Tetracycline susceptibility of the ingested Lactobacillus acidophilus LaCH-5 and Bifidobacterium animalis subsp lactis Bb-12 strains during antibiotic/probiotic intervention

Maria Saarela, Johanna Maukonen, Atte Von Wright, Terttu Vilpponen-Salmela, Andrea J. Patterson, Karen P. Scott, Heikki Hamynen, Janna Matto

Research output: Contribution to journalArticle

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Abstract

We investigated the effects of oral therapy with doxycycline, a tetracycline group antibiotic, on the gastrointestinal (GI) survival and tetracycline susceptibility of probiotic strains Lactobacillus acidophilus LaCH-5 and Bifidobacterium animalis subsp. lactis Bb-12. In addition, the influence of doxycycline therapy on the diversity of the predominant faecal microbiota was evaluated by polymerase chain reaction--denaturing gradient gel electrophoresis (PCR-DGGE). Faecal samples from the antibiotic group (receiving antibiotics and probiotics) and the control group (receiving probiotics only) were analysed for anaerobically and aerobically growing bacteria, biliclobacteria and lactic acid bacteria as well as for the dominant microbiota. Although doxycycline consumption did not have a large impact on GI survival of the probiotics, it had a detrimental effect on the bifidobacteria and on the diversity of the dominant faecal microbiota. A higher proportion of tetracycline-resistant anaerobically growing bacteria and bifidobacteria was detected in the antibiotic group than in the control group. Several antibiotic group subjects had faecal B. animalis subsp. lactis Bb-12-like isolates with reduced tetracycline susceptibility. This was unlikely to be due to the acquisition of novel tetracycline resistance determinants, since only tet(W), which is also present in the ingested B. animalis subsp. lactis Bb-12, was found in the resistant isolates. Thus, concomitant ingestion of probiotic L. acidophilus LaCH-5 and B. animalis subsp. lactis Bb-12 with the antibiotic did not generate a safety risk regarding the possible GI transfer of tetracycline resistance genes to the ingested strains.

Original languageEnglish
Pages (from-to)271-280
Number of pages10
JournalInternational Journal of Antimicrobial Agents
Volume29
Issue number3
Early online date5 Jan 2007
DOIs
Publication statusPublished - Mar 2007

Keywords

  • tetracycline
  • tet(W)
  • doxycycline
  • susceptibility
  • Lactobacillus acidophilus
  • Bifidobacterium animalis
  • helicobacter-pylori eradication
  • antimicrobial susceptibility
  • intestinal microflora
  • resistance gene
  • double-blind
  • therapy
  • microbiota
  • GG
  • supplementation
  • probiotics

Cite this

Tetracycline susceptibility of the ingested Lactobacillus acidophilus LaCH-5 and Bifidobacterium animalis subsp lactis Bb-12 strains during antibiotic/probiotic intervention. / Saarela, Maria; Maukonen, Johanna; Von Wright, Atte; Vilpponen-Salmela, Terttu; Patterson, Andrea J.; Scott, Karen P.; Hamynen, Heikki; Matto, Janna.

In: International Journal of Antimicrobial Agents, Vol. 29, No. 3, 03.2007, p. 271-280.

Research output: Contribution to journalArticle

Saarela, Maria ; Maukonen, Johanna ; Von Wright, Atte ; Vilpponen-Salmela, Terttu ; Patterson, Andrea J. ; Scott, Karen P. ; Hamynen, Heikki ; Matto, Janna. / Tetracycline susceptibility of the ingested Lactobacillus acidophilus LaCH-5 and Bifidobacterium animalis subsp lactis Bb-12 strains during antibiotic/probiotic intervention. In: International Journal of Antimicrobial Agents. 2007 ; Vol. 29, No. 3. pp. 271-280.
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abstract = "We investigated the effects of oral therapy with doxycycline, a tetracycline group antibiotic, on the gastrointestinal (GI) survival and tetracycline susceptibility of probiotic strains Lactobacillus acidophilus LaCH-5 and Bifidobacterium animalis subsp. lactis Bb-12. In addition, the influence of doxycycline therapy on the diversity of the predominant faecal microbiota was evaluated by polymerase chain reaction--denaturing gradient gel electrophoresis (PCR-DGGE). Faecal samples from the antibiotic group (receiving antibiotics and probiotics) and the control group (receiving probiotics only) were analysed for anaerobically and aerobically growing bacteria, biliclobacteria and lactic acid bacteria as well as for the dominant microbiota. Although doxycycline consumption did not have a large impact on GI survival of the probiotics, it had a detrimental effect on the bifidobacteria and on the diversity of the dominant faecal microbiota. A higher proportion of tetracycline-resistant anaerobically growing bacteria and bifidobacteria was detected in the antibiotic group than in the control group. Several antibiotic group subjects had faecal B. animalis subsp. lactis Bb-12-like isolates with reduced tetracycline susceptibility. This was unlikely to be due to the acquisition of novel tetracycline resistance determinants, since only tet(W), which is also present in the ingested B. animalis subsp. lactis Bb-12, was found in the resistant isolates. Thus, concomitant ingestion of probiotic L. acidophilus LaCH-5 and B. animalis subsp. lactis Bb-12 with the antibiotic did not generate a safety risk regarding the possible GI transfer of tetracycline resistance genes to the ingested strains.",
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author = "Maria Saarela and Johanna Maukonen and {Von Wright}, Atte and Terttu Vilpponen-Salmela and Patterson, {Andrea J.} and Scott, {Karen P.} and Heikki Hamynen and Janna Matto",
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T1 - Tetracycline susceptibility of the ingested Lactobacillus acidophilus LaCH-5 and Bifidobacterium animalis subsp lactis Bb-12 strains during antibiotic/probiotic intervention

AU - Saarela, Maria

AU - Maukonen, Johanna

AU - Von Wright, Atte

AU - Vilpponen-Salmela, Terttu

AU - Patterson, Andrea J.

AU - Scott, Karen P.

AU - Hamynen, Heikki

AU - Matto, Janna

PY - 2007/3

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N2 - We investigated the effects of oral therapy with doxycycline, a tetracycline group antibiotic, on the gastrointestinal (GI) survival and tetracycline susceptibility of probiotic strains Lactobacillus acidophilus LaCH-5 and Bifidobacterium animalis subsp. lactis Bb-12. In addition, the influence of doxycycline therapy on the diversity of the predominant faecal microbiota was evaluated by polymerase chain reaction--denaturing gradient gel electrophoresis (PCR-DGGE). Faecal samples from the antibiotic group (receiving antibiotics and probiotics) and the control group (receiving probiotics only) were analysed for anaerobically and aerobically growing bacteria, biliclobacteria and lactic acid bacteria as well as for the dominant microbiota. Although doxycycline consumption did not have a large impact on GI survival of the probiotics, it had a detrimental effect on the bifidobacteria and on the diversity of the dominant faecal microbiota. A higher proportion of tetracycline-resistant anaerobically growing bacteria and bifidobacteria was detected in the antibiotic group than in the control group. Several antibiotic group subjects had faecal B. animalis subsp. lactis Bb-12-like isolates with reduced tetracycline susceptibility. This was unlikely to be due to the acquisition of novel tetracycline resistance determinants, since only tet(W), which is also present in the ingested B. animalis subsp. lactis Bb-12, was found in the resistant isolates. Thus, concomitant ingestion of probiotic L. acidophilus LaCH-5 and B. animalis subsp. lactis Bb-12 with the antibiotic did not generate a safety risk regarding the possible GI transfer of tetracycline resistance genes to the ingested strains.

AB - We investigated the effects of oral therapy with doxycycline, a tetracycline group antibiotic, on the gastrointestinal (GI) survival and tetracycline susceptibility of probiotic strains Lactobacillus acidophilus LaCH-5 and Bifidobacterium animalis subsp. lactis Bb-12. In addition, the influence of doxycycline therapy on the diversity of the predominant faecal microbiota was evaluated by polymerase chain reaction--denaturing gradient gel electrophoresis (PCR-DGGE). Faecal samples from the antibiotic group (receiving antibiotics and probiotics) and the control group (receiving probiotics only) were analysed for anaerobically and aerobically growing bacteria, biliclobacteria and lactic acid bacteria as well as for the dominant microbiota. Although doxycycline consumption did not have a large impact on GI survival of the probiotics, it had a detrimental effect on the bifidobacteria and on the diversity of the dominant faecal microbiota. A higher proportion of tetracycline-resistant anaerobically growing bacteria and bifidobacteria was detected in the antibiotic group than in the control group. Several antibiotic group subjects had faecal B. animalis subsp. lactis Bb-12-like isolates with reduced tetracycline susceptibility. This was unlikely to be due to the acquisition of novel tetracycline resistance determinants, since only tet(W), which is also present in the ingested B. animalis subsp. lactis Bb-12, was found in the resistant isolates. Thus, concomitant ingestion of probiotic L. acidophilus LaCH-5 and B. animalis subsp. lactis Bb-12 with the antibiotic did not generate a safety risk regarding the possible GI transfer of tetracycline resistance genes to the ingested strains.

KW - tetracycline

KW - tet(W)

KW - doxycycline

KW - susceptibility

KW - Lactobacillus acidophilus

KW - Bifidobacterium animalis

KW - helicobacter-pylori eradication

KW - antimicrobial susceptibility

KW - intestinal microflora

KW - resistance gene

KW - double-blind

KW - therapy

KW - microbiota

KW - GG

KW - supplementation

KW - probiotics

U2 - 10.1016/j.ijantimicag.2006.09.020

DO - 10.1016/j.ijantimicag.2006.09.020

M3 - Article

VL - 29

SP - 271

EP - 280

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

IS - 3

ER -