Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes

H. Murakami, S. Keeney

Research output: Contribution to journalArticlepeer-review

Abstract

It has been long appreciated that, during meiosis, DNA replication is coordinated with the subsequent formation of the double-strand breaks (DSBs) that initiate recombination, but a mechanistic understanding of this process was elusive. We now show that, in yeast, the replisome-associated components Tof1 and Csm3 physically associate with the Dbf4-dependent Cdc7 kinase (DDK) and recruit it to the replisome, where it phosphorylates the DSB-promoting factor Mer2 in the wake of the replication fork, synchronizing replication with an early prerequisite for DSB formation. Recruiting regulatory kinases to replisomes may be a general mechanism to ensure spatial and temporal coordination of replication with other chromosomal processes.
Original languageEnglish
Pages (from-to)861-873
JournalCell
Volume158
Issue number4
DOIs
Publication statusPublished - 14 Aug 2014

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