Temporal events in the intravenous challenge model for experimental Candida albicans infections in female mice

Donna Margaret MacCallum, Frank Christopher Odds

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104 Citations (Scopus)

Abstract

We characterized the intravenous (i.v.) challenge model for disseminated Candida albicans infection in female BALB/c and DBA/2 mice. Clearance of fungi from the bloodstream and appearance of fungi in tissues were measured at intervals after challenge with various doses of C. albicans. The wild-type isolate SC5314 and derived strains CAF2-1 and CAI-4 transformed with CIp10 were of equal virulence in the model. Variability in mouse survival times, kidney fungal burdens and cachexia was lowest when challenge inocula were within the range 10(4)-10(5) CFU g(-1) body weight in BALB/c mice, but brain fungal burdens and outcomes in DBA/2 mice were variable for all inocula tested. Critical times in the development of infections in optimally challenged BALB/c mice were at 5-10 h (bloodstream fully cleared of fungi), 24 h (start of exponential fungal growth in kidneys) and 48 h (50% of blood cultures become positive). Differential involvement of right and left kidneys occurred almost exclusively in mice challenged with
Original languageEnglish
Pages (from-to)151-61
Number of pages11
JournalMycoses
Volume48
Issue number3
Early online date13 Apr 2005
DOIs
Publication statusPublished - 1 May 2005

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Keywords

  • Animals
  • Brain
  • Cachexia
  • Candida albicans
  • Candidiasis
  • Disease Models, Animal
  • Female
  • Fungemia
  • Kidney
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred DBA
  • Survival Analysis
  • Time Factors
  • Candida albicans
  • mouse model
  • virulence

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