The catalytic center in nitrous oxide reductase, Cu-z, is a copper-sulfide cluster

Tim Rasmussen, B C Berks, J Sanders-Loehr, D M Dooley, W G Zumft, A J Thomson

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

The crystal structure of nitrous oxide reductase, the enzyme catalyzing the final step of bacterial denitrification in which nitrous oxide is reduced to dinitrogen, exhibits a novel catalytic site, called Cu-z. This comprises a cluster of four copper ions bound by seven histidines and three other ligands modeled in the X-ray structure as OH- or H2O. However, elemental analyses and resonance Raman spectroscopy of isotopically labeled enzyme conclusively demonstrate that Cu-z has one acid-labile sulfur ligand. Thus, nitrous oxide reductase contains the first reported biological copper-sulfide cluster.

Original languageEnglish
Pages (from-to)12753-12756
Number of pages4
JournalBiochemistry
Volume39
Issue number42
DOIs
Publication statusPublished - 24 Oct 2000

Keywords

  • ELECTRON-TRANSFER CENTER
  • PSEUDOMONAS-PERFECTOMARINA
  • PARACOCCUS-DENITRIFICANS
  • MULTICOPPER ENZYME
  • A CENTER
  • PURIFICATION
  • SPECTROSCOPY
  • PROTEINS
  • STUTZERI
  • SULFUR
  • ELECTRON TRANSFER CENTER

Cite this

Rasmussen, T., Berks, B. C., Sanders-Loehr, J., Dooley, D. M., Zumft, W. G., & Thomson, A. J. (2000). The catalytic center in nitrous oxide reductase, Cu-z, is a copper-sulfide cluster. Biochemistry, 39(42), 12753-12756. https://doi.org/10.1021/bi001811i

The catalytic center in nitrous oxide reductase, Cu-z, is a copper-sulfide cluster. / Rasmussen, Tim; Berks, B C ; Sanders-Loehr, J ; Dooley, D M ; Zumft, W G ; Thomson, A J .

In: Biochemistry, Vol. 39, No. 42, 24.10.2000, p. 12753-12756.

Research output: Contribution to journalArticle

Rasmussen, T, Berks, BC, Sanders-Loehr, J, Dooley, DM, Zumft, WG & Thomson, AJ 2000, 'The catalytic center in nitrous oxide reductase, Cu-z, is a copper-sulfide cluster', Biochemistry, vol. 39, no. 42, pp. 12753-12756. https://doi.org/10.1021/bi001811i
Rasmussen T, Berks BC, Sanders-Loehr J, Dooley DM, Zumft WG, Thomson AJ. The catalytic center in nitrous oxide reductase, Cu-z, is a copper-sulfide cluster. Biochemistry. 2000 Oct 24;39(42):12753-12756. https://doi.org/10.1021/bi001811i
Rasmussen, Tim ; Berks, B C ; Sanders-Loehr, J ; Dooley, D M ; Zumft, W G ; Thomson, A J . / The catalytic center in nitrous oxide reductase, Cu-z, is a copper-sulfide cluster. In: Biochemistry. 2000 ; Vol. 39, No. 42. pp. 12753-12756.
@article{b664591290744bcea8b9bc52e4615505,
title = "The catalytic center in nitrous oxide reductase, Cu-z, is a copper-sulfide cluster",
abstract = "The crystal structure of nitrous oxide reductase, the enzyme catalyzing the final step of bacterial denitrification in which nitrous oxide is reduced to dinitrogen, exhibits a novel catalytic site, called Cu-z. This comprises a cluster of four copper ions bound by seven histidines and three other ligands modeled in the X-ray structure as OH- or H2O. However, elemental analyses and resonance Raman spectroscopy of isotopically labeled enzyme conclusively demonstrate that Cu-z has one acid-labile sulfur ligand. Thus, nitrous oxide reductase contains the first reported biological copper-sulfide cluster.",
keywords = "ELECTRON-TRANSFER CENTER, PSEUDOMONAS-PERFECTOMARINA, PARACOCCUS-DENITRIFICANS, MULTICOPPER ENZYME, A CENTER, PURIFICATION, SPECTROSCOPY, PROTEINS, STUTZERI, SULFUR, ELECTRON TRANSFER CENTER",
author = "Tim Rasmussen and Berks, {B C} and J Sanders-Loehr and Dooley, {D M} and Zumft, {W G} and Thomson, {A J}",
year = "2000",
month = "10",
day = "24",
doi = "10.1021/bi001811i",
language = "English",
volume = "39",
pages = "12753--12756",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "42",

}

TY - JOUR

T1 - The catalytic center in nitrous oxide reductase, Cu-z, is a copper-sulfide cluster

AU - Rasmussen, Tim

AU - Berks, B C

AU - Sanders-Loehr, J

AU - Dooley, D M

AU - Zumft, W G

AU - Thomson, A J

PY - 2000/10/24

Y1 - 2000/10/24

N2 - The crystal structure of nitrous oxide reductase, the enzyme catalyzing the final step of bacterial denitrification in which nitrous oxide is reduced to dinitrogen, exhibits a novel catalytic site, called Cu-z. This comprises a cluster of four copper ions bound by seven histidines and three other ligands modeled in the X-ray structure as OH- or H2O. However, elemental analyses and resonance Raman spectroscopy of isotopically labeled enzyme conclusively demonstrate that Cu-z has one acid-labile sulfur ligand. Thus, nitrous oxide reductase contains the first reported biological copper-sulfide cluster.

AB - The crystal structure of nitrous oxide reductase, the enzyme catalyzing the final step of bacterial denitrification in which nitrous oxide is reduced to dinitrogen, exhibits a novel catalytic site, called Cu-z. This comprises a cluster of four copper ions bound by seven histidines and three other ligands modeled in the X-ray structure as OH- or H2O. However, elemental analyses and resonance Raman spectroscopy of isotopically labeled enzyme conclusively demonstrate that Cu-z has one acid-labile sulfur ligand. Thus, nitrous oxide reductase contains the first reported biological copper-sulfide cluster.

KW - ELECTRON-TRANSFER CENTER

KW - PSEUDOMONAS-PERFECTOMARINA

KW - PARACOCCUS-DENITRIFICANS

KW - MULTICOPPER ENZYME

KW - A CENTER

KW - PURIFICATION

KW - SPECTROSCOPY

KW - PROTEINS

KW - STUTZERI

KW - SULFUR

KW - ELECTRON TRANSFER CENTER

U2 - 10.1021/bi001811i

DO - 10.1021/bi001811i

M3 - Article

VL - 39

SP - 12753

EP - 12756

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 42

ER -