Temporal changes in functional diversity of culturable bacteria populations in Sitka spruce stumps

Claire Murray, Stephen Woodward

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Changes in culturable aerobic bacterial communities of Sitka spruce stumps between 1 and 10 years old were investigated by isolating from the top 55 mm of the stumps. High numbers of bacteria were recovered from 15 mm below the surface of 1- and 2-year-old stumps, particularly in the sapwood, after which numbers dropped by an order of magnitude. Proportions of colony-forming units (cfu) capable of degrading cellulose and chitin increased with stump age, whereas siderophore producers decreased. Colony-forming units were grouped on the basis of 13 phenotypic characteristics, including colony form and colour, Gram stain, heat tolerance, siderophore production and production of various enzymes. Four groups of bacteria were identified; the proportions of the three Gram-negative groups varied with stump age. A quadratic relationship between bacterial diversity and stump age was detected using the Shannon-Weaver diversity index. Bacterial diversity was higher 15 mm below the stump surface than in deeper sampling points. The results are discussed in terms of the possible influence of prokaryotic organisms on the development of decay communities in Sitka spruce stumps.

Original languageEnglish
Pages (from-to)217-235
Number of pages19
JournalForest Pathology
Volume37
Issue number4
DOIs
Publication statusAccepted/In press - Aug 2007

Fingerprint

Picea sitchensis
functional diversity
stumps
siderophore
bacterium
bacteria
chitin
diversity index
cellulose
siderophores
tolerance
enzyme
sampling
sapwood
heat tolerance
bacterial communities
deterioration
color
organisms
enzymes

Keywords

  • in-vitro interactions
  • white fir trees
  • heterobasidion-annosum
  • wood moisture
  • fungi
  • decay
  • community
  • identification
  • basidiospores
  • enumeration

Cite this

Temporal changes in functional diversity of culturable bacteria populations in Sitka spruce stumps. / Murray, Claire; Woodward, Stephen.

In: Forest Pathology, Vol. 37, No. 4, 08.2007, p. 217-235.

Research output: Contribution to journalArticle

@article{3bfe6f5b1d8b46bcb2e2c937637a0ea4,
title = "Temporal changes in functional diversity of culturable bacteria populations in Sitka spruce stumps",
abstract = "Changes in culturable aerobic bacterial communities of Sitka spruce stumps between 1 and 10 years old were investigated by isolating from the top 55 mm of the stumps. High numbers of bacteria were recovered from 15 mm below the surface of 1- and 2-year-old stumps, particularly in the sapwood, after which numbers dropped by an order of magnitude. Proportions of colony-forming units (cfu) capable of degrading cellulose and chitin increased with stump age, whereas siderophore producers decreased. Colony-forming units were grouped on the basis of 13 phenotypic characteristics, including colony form and colour, Gram stain, heat tolerance, siderophore production and production of various enzymes. Four groups of bacteria were identified; the proportions of the three Gram-negative groups varied with stump age. A quadratic relationship between bacterial diversity and stump age was detected using the Shannon-Weaver diversity index. Bacterial diversity was higher 15 mm below the stump surface than in deeper sampling points. The results are discussed in terms of the possible influence of prokaryotic organisms on the development of decay communities in Sitka spruce stumps.",
keywords = "in-vitro interactions, white fir trees, heterobasidion-annosum, wood moisture, fungi, decay, community, identification, basidiospores, enumeration",
author = "Claire Murray and Stephen Woodward",
year = "2007",
month = "8",
doi = "10.1111/j.1439-0329.2007.00490.x",
language = "English",
volume = "37",
pages = "217--235",
journal = "Forest Pathology",
issn = "1437-4781",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Temporal changes in functional diversity of culturable bacteria populations in Sitka spruce stumps

AU - Murray, Claire

AU - Woodward, Stephen

PY - 2007/8

Y1 - 2007/8

N2 - Changes in culturable aerobic bacterial communities of Sitka spruce stumps between 1 and 10 years old were investigated by isolating from the top 55 mm of the stumps. High numbers of bacteria were recovered from 15 mm below the surface of 1- and 2-year-old stumps, particularly in the sapwood, after which numbers dropped by an order of magnitude. Proportions of colony-forming units (cfu) capable of degrading cellulose and chitin increased with stump age, whereas siderophore producers decreased. Colony-forming units were grouped on the basis of 13 phenotypic characteristics, including colony form and colour, Gram stain, heat tolerance, siderophore production and production of various enzymes. Four groups of bacteria were identified; the proportions of the three Gram-negative groups varied with stump age. A quadratic relationship between bacterial diversity and stump age was detected using the Shannon-Weaver diversity index. Bacterial diversity was higher 15 mm below the stump surface than in deeper sampling points. The results are discussed in terms of the possible influence of prokaryotic organisms on the development of decay communities in Sitka spruce stumps.

AB - Changes in culturable aerobic bacterial communities of Sitka spruce stumps between 1 and 10 years old were investigated by isolating from the top 55 mm of the stumps. High numbers of bacteria were recovered from 15 mm below the surface of 1- and 2-year-old stumps, particularly in the sapwood, after which numbers dropped by an order of magnitude. Proportions of colony-forming units (cfu) capable of degrading cellulose and chitin increased with stump age, whereas siderophore producers decreased. Colony-forming units were grouped on the basis of 13 phenotypic characteristics, including colony form and colour, Gram stain, heat tolerance, siderophore production and production of various enzymes. Four groups of bacteria were identified; the proportions of the three Gram-negative groups varied with stump age. A quadratic relationship between bacterial diversity and stump age was detected using the Shannon-Weaver diversity index. Bacterial diversity was higher 15 mm below the stump surface than in deeper sampling points. The results are discussed in terms of the possible influence of prokaryotic organisms on the development of decay communities in Sitka spruce stumps.

KW - in-vitro interactions

KW - white fir trees

KW - heterobasidion-annosum

KW - wood moisture

KW - fungi

KW - decay

KW - community

KW - identification

KW - basidiospores

KW - enumeration

U2 - 10.1111/j.1439-0329.2007.00490.x

DO - 10.1111/j.1439-0329.2007.00490.x

M3 - Article

VL - 37

SP - 217

EP - 235

JO - Forest Pathology

JF - Forest Pathology

SN - 1437-4781

IS - 4

ER -