Temperature responses of soil ammonia-oxidising archaea depend on pH

Cécile Gubry-Rangin*, Breda Novotnik, Ines Mandič-Mulec, Graeme W. Nicol, James I. Prosser

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Ammonia oxidising archaea (AOA) are an abundant and ubiquitously distributed group of soil microorganisms and contribute significantly to nitrogen cycling processes. Soil pH has been identified as a major driver of AOA diversification, but other environmental factors may also be important. The aim of this study was to determine whether soil pH also influenced the temperature range of AOA activity in soil. This was assessed by determining rates of ammonification and net nitrification, and AOA abundance and community composition during incubation of soils with pH in the range 3.6–7.5 at 20, 30 or 40 °C for 30 days. Net nitrification was greatest at 20 or 30 °C, with variation in optimal temperature between soils, net nitrification was not detectable at 40 °C, and mineralisation was greatest at 40 °C. AOA community composition differed following incubation at 20° and 30 °C, presumably through selection and growth of populations with different temperature optima, and, at 40 °C, due to cell death. There was no significant relationship between thaumarchaeotal cell abundance and yield estimated using amoA and 16S rRNA genes, possibly due to amplification of 16S rRNA genes of non-ammonia-oxidising Thaumarchaeota. However, amoA gene abundance and yield were greater at 20 °C than 30 °C in the most acidic soils, with the opposite relationship for the most neutral soils. This is consistent with cultivated lower temperature optima for neutrophilic, rather than acidophilic soil AOA. The results indicate that pH-driven diversification may have consequences for other aspects of AOA physiology including temperature optima for growth and activity in the environment.

Original languageEnglish
Pages (from-to)61-68
Number of pages8
JournalSoil Biology and Biochemistry
Volume106
Early online date24 Dec 2016
DOIs
Publication statusPublished - 1 Mar 2017

Fingerprint

Archaea
Ammonia
ammonia
Soil
Temperature
Nitrification
soil
nitrification
temperature
community composition
soil pH
gene
rRNA Genes
incubation
ribosomal RNA
ammonification
genes
soil microorganism
soil microorganisms
acid soils

Keywords

  • Ammonia-oxidising archaea
  • pH
  • Soil
  • Temperature

ASJC Scopus subject areas

  • Microbiology
  • Soil Science

Cite this

Temperature responses of soil ammonia-oxidising archaea depend on pH. / Gubry-Rangin, Cécile; Novotnik, Breda; Mandič-Mulec, Ines; Nicol, Graeme W.; Prosser, James I.

In: Soil Biology and Biochemistry, Vol. 106, 01.03.2017, p. 61-68.

Research output: Contribution to journalArticle

Gubry-Rangin, Cécile ; Novotnik, Breda ; Mandič-Mulec, Ines ; Nicol, Graeme W. ; Prosser, James I. / Temperature responses of soil ammonia-oxidising archaea depend on pH. In: Soil Biology and Biochemistry. 2017 ; Vol. 106. pp. 61-68.
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N1 - Acknowledgments The authors would like to thank Dr Robin Walker for access to the Woodlands Field experimental plots at the SRUC, Craibstone Estate, Aberdeen. This work was financially supported by Natural Environmental Research Council (standard grant NE/F021909/1; fellowship NE/J019151/1) and the AXA Research Fund.

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N2 - Ammonia oxidising archaea (AOA) are an abundant and ubiquitously distributed group of soil microorganisms and contribute significantly to nitrogen cycling processes. Soil pH has been identified as a major driver of AOA diversification, but other environmental factors may also be important. The aim of this study was to determine whether soil pH also influenced the temperature range of AOA activity in soil. This was assessed by determining rates of ammonification and net nitrification, and AOA abundance and community composition during incubation of soils with pH in the range 3.6–7.5 at 20, 30 or 40 °C for 30 days. Net nitrification was greatest at 20 or 30 °C, with variation in optimal temperature between soils, net nitrification was not detectable at 40 °C, and mineralisation was greatest at 40 °C. AOA community composition differed following incubation at 20° and 30 °C, presumably through selection and growth of populations with different temperature optima, and, at 40 °C, due to cell death. There was no significant relationship between thaumarchaeotal cell abundance and yield estimated using amoA and 16S rRNA genes, possibly due to amplification of 16S rRNA genes of non-ammonia-oxidising Thaumarchaeota. However, amoA gene abundance and yield were greater at 20 °C than 30 °C in the most acidic soils, with the opposite relationship for the most neutral soils. This is consistent with cultivated lower temperature optima for neutrophilic, rather than acidophilic soil AOA. The results indicate that pH-driven diversification may have consequences for other aspects of AOA physiology including temperature optima for growth and activity in the environment.

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