Abstract
In this report, recombinant interieukin-8 (rIL-8) was produced and its activity tested for the first time in fish. The rainbow trout rIL-8 was produced in Escherichia coli and purified using a 6xHis tag at the N-terminus. The rIL-8 induced a dose-dependent migration of head kidney leukocytes at concentrations from 0.1 to 10 ng/ml, with a peak response at 1 ng/ml. Trout rIL-8 also had a significant effect on superoxide production by head kidney cells, with maximal, activity at 0.1 and 1 ng/ml. When injected intraperitoneally into trout, rIL-8 had a clear effect on total leukocyte number in the peritoneal cavity, with increasing doses (up to 5 mu g) eliciting more cells. Of three leukocyte types distinguished, neutrophils were the dominant cell type, especially at higher rIL-8 concentrations. In contrast, the proportion of macrophages and lymphocytes decreased with rIL-8 administration, suggesting that they were not attracted at the same rate as neutrophils. (C) 2007 Elsevier Ltd. All rights reserved.
Original language | English |
---|---|
Pages (from-to) | 673-681 |
Number of pages | 9 |
Journal | Developmental and Comparative Immunology |
Volume | 32 |
Issue number | 6 |
Early online date | 9 Nov 2007 |
DOIs | |
Publication status | Published - 2008 |
Keywords
- rainbow trout
- recombinant protein
- interleukin-8
- biological activity
- migration
- respiratory burst
- peritoneal-cavity
- functional-characterization
- host defense
- in-vitro
- neutrophil
- cloning
- chemotaxis
- leukocytes
- cytokine