The colonic metabolites dihydrocaffeic acid and dihydroferulic acid are more effective inhibitors of in vitro platelet activation than their phenolic precursors

Gema Baeza, Eva-Maria Bachmair, Sharon Wood, Raquel Mateos, Laura Bravo, Baukje De Roos

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Abstract

Cardiovascular diseases (CVD) are the major cause of morbidity and mortality worldwide. The consumption of healthy diets rich in polyphenols has been inversely associated with the development of CVD. This study evaluated the effects of green coffee bean (GCBE) and yerba mate (YMPE) phenolic extracts, the main phenolic and methylxanthines constituents (5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, caffeine, and theobromine), and their main metabolites (caffeic acid, ferulic acid, dihydrocaffeic acid -DHCA- and dihydroferulic acid -DHFA-) on platelet activation in vitro. Upon incubation with different doses (0.01 – 100 µg/mL or µM) of each compound, adenosine 5’-diphosphate-induced P-selectin expression and fibrinogen binding were determined using whole blood flow cytometry. Platelet P-selectin expression was significantly decreased by YMPE and all phenolic and methylxanthines constituents at physiological concentrations, compared with control, whereas fibrinogen binding on platelets was significantly increased. The colonic metabolites (DHCA and DHFA) had stronger inhibitory effects on P-selectin expression than their phenolic precursors, suggesting an increase in the efficacy to modulate platelet activation with the metabolism of the phenolic compounds.
Original languageEnglish
Pages (from-to)1333-1342
Number of pages10
JournalFood & Function
Volume8
Issue number3
Early online date9 Feb 2017
DOIs
Publication statusPublished - 2017

Fingerprint

platelet activation
P-Selectin
Platelet Activation
ferulic acid
fibrinogen
metabolites
Fibrinogen
cardiovascular diseases
acids
Ilex paraguariensis
Cardiovascular Diseases
Blood Platelets
yerba mate
Theobromine
theobromine
adenosine diphosphate
coffee beans
healthy diet
Coffee
Polyphenols

Keywords

  • green coffee
  • yerba mate
  • phenolic compounds
  • methylxanthines
  • metabolites
  • platelet activation

Cite this

@article{bbf24ddac3b54334a3a87d4cc94c2e67,
title = "The colonic metabolites dihydrocaffeic acid and dihydroferulic acid are more effective inhibitors of in vitro platelet activation than their phenolic precursors",
abstract = "Cardiovascular diseases (CVD) are the major cause of morbidity and mortality worldwide. The consumption of healthy diets rich in polyphenols has been inversely associated with the development of CVD. This study evaluated the effects of green coffee bean (GCBE) and yerba mate (YMPE) phenolic extracts, the main phenolic and methylxanthines constituents (5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, caffeine, and theobromine), and their main metabolites (caffeic acid, ferulic acid, dihydrocaffeic acid -DHCA- and dihydroferulic acid -DHFA-) on platelet activation in vitro. Upon incubation with different doses (0.01 – 100 µg/mL or µM) of each compound, adenosine 5’-diphosphate-induced P-selectin expression and fibrinogen binding were determined using whole blood flow cytometry. Platelet P-selectin expression was significantly decreased by YMPE and all phenolic and methylxanthines constituents at physiological concentrations, compared with control, whereas fibrinogen binding on platelets was significantly increased. The colonic metabolites (DHCA and DHFA) had stronger inhibitory effects on P-selectin expression than their phenolic precursors, suggesting an increase in the efficacy to modulate platelet activation with the metabolism of the phenolic compounds.",
keywords = "green coffee, yerba mate, phenolic compounds, methylxanthines, metabolites, platelet activation",
author = "Gema Baeza and Eva-Maria Bachmair and Sharon Wood and Raquel Mateos and Laura Bravo and {De Roos}, Baukje",
note = "This work was funded by the Spanish Ministry of Economy and Competitivity (projects AGL2010- 18269 and AGL 2015-69986-R). G.B. is a FPI fellow (BES-2011-047476) granted with a bursary for short stays from MINECO (EEBB-I-14-08802). The Rowett Institute of Nutrition and Health receives funding from the Scottish Government Rural and Environment Science and Analytical Services (RESAS). The funding bodies had no involvement in the design and execution of the study.",
year = "2017",
doi = "10.1039/C6FO01404F",
language = "English",
volume = "8",
pages = "1333--1342",
journal = "Food & Function",
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publisher = "Royal Society of Chemistry",
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TY - JOUR

T1 - The colonic metabolites dihydrocaffeic acid and dihydroferulic acid are more effective inhibitors of in vitro platelet activation than their phenolic precursors

AU - Baeza, Gema

AU - Bachmair, Eva-Maria

AU - Wood, Sharon

AU - Mateos, Raquel

AU - Bravo, Laura

AU - De Roos, Baukje

N1 - This work was funded by the Spanish Ministry of Economy and Competitivity (projects AGL2010- 18269 and AGL 2015-69986-R). G.B. is a FPI fellow (BES-2011-047476) granted with a bursary for short stays from MINECO (EEBB-I-14-08802). The Rowett Institute of Nutrition and Health receives funding from the Scottish Government Rural and Environment Science and Analytical Services (RESAS). The funding bodies had no involvement in the design and execution of the study.

PY - 2017

Y1 - 2017

N2 - Cardiovascular diseases (CVD) are the major cause of morbidity and mortality worldwide. The consumption of healthy diets rich in polyphenols has been inversely associated with the development of CVD. This study evaluated the effects of green coffee bean (GCBE) and yerba mate (YMPE) phenolic extracts, the main phenolic and methylxanthines constituents (5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, caffeine, and theobromine), and their main metabolites (caffeic acid, ferulic acid, dihydrocaffeic acid -DHCA- and dihydroferulic acid -DHFA-) on platelet activation in vitro. Upon incubation with different doses (0.01 – 100 µg/mL or µM) of each compound, adenosine 5’-diphosphate-induced P-selectin expression and fibrinogen binding were determined using whole blood flow cytometry. Platelet P-selectin expression was significantly decreased by YMPE and all phenolic and methylxanthines constituents at physiological concentrations, compared with control, whereas fibrinogen binding on platelets was significantly increased. The colonic metabolites (DHCA and DHFA) had stronger inhibitory effects on P-selectin expression than their phenolic precursors, suggesting an increase in the efficacy to modulate platelet activation with the metabolism of the phenolic compounds.

AB - Cardiovascular diseases (CVD) are the major cause of morbidity and mortality worldwide. The consumption of healthy diets rich in polyphenols has been inversely associated with the development of CVD. This study evaluated the effects of green coffee bean (GCBE) and yerba mate (YMPE) phenolic extracts, the main phenolic and methylxanthines constituents (5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, caffeine, and theobromine), and their main metabolites (caffeic acid, ferulic acid, dihydrocaffeic acid -DHCA- and dihydroferulic acid -DHFA-) on platelet activation in vitro. Upon incubation with different doses (0.01 – 100 µg/mL or µM) of each compound, adenosine 5’-diphosphate-induced P-selectin expression and fibrinogen binding were determined using whole blood flow cytometry. Platelet P-selectin expression was significantly decreased by YMPE and all phenolic and methylxanthines constituents at physiological concentrations, compared with control, whereas fibrinogen binding on platelets was significantly increased. The colonic metabolites (DHCA and DHFA) had stronger inhibitory effects on P-selectin expression than their phenolic precursors, suggesting an increase in the efficacy to modulate platelet activation with the metabolism of the phenolic compounds.

KW - green coffee

KW - yerba mate

KW - phenolic compounds

KW - methylxanthines

KW - metabolites

KW - platelet activation

U2 - 10.1039/C6FO01404F

DO - 10.1039/C6FO01404F

M3 - Article

VL - 8

SP - 1333

EP - 1342

JO - Food & Function

JF - Food & Function

SN - 2042-6496

IS - 3

ER -