The CD34 surface antigen is restricted to glucagon-expressing cells in the early developing bovine pancreas

Claudia Merkwitz, Tiina Pessa-Morikawa, Paul Lochhead, Gessner Reinhard, Michiharu Sakurai, Antti Iivanainen, Albert M Ricken

Research output: Contribution to journalArticle

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Abstract

Controversy remains regarding the origin of the pancreatic endocrine cells. It is generally accepted that the majority of insulin-secreting cells derive from the endodermal epithelium of the gastrointestinal tract. The aim of this study was to determine the contribution made by a particular cluster of differentiation (CD)-positive cells to the development of the bovine endocrine pancreas. In bovine embryos and foetuses with crown to rump lengths (CRL) ranging from 1 to 47 cm, cells staining positively for CD34 and/or CD133 were always more numerous in the left lobe and body of pancreas than in the right lobe. In the early stages of pancreatic development (CRL 5 cm), individual or groups of CD34 and/or CD133-reactive cells were present in newly formed acini, which bulged out from the duct system that had arisen from the cords. Some of the positively stained cells accumulated in focal areas associated with hyperplastic intra-acinar cells. These "acino-insula-like complexes" appeared to enlarge with age and develop into intralobular Islets of Langerhans. Most of the described CD34 and/or CD133-reactive cells displayed co-localisation with glucagon. A negligible number of these cells showed co-localisation with insulin. Glucagon-stained cells were distinct from insulin-stained cells and were more abundant in embryonic and early foetal pancreata. Our data demonstrate that CD34 and/or CD133-reactive cells contribute to the pancreatic alpha cell population during early foetal development in cattle.
Original languageEnglish
Pages (from-to)59-71
Number of pages13
JournalHistochemistry and Cell Biology
Volume135
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

CD34 Antigens
Surface Antigens
Glucagon
Pancreas
Crown-Rump Length
Islets of Langerhans
Glucagon-Secreting Cells
Insulin
Endocrine Cells
Acinar Cells
Insulin-Secreting Cells
Fetal Development
Gastrointestinal Tract
Cell Differentiation
Fetus
Embryonic Structures
Epithelium
Cell Count
Staining and Labeling

Keywords

  • animals
  • antigens, CD34
  • cattle
  • fetus
  • glucagon
  • immunohistochemistry
  • pancreas
  • CD34
  • CD133
  • progenitor cells
  • endocrine cells
  • alpha cells
  • development
  • foetus

Cite this

Merkwitz, C., Pessa-Morikawa, T., Lochhead, P., Reinhard, G., Sakurai, M., Iivanainen, A., & Ricken, A. M. (2011). The CD34 surface antigen is restricted to glucagon-expressing cells in the early developing bovine pancreas. Histochemistry and Cell Biology, 135(1), 59-71. https://doi.org/10.1007/s00418-010-0775-x

The CD34 surface antigen is restricted to glucagon-expressing cells in the early developing bovine pancreas. / Merkwitz, Claudia; Pessa-Morikawa, Tiina; Lochhead, Paul; Reinhard, Gessner; Sakurai, Michiharu; Iivanainen, Antti; Ricken, Albert M.

In: Histochemistry and Cell Biology, Vol. 135, No. 1, 01.2011, p. 59-71.

Research output: Contribution to journalArticle

Merkwitz, C, Pessa-Morikawa, T, Lochhead, P, Reinhard, G, Sakurai, M, Iivanainen, A & Ricken, AM 2011, 'The CD34 surface antigen is restricted to glucagon-expressing cells in the early developing bovine pancreas', Histochemistry and Cell Biology, vol. 135, no. 1, pp. 59-71. https://doi.org/10.1007/s00418-010-0775-x
Merkwitz, Claudia ; Pessa-Morikawa, Tiina ; Lochhead, Paul ; Reinhard, Gessner ; Sakurai, Michiharu ; Iivanainen, Antti ; Ricken, Albert M. / The CD34 surface antigen is restricted to glucagon-expressing cells in the early developing bovine pancreas. In: Histochemistry and Cell Biology. 2011 ; Vol. 135, No. 1. pp. 59-71.
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