Thaumarchaeal ammonia oxidation in an acidic forest peat soil is not influenced by ammonium amendment

Nejc Stopnisek, Cécile Gubry-Rangin, Spela Höfferle, Graeme W Nicol, Ines Mandic-Mulec, James I Prosser

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Both bacteria and thaumarchaea contribute to ammonia oxidation, the first step in nitrification. The abundance of putative ammonia oxidizers is estimated by quantification of the functional gene amoA, which encodes ammonia monooxygenase subunit A. In soil, thaumarchaeal amoA genes often outnumber the equivalent bacterial genes. Ecophysiological studies indicate that thaumarchaeal ammonia oxidizers may have a selective advantage at low ammonia concentrations, with potential adaptation to soils in which mineralization is the major source of ammonia. To test this hypothesis, thaumarchaeal and bacterial ammonia oxidizers were investigated during nitrification in microcosms containing an organic, acidic forest peat soil (pH 4.1) with a low ammonium concentration but high potential for ammonia release during mineralization. Net nitrification rates were high but were not influenced by addition of ammonium. Bacterial amoA genes could not be detected, presumably because of low abundance of bacterial ammonia oxidizers. Phylogenetic analysis of thaumarchaeal 16S rRNA gene sequences indicated that dominant populations belonged to group 1.1c, 1.3, and "deep peat" lineages, while known amo-containing lineages (groups 1.1a and 1.1b) comprised only a small proportion of the total community. Growth of thaumarchaeal ammonia oxidizers was indicated by increased abundance of amoA genes during nitrification but was unaffected by addition of ammonium. Similarly, denaturing gradient gel electrophoresis analysis of amoA gene transcripts demonstrated small temporal changes in thaumarchaeal ammonia oxidizer communities but no effect of ammonium amendment. Thaumarchaea therefore appeared to dominate ammonia oxidation in this soil and oxidized ammonia arising from mineralization of organic matter rather than added inorganic nitrogen.
Original languageEnglish
Pages (from-to)7626-7634
Number of pages9
JournalApplied and Environmental Microbiology
Volume76
Issue number22
Early online date1 Oct 2010
DOIs
Publication statusPublished - Nov 2010

Keywords

  • archaea
  • bacteria
  • cluster analysis
  • DNA, archaeal
  • DNA, bacterial
  • DNA, ribosomal
  • molecular sequence data
  • nitrification
  • oxidation-reduction
  • oxidoreductases
  • phylogeny
  • quaternary ammonium compounds
  • RNA, ribosomal, 16S
  • sequence analysis, DNA
  • soil
  • soil microbiology
  • trees

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