just 2 samples (TMTduplex) to 10 samples (TMT10plex) and a nowadays of up to 16 samples (TMTpro 16plex). Here, we describe a straightforward protocol to perform relatively deep proteome quantitative analyses using TMT10plex.",
keywords = "LC–MS/MS, Multiplexing, Relative quantitative proteomics, TMT",
author = "Oliver Pagel and Laxmikanth Kollipara and Albert Sickmann",
note = "Acknowledgments: OP, LK, and AS acknowledge the support by the Ministerium f{\"u}r Kultur und Wissenschaft des Landes Nordrhein-Westfalen, the Regierende B{\"u}rgermeister von Berlin—inkl. Wissenschaft und Forschung, and the Bundesministerium f{\"u}r Bildung und Forschung.",
year = "2021",
doi = "10.1007/978-1-0716-1024-4_9",
language = "English",
isbn = "978-1-0716-1023-7",
volume = "2228",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "117--131",
editor = "Katrin Marcus and Eisenacher, {Martin } and Barbara Sitek",
booktitle = "Quantitative Methods in Proteomics",
address = "United States",
edition = "2nd",
}