Targeting the D Series Resolvin Receptor System for the Treatment of Osteoarthritis Pain

Junting Huang, James J Burston (Corresponding Author), Li Li, Sadaf Ashraf, Paul I Mapp, Andrew J Bennett, Srinivasarao Ravipati, Petros Pousinis, David A Barrett, Brigitte E Scammell, Victoria Chapman

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE: Pain is a major symptom of osteoarthritis (OA); currently available analgesics either do not provide adequate pain relief or are associated with serious side effects. The aim of this study was to investigate the therapeutic potential of targeting the resolvin receptor system to modify OA pain and pathology.

METHODS: Gene expression of 2 resolvin receptors (ALX and ChemR23) was quantified in synovium and medial tibial plateau specimens obtained from patients with OA at the time of joint replacement surgery. Two models of OA joint pain were used for the mechanistic studies. Gene expression in the joint and central nervous system was quantified. The effects of exogenous administration of the D series resolvin precursor 17(R)-hydroxy-docosahexaenoic acid (17[R]-HDoHE) on pain behavior, joint pathology, spinal microglia, and astroglyosis were quantified. Plasma levels of relevant lipids, resolvin D2, 17(R)-HDoHE, and arachidonic acid, were determined in rats, using liquid chromatography tandem mass spectrometry.

RESULTS: There was a positive correlation between resolvin receptor and interleukin-6 (IL-6) expression in human OA synovial and medial tibial plateau tissue. In rats, synovial expression of ALX was positively correlated with expression of IL-1β, tumor necrosis factor, and cyclooxygenase 2. Treatment with 17(R)-HDoHE reversed established pain behavior (but not joint pathology) in 2 models of OA pain. This was associated with a significant elevation in the plasma levels of resolvin D2 and a significant reduction in astrogliosis in the spinal cord in the monosodium iodoacetate-induced OA rat model.

CONCLUSION: Our preclinical data demonstrate the robust analgesic effects of activation of the D series resolvin pathways in 2 different animal models of OA. Our data support a predominant central mechanism of action in clinically relevant models of OA pain.

Original languageEnglish
Pages (from-to)996-1008
Number of pages13
JournalArthritis & Rheumatology
Volume69
Issue number5
Early online date16 Nov 2016
DOIs
Publication statusPublished - May 2017

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Osteoarthritis
Pain
Therapeutics
Arthralgia
Pathology
Analgesics
Replacement Arthroplasties
Joints
Iodoacetates
Interleukin-6 Receptors
Gene Expression
Hydroxy Acids
Synovial Membrane
Docosahexaenoic Acids
Microglia
Cyclooxygenase 2
Tandem Mass Spectrometry
Interleukin-1
Arachidonic Acid
Liquid Chromatography

Keywords

  • Adaptor Proteins, Signal Transducing/genetics
  • Animals
  • Arthralgia/chemically induced
  • Behavior, Animal/drug effects
  • Cartilage, Articular/pathology
  • Disease Models, Animal
  • Docosahexaenoic Acids/pharmacology
  • Enzyme Inhibitors/toxicity
  • Gene Expression
  • Humans
  • Iodoacetic Acid/toxicity
  • Menisci, Tibial/surgery
  • Neuroglia/cytology
  • Osteoarthritis, Knee/genetics
  • RNA, Messenger/metabolism
  • Rats
  • Real-Time Polymerase Chain Reaction
  • Receptors, Chemokine/drug effects
  • Receptors, Lipoxin/drug effects
  • Spinal Cord/cytology
  • Synovial Membrane/pathology

Cite this

Huang, J., Burston, J. J., Li, L., Ashraf, S., Mapp, P. I., Bennett, A. J., ... Chapman, V. (2017). Targeting the D Series Resolvin Receptor System for the Treatment of Osteoarthritis Pain. Arthritis & Rheumatology, 69(5), 996-1008. https://doi.org/10.1002/art.40001

Targeting the D Series Resolvin Receptor System for the Treatment of Osteoarthritis Pain. / Huang, Junting; Burston, James J (Corresponding Author); Li, Li; Ashraf, Sadaf; Mapp, Paul I; Bennett, Andrew J; Ravipati, Srinivasarao; Pousinis, Petros; Barrett, David A; Scammell, Brigitte E; Chapman, Victoria.

In: Arthritis & Rheumatology, Vol. 69, No. 5, 05.2017, p. 996-1008.

Research output: Contribution to journalArticle

Huang, J, Burston, JJ, Li, L, Ashraf, S, Mapp, PI, Bennett, AJ, Ravipati, S, Pousinis, P, Barrett, DA, Scammell, BE & Chapman, V 2017, 'Targeting the D Series Resolvin Receptor System for the Treatment of Osteoarthritis Pain', Arthritis & Rheumatology, vol. 69, no. 5, pp. 996-1008. https://doi.org/10.1002/art.40001
Huang, Junting ; Burston, James J ; Li, Li ; Ashraf, Sadaf ; Mapp, Paul I ; Bennett, Andrew J ; Ravipati, Srinivasarao ; Pousinis, Petros ; Barrett, David A ; Scammell, Brigitte E ; Chapman, Victoria. / Targeting the D Series Resolvin Receptor System for the Treatment of Osteoarthritis Pain. In: Arthritis & Rheumatology. 2017 ; Vol. 69, No. 5. pp. 996-1008.
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AU - Ravipati, Srinivasarao

AU - Pousinis, Petros

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N2 - OBJECTIVE: Pain is a major symptom of osteoarthritis (OA); currently available analgesics either do not provide adequate pain relief or are associated with serious side effects. The aim of this study was to investigate the therapeutic potential of targeting the resolvin receptor system to modify OA pain and pathology.METHODS: Gene expression of 2 resolvin receptors (ALX and ChemR23) was quantified in synovium and medial tibial plateau specimens obtained from patients with OA at the time of joint replacement surgery. Two models of OA joint pain were used for the mechanistic studies. Gene expression in the joint and central nervous system was quantified. The effects of exogenous administration of the D series resolvin precursor 17(R)-hydroxy-docosahexaenoic acid (17[R]-HDoHE) on pain behavior, joint pathology, spinal microglia, and astroglyosis were quantified. Plasma levels of relevant lipids, resolvin D2, 17(R)-HDoHE, and arachidonic acid, were determined in rats, using liquid chromatography tandem mass spectrometry.RESULTS: There was a positive correlation between resolvin receptor and interleukin-6 (IL-6) expression in human OA synovial and medial tibial plateau tissue. In rats, synovial expression of ALX was positively correlated with expression of IL-1β, tumor necrosis factor, and cyclooxygenase 2. Treatment with 17(R)-HDoHE reversed established pain behavior (but not joint pathology) in 2 models of OA pain. This was associated with a significant elevation in the plasma levels of resolvin D2 and a significant reduction in astrogliosis in the spinal cord in the monosodium iodoacetate-induced OA rat model.CONCLUSION: Our preclinical data demonstrate the robust analgesic effects of activation of the D series resolvin pathways in 2 different animal models of OA. Our data support a predominant central mechanism of action in clinically relevant models of OA pain.

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