The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8

Nor Omaima Harun, Jun Zou, Yang Yang Zhang, Pin Nie, Christopher John Secombes

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71 Citations (Scopus)

Abstract

In this report, recombinant interieukin-8 (rIL-8) was produced and its activity tested for the first time in fish. The rainbow trout rIL-8 was produced in Escherichia coli and purified using a 6xHis tag at the N-terminus. The rIL-8 induced a dose-dependent migration of head kidney leukocytes at concentrations from 0.1 to 10 ng/ml, with a peak response at 1 ng/ml. Trout rIL-8 also had a significant effect on superoxide production by head kidney cells, with maximal, activity at 0.1 and 1 ng/ml. When injected intraperitoneally into trout, rIL-8 had a clear effect on total leukocyte number in the peritoneal cavity, with increasing doses (up to 5 mu g) eliciting more cells. Of three leukocyte types distinguished, neutrophils were the dominant cell type, especially at higher rIL-8 concentrations. In contrast, the proportion of macrophages and lymphocytes decreased with rIL-8 administration, suggesting that they were not attracted at the same rate as neutrophils. (C) 2007 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)673-681
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume32
Issue number6
Early online date9 Nov 2007
DOIs
Publication statusPublished - 2008

Keywords

  • rainbow trout
  • recombinant protein
  • interleukin-8
  • biological activity
  • migration
  • respiratory burst
  • peritoneal-cavity
  • functional-characterization
  • host defense
  • in-vitro
  • neutrophil
  • cloning
  • chemotaxis
  • leukocytes
  • cytokine

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