The AMPK system of salmonid fishes was expanded through genome duplication and is regulated by growth and immune status in muscle

Dwight R. Causey, Jin Hyoung Kim, Robert H. Devlin, Samuel A.M. Martin, Daniel J. Macqueen* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

5′adenosine monophosphate-activated protein kinase (AMPK) is a master regulator of energy homeostasis in eukaryotes. This study identified expansions in the AMPK-α, -β and -γ families of salmonid fishes due to a history of genome duplication events, including five novel salmonid-specific AMPK subunit gene paralogue pairs. We tested the hypothesis that the expanded AMPK gene system of salmonids is transcriptionally regulated by growth and immunological status. As a model, we studied immune-stimulated coho salmon (Oncorhynchus kisutch) from three experiment groups sharing the same genetic background, but showing highly-divergent growth rates and nutritional status. Specifically, we compared wild-type and GH-transgenic fish, the latter achieving either enhanced or wild-type growth rate via ration manipulation. Transcript levels for the fifteen unique salmonid AMPK subunit genes were quantified in skeletal muscle after stimulation with bacterial or viral mimics to alter immune status. These analyses revealed a constitutive up-regulation of several AMPK-α and -γ subunit-encoding genes in GH-transgenic fish achieving accelerated growth. Further, immune stimulation caused a decrease in the expression of several AMPK subunit-encoding genes in GH-transgenic fish specifically. The dynamic expression responses observed suggest a role for the AMPK system in balancing energetic investment into muscle growth according to immunological status in salmonid fishes.

Original languageEnglish
Article number9819
Number of pages11
JournalScientific Reports
Volume9
DOIs
Publication statusPublished - 8 Jul 2019

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AMP-Activated Protein Kinases
Fishes
Genome
Muscles
Growth
Oncorhynchus kisutch
Genes
Salmonidae
Eukaryota
Nutritional Status
Protein Kinases
Skeletal Muscle
Homeostasis
Up-Regulation

ASJC Scopus subject areas

  • General

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The AMPK system of salmonid fishes was expanded through genome duplication and is regulated by growth and immune status in muscle. / Causey, Dwight R.; Kim, Jin Hyoung; Devlin, Robert H.; Martin, Samuel A.M.; Macqueen, Daniel J. (Corresponding Author).

In: Scientific Reports, Vol. 9, 9819, 08.07.2019.

Research output: Contribution to journalArticle

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abstract = "5′adenosine monophosphate-activated protein kinase (AMPK) is a master regulator of energy homeostasis in eukaryotes. This study identified expansions in the AMPK-α, -β and -γ families of salmonid fishes due to a history of genome duplication events, including five novel salmonid-specific AMPK subunit gene paralogue pairs. We tested the hypothesis that the expanded AMPK gene system of salmonids is transcriptionally regulated by growth and immunological status. As a model, we studied immune-stimulated coho salmon (Oncorhynchus kisutch) from three experiment groups sharing the same genetic background, but showing highly-divergent growth rates and nutritional status. Specifically, we compared wild-type and GH-transgenic fish, the latter achieving either enhanced or wild-type growth rate via ration manipulation. Transcript levels for the fifteen unique salmonid AMPK subunit genes were quantified in skeletal muscle after stimulation with bacterial or viral mimics to alter immune status. These analyses revealed a constitutive up-regulation of several AMPK-α and -γ subunit-encoding genes in GH-transgenic fish achieving accelerated growth. Further, immune stimulation caused a decrease in the expression of several AMPK subunit-encoding genes in GH-transgenic fish specifically. The dynamic expression responses observed suggest a role for the AMPK system in balancing energetic investment into muscle growth according to immunological status in salmonid fishes.",
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note = "Acknowledgements DRC’s PhD studentship is supported through Te Developmental Trust and Elphinstone Scholarship Programme of the University of Aberdeen. DJM acknowledges support from BBSRC Institute Strategic Programme funding to The Roslin Institute (grant ref: BBS/E/D/10002071).",
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