The coffee diterpene cafestol increases plasma triacylglycerol by increasing the production rate of large VLDL apolipoprotein B in healthy normolipidemic subjects

B de Roos, M J Caslake, A F Stalenhoef, D Bedford, P N Demacker, M B Katan, C J Packard

    Research output: Contribution to journalArticle

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    Abstract

    Background: Cafestol is a diterpene in unfiltered coffee that raises plasma triacylglycerol in humans.

    Objective: We studied whether cafestol increases plasma triacylglycerol by increasing the production rate or by decreasing the fractional catabolic rate of VLDL1 [Svedberg flotation unit (Sf) 60–400] apolipoprotein (apo) B. In addition, we studied the effect of cafestol on the composition of VLDL1 and VLDL2 (Sf 20–60).

    Design: Eight healthy normolipidemic men were administered a daily dose of 75 mg cafestol for 2 wk. A bolus injection of 7 mg L-[5,5,5-2H3]leucine/kg body wt was given after a baseline period with no cafestol and again after treatment with cafestol. We derived kinetic constants to describe the metabolism of VLDL1 apo B by using a multicompartmental model.

    Results: Cafestol significantly increased plasma triacylglycerol by 31% or 0.32 mmol/L (95% CI: 0.03, 0.61); the increase was due mainly to a nonsignificant rise in VLDL1 triacylglycerol of 57% or 0.23 mmol/L (95% CI: −0.02, 0.48). Cafestol significantly increased the mean rate of VLDL1 apo B production by 80% or 755 mg/d (95% CI: 0.2, 5353), whereas it did not significantly change the mean fractional catabolic rate of VLDL1 apo B (mean increase of 3 pools/d; 95% CI: −4, 10]). Cafestol did not change the composition of VLDL1. A significant increase in the ratio of VLDL2 cholesteryl ester to triacylglycerol indicates that VLDL2 became enriched with cholesteryl esters at the cost of triacylglycerol.

    Conclusion: Cafestol increases plasma triacylglycerol by increasing the production rate of VLDL1 apo B, probably via increased assembly of VLDL1 in the liver.
    Original languageEnglish
    Pages (from-to)45-52
    Number of pages8
    JournalThe American Journal of Clinical Nutrition
    Volume73
    Issue number1
    Publication statusPublished - Jan 2001

    Fingerprint

    Diterpenes
    Coffee
    Apolipoproteins B
    Healthy Volunteers
    Triglycerides
    Cholesterol Esters
    cafestol
    Leucine
    Injections

    Keywords

    • healthy men
    • cafestol
    • VLDL1 apolipoprotein B
    • catabolism
    • VLDL composition
    • unfiltered coffee
    • cafetiere coffee
    • French-press coffee
    • triacylglycerol
    • triglyceride

    Cite this

    The coffee diterpene cafestol increases plasma triacylglycerol by increasing the production rate of large VLDL apolipoprotein B in healthy normolipidemic subjects. / de Roos, B; Caslake, M J; Stalenhoef, A F; Bedford, D; Demacker, P N; Katan, M B; Packard, C J.

    In: The American Journal of Clinical Nutrition, Vol. 73, No. 1, 01.2001, p. 45-52.

    Research output: Contribution to journalArticle

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    abstract = "Background: Cafestol is a diterpene in unfiltered coffee that raises plasma triacylglycerol in humans.Objective: We studied whether cafestol increases plasma triacylglycerol by increasing the production rate or by decreasing the fractional catabolic rate of VLDL1 [Svedberg flotation unit (Sf) 60–400] apolipoprotein (apo) B. In addition, we studied the effect of cafestol on the composition of VLDL1 and VLDL2 (Sf 20–60).Design: Eight healthy normolipidemic men were administered a daily dose of 75 mg cafestol for 2 wk. A bolus injection of 7 mg L-[5,5,5-2H3]leucine/kg body wt was given after a baseline period with no cafestol and again after treatment with cafestol. We derived kinetic constants to describe the metabolism of VLDL1 apo B by using a multicompartmental model.Results: Cafestol significantly increased plasma triacylglycerol by 31{\%} or 0.32 mmol/L (95{\%} CI: 0.03, 0.61); the increase was due mainly to a nonsignificant rise in VLDL1 triacylglycerol of 57{\%} or 0.23 mmol/L (95{\%} CI: −0.02, 0.48). Cafestol significantly increased the mean rate of VLDL1 apo B production by 80{\%} or 755 mg/d (95{\%} CI: 0.2, 5353), whereas it did not significantly change the mean fractional catabolic rate of VLDL1 apo B (mean increase of 3 pools/d; 95{\%} CI: −4, 10]). Cafestol did not change the composition of VLDL1. A significant increase in the ratio of VLDL2 cholesteryl ester to triacylglycerol indicates that VLDL2 became enriched with cholesteryl esters at the cost of triacylglycerol.Conclusion: Cafestol increases plasma triacylglycerol by increasing the production rate of VLDL1 apo B, probably via increased assembly of VLDL1 in the liver.",
    keywords = "healthy men, cafestol, VLDL1 apolipoprotein B , catabolism, VLDL composition, unfiltered coffee, cafetiere coffee, French-press coffee, triacylglycerol , triglyceride",
    author = "{de Roos}, B and Caslake, {M J} and Stalenhoef, {A F} and D Bedford and Demacker, {P N} and Katan, {M B} and Packard, {C J}",
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    TY - JOUR

    T1 - The coffee diterpene cafestol increases plasma triacylglycerol by increasing the production rate of large VLDL apolipoprotein B in healthy normolipidemic subjects

    AU - de Roos, B

    AU - Caslake, M J

    AU - Stalenhoef, A F

    AU - Bedford, D

    AU - Demacker, P N

    AU - Katan, M B

    AU - Packard, C J

    PY - 2001/1

    Y1 - 2001/1

    N2 - Background: Cafestol is a diterpene in unfiltered coffee that raises plasma triacylglycerol in humans.Objective: We studied whether cafestol increases plasma triacylglycerol by increasing the production rate or by decreasing the fractional catabolic rate of VLDL1 [Svedberg flotation unit (Sf) 60–400] apolipoprotein (apo) B. In addition, we studied the effect of cafestol on the composition of VLDL1 and VLDL2 (Sf 20–60).Design: Eight healthy normolipidemic men were administered a daily dose of 75 mg cafestol for 2 wk. A bolus injection of 7 mg L-[5,5,5-2H3]leucine/kg body wt was given after a baseline period with no cafestol and again after treatment with cafestol. We derived kinetic constants to describe the metabolism of VLDL1 apo B by using a multicompartmental model.Results: Cafestol significantly increased plasma triacylglycerol by 31% or 0.32 mmol/L (95% CI: 0.03, 0.61); the increase was due mainly to a nonsignificant rise in VLDL1 triacylglycerol of 57% or 0.23 mmol/L (95% CI: −0.02, 0.48). Cafestol significantly increased the mean rate of VLDL1 apo B production by 80% or 755 mg/d (95% CI: 0.2, 5353), whereas it did not significantly change the mean fractional catabolic rate of VLDL1 apo B (mean increase of 3 pools/d; 95% CI: −4, 10]). Cafestol did not change the composition of VLDL1. A significant increase in the ratio of VLDL2 cholesteryl ester to triacylglycerol indicates that VLDL2 became enriched with cholesteryl esters at the cost of triacylglycerol.Conclusion: Cafestol increases plasma triacylglycerol by increasing the production rate of VLDL1 apo B, probably via increased assembly of VLDL1 in the liver.

    AB - Background: Cafestol is a diterpene in unfiltered coffee that raises plasma triacylglycerol in humans.Objective: We studied whether cafestol increases plasma triacylglycerol by increasing the production rate or by decreasing the fractional catabolic rate of VLDL1 [Svedberg flotation unit (Sf) 60–400] apolipoprotein (apo) B. In addition, we studied the effect of cafestol on the composition of VLDL1 and VLDL2 (Sf 20–60).Design: Eight healthy normolipidemic men were administered a daily dose of 75 mg cafestol for 2 wk. A bolus injection of 7 mg L-[5,5,5-2H3]leucine/kg body wt was given after a baseline period with no cafestol and again after treatment with cafestol. We derived kinetic constants to describe the metabolism of VLDL1 apo B by using a multicompartmental model.Results: Cafestol significantly increased plasma triacylglycerol by 31% or 0.32 mmol/L (95% CI: 0.03, 0.61); the increase was due mainly to a nonsignificant rise in VLDL1 triacylglycerol of 57% or 0.23 mmol/L (95% CI: −0.02, 0.48). Cafestol significantly increased the mean rate of VLDL1 apo B production by 80% or 755 mg/d (95% CI: 0.2, 5353), whereas it did not significantly change the mean fractional catabolic rate of VLDL1 apo B (mean increase of 3 pools/d; 95% CI: −4, 10]). Cafestol did not change the composition of VLDL1. A significant increase in the ratio of VLDL2 cholesteryl ester to triacylglycerol indicates that VLDL2 became enriched with cholesteryl esters at the cost of triacylglycerol.Conclusion: Cafestol increases plasma triacylglycerol by increasing the production rate of VLDL1 apo B, probably via increased assembly of VLDL1 in the liver.

    KW - healthy men

    KW - cafestol

    KW - VLDL1 apolipoprotein B

    KW - catabolism

    KW - VLDL composition

    KW - unfiltered coffee

    KW - cafetiere coffee

    KW - French-press coffee

    KW - triacylglycerol

    KW - triglyceride

    M3 - Article

    C2 - 11124749

    VL - 73

    SP - 45

    EP - 52

    JO - The American Journal of Clinical Nutrition

    JF - The American Journal of Clinical Nutrition

    SN - 0002-9165

    IS - 1

    ER -