Abstract
A partial nucleotide sequence of transforming growth factor-beta 3 (TGF-beta 3) has been isolated from the Siberian sturgeon (Acipenser baeri), rainbow trout (Oncorhynchus mykiss) and European eel (Anguilla anguilla), confirming a ubiquitous presence in the ray-finned (Actinopterygian) bony fish. The bony fish TGF-beta 3 is highly conserved, with some 83-84% nucleotide identity (coding region) and 90-95% predicted amino acid identity to known homeotherm TGF-beta 3's. Far lower homologies are apparent with other known TGF-P isoforms in fish (e.g. 64-66% and 81-82% amino acid identity to trout TGF beta 1/5 and carp TGF-beta 2 respectively). Phylogenetic tree analysis showed that the fish TGF-beta 3's clustered with the known homeotherm TGF-beta 3's. The relatively tight clustering of TGF-beta 1, TGF-beta 2 and TGF-beta 3 was in contrast to the TGF-beta 5's, which are clearly a more heterogenous group. (C) 1999 Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 45-53 |
| Number of pages | 9 |
| Journal | Veterinary Immunology and Immunopathology |
| Volume | 72 |
| Publication status | Published - 1999 |
Keywords
- bony fish
- teleosts
- chondrosteans
- TGF-beta 3
- partial gene
- TRANSFORMING GROWTH FACTOR-BETA-5
- TROUT ONCORHYNCHUS-MYKISS
- XENOPUS-LAEVIS
- TGF-BETA
- SEQUENCE
- GENE
- EXPRESSION
- CDNA