Targeting of calcium: Calmodulin signals to the cytoskeleton by IQGAP1

Maria Psatha, Minoo Razi, Anna Koffer, Steve E. Moss, David B. Sacks, Stephen R. Bolsover

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Mast cells reorganize their actin cytoskeleton in response to cytosolic calcium signals while in parallel secreting histamine and other inflammatory mediators. The effect of calcium on actin is mediated in large part through calmodulin. EGFP-tagged calmodulin is concentrated in the actin-rich cortex of RBL-2H3 mast cells. Transfection with small interfering RNA directed against the actin and calmodulin-binding protein IQGAP1 dramatically reduced expression of the latter protein and reduced or eliminated the concentration of calmodulin at the actin-rich cortex. Both actin reorganization and secretion were enhanced in IQGAP1 knockdown cells. Our results suggest a model in which calmodulin is targeted to and sequestered at the actin cytoskeleton by IQGAP1. Upon cell stimulation and the subsequent [Ca2+]i increase, it is immediately available to activate local downstream targets.
Original languageEnglish
Pages (from-to)593-605
Number of pages13
JournalCell Calcium
Volume41
Issue number6
Early online date29 Nov 2006
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Calmodulin
Cytoskeleton
Actins
Calcium
Actin Cytoskeleton
Mast Cells
Calmodulin-Binding Proteins
Microfilament Proteins
Histamine
Small Interfering RNA
Transfection
Proteins

Keywords

  • animals
  • calcium
  • calcium signaling
  • calmodulin
  • cell line, tumor
  • cytoskeleton
  • immunoblotting
  • mast cells
  • microscopy, confocal
  • microscopy, fluorescence
  • rats
  • ras GTPase-activating proteins

Cite this

Psatha, M., Razi, M., Koffer, A., Moss, S. E., Sacks, D. B., & Bolsover, S. R. (2007). Targeting of calcium: Calmodulin signals to the cytoskeleton by IQGAP1. Cell Calcium, 41(6), 593-605. https://doi.org/10.1016/j.ceca.2006.10.009

Targeting of calcium : Calmodulin signals to the cytoskeleton by IQGAP1. / Psatha, Maria; Razi, Minoo; Koffer, Anna; Moss, Steve E.; Sacks, David B.; Bolsover, Stephen R.

In: Cell Calcium, Vol. 41, No. 6, 06.2007, p. 593-605.

Research output: Contribution to journalArticle

Psatha, M, Razi, M, Koffer, A, Moss, SE, Sacks, DB & Bolsover, SR 2007, 'Targeting of calcium: Calmodulin signals to the cytoskeleton by IQGAP1', Cell Calcium, vol. 41, no. 6, pp. 593-605. https://doi.org/10.1016/j.ceca.2006.10.009
Psatha M, Razi M, Koffer A, Moss SE, Sacks DB, Bolsover SR. Targeting of calcium: Calmodulin signals to the cytoskeleton by IQGAP1. Cell Calcium. 2007 Jun;41(6):593-605. https://doi.org/10.1016/j.ceca.2006.10.009
Psatha, Maria ; Razi, Minoo ; Koffer, Anna ; Moss, Steve E. ; Sacks, David B. ; Bolsover, Stephen R. / Targeting of calcium : Calmodulin signals to the cytoskeleton by IQGAP1. In: Cell Calcium. 2007 ; Vol. 41, No. 6. pp. 593-605.
@article{1a604f78758f4783a735f76acd5d785b,
title = "Targeting of calcium: Calmodulin signals to the cytoskeleton by IQGAP1",
abstract = "Mast cells reorganize their actin cytoskeleton in response to cytosolic calcium signals while in parallel secreting histamine and other inflammatory mediators. The effect of calcium on actin is mediated in large part through calmodulin. EGFP-tagged calmodulin is concentrated in the actin-rich cortex of RBL-2H3 mast cells. Transfection with small interfering RNA directed against the actin and calmodulin-binding protein IQGAP1 dramatically reduced expression of the latter protein and reduced or eliminated the concentration of calmodulin at the actin-rich cortex. Both actin reorganization and secretion were enhanced in IQGAP1 knockdown cells. Our results suggest a model in which calmodulin is targeted to and sequestered at the actin cytoskeleton by IQGAP1. Upon cell stimulation and the subsequent [Ca2+]i increase, it is immediately available to activate local downstream targets.",
keywords = "animals, calcium, calcium signaling, calmodulin, cell line, tumor, cytoskeleton, immunoblotting, mast cells, microscopy, confocal, microscopy, fluorescence, rats, ras GTPase-activating proteins",
author = "Maria Psatha and Minoo Razi and Anna Koffer and Moss, {Steve E.} and Sacks, {David B.} and Bolsover, {Stephen R.}",
year = "2007",
month = "6",
doi = "10.1016/j.ceca.2006.10.009",
language = "English",
volume = "41",
pages = "593--605",
journal = "Cell Calcium",
issn = "0143-4160",
publisher = "Churchill Livingstone",
number = "6",

}

TY - JOUR

T1 - Targeting of calcium

T2 - Calmodulin signals to the cytoskeleton by IQGAP1

AU - Psatha, Maria

AU - Razi, Minoo

AU - Koffer, Anna

AU - Moss, Steve E.

AU - Sacks, David B.

AU - Bolsover, Stephen R.

PY - 2007/6

Y1 - 2007/6

N2 - Mast cells reorganize their actin cytoskeleton in response to cytosolic calcium signals while in parallel secreting histamine and other inflammatory mediators. The effect of calcium on actin is mediated in large part through calmodulin. EGFP-tagged calmodulin is concentrated in the actin-rich cortex of RBL-2H3 mast cells. Transfection with small interfering RNA directed against the actin and calmodulin-binding protein IQGAP1 dramatically reduced expression of the latter protein and reduced or eliminated the concentration of calmodulin at the actin-rich cortex. Both actin reorganization and secretion were enhanced in IQGAP1 knockdown cells. Our results suggest a model in which calmodulin is targeted to and sequestered at the actin cytoskeleton by IQGAP1. Upon cell stimulation and the subsequent [Ca2+]i increase, it is immediately available to activate local downstream targets.

AB - Mast cells reorganize their actin cytoskeleton in response to cytosolic calcium signals while in parallel secreting histamine and other inflammatory mediators. The effect of calcium on actin is mediated in large part through calmodulin. EGFP-tagged calmodulin is concentrated in the actin-rich cortex of RBL-2H3 mast cells. Transfection with small interfering RNA directed against the actin and calmodulin-binding protein IQGAP1 dramatically reduced expression of the latter protein and reduced or eliminated the concentration of calmodulin at the actin-rich cortex. Both actin reorganization and secretion were enhanced in IQGAP1 knockdown cells. Our results suggest a model in which calmodulin is targeted to and sequestered at the actin cytoskeleton by IQGAP1. Upon cell stimulation and the subsequent [Ca2+]i increase, it is immediately available to activate local downstream targets.

KW - animals

KW - calcium

KW - calcium signaling

KW - calmodulin

KW - cell line, tumor

KW - cytoskeleton

KW - immunoblotting

KW - mast cells

KW - microscopy, confocal

KW - microscopy, fluorescence

KW - rats

KW - ras GTPase-activating proteins

U2 - 10.1016/j.ceca.2006.10.009

DO - 10.1016/j.ceca.2006.10.009

M3 - Article

C2 - 17137623

VL - 41

SP - 593

EP - 605

JO - Cell Calcium

JF - Cell Calcium

SN - 0143-4160

IS - 6

ER -