The complete genome sequence of the Streptomyces temperate phage phi C31: evolutionary relationships to other viruses

Margaret Caroline MacHin Smith, R N Burns, S E Wilson, M A Gregory

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The completed genome sequence of the temperate Streptomyces phage phi C31 is reported. phi C31 contains genes that are related by sequence similarities to several other dsDNA phages infecting many diverse bacterial hosts, including Escherichia, Arthrobacter, Mycobacterium Rhodobacter, Staphylococcus, Bacillus, Streptococcus, Lactobacillus and Lactococcus, These observations provide further evidence that dsDNA phages from diverse bacterial hosts are related and have had access to a common genetic pool, Analysis of the late genes was particularly informative. The sequences of the head assembly proteins (portal, head protease and major capsid) were conserved between phi C31, coliphage HK97, staphylococcal phage phi PVL, two Rhodobacter capsulatus prophages and two Mycobacterium tuberculosis prophages, These phages and prophages (where non-defective) from evolutionarily diverse hosts are, therefore, likely to share a common head assembly mechanism i.e. that of HK97, The organisation of the tail genes in phi C31 is highly reminiscent of tail regions from other phage genomes. The unusual organisation of the putative lysis genes in phi C31 is discussed, and speculations are made as to the roles of some inessential early gene products. Similarities between certain phage gene products and eukaryotic dsDNA virus proteins were noted, in particular, the primase/helicases and the terminases (large subunits), Furthermore, the complete sequence clarifies the overall transcription map of the phage during lytic growth and the positions of elements involved in the maintenance of lysogeny.

Original languageEnglish
Pages (from-to)2145-2155
Number of pages11
JournalNucleic Acids Research
Volume27
Issue number10
Publication statusPublished - 15 May 1999

Keywords

  • PLASMID CLONING VECTORS
  • EARLY REGION
  • LYTIC DEVELOPMENT
  • BACTERIOPHAGE-LAMBDA
  • NUCLEOTIDE-SEQUENCE
  • ESCHERICHIA-COLI
  • DNA-SEQUENCE
  • REPRESSOR
  • PROTEIN
  • ACTINOPHAGE-PHI-C31

Cite this

Smith, M. C. M., Burns, R. N., Wilson, S. E., & Gregory, M. A. (1999). The complete genome sequence of the Streptomyces temperate phage phi C31: evolutionary relationships to other viruses. Nucleic Acids Research, 27(10), 2145-2155.

The complete genome sequence of the Streptomyces temperate phage phi C31: evolutionary relationships to other viruses. / Smith, Margaret Caroline MacHin; Burns, R N ; Wilson, S E ; Gregory, M A .

In: Nucleic Acids Research, Vol. 27, No. 10, 15.05.1999, p. 2145-2155.

Research output: Contribution to journalArticle

Smith, Margaret Caroline MacHin ; Burns, R N ; Wilson, S E ; Gregory, M A . / The complete genome sequence of the Streptomyces temperate phage phi C31: evolutionary relationships to other viruses. In: Nucleic Acids Research. 1999 ; Vol. 27, No. 10. pp. 2145-2155.
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N2 - The completed genome sequence of the temperate Streptomyces phage phi C31 is reported. phi C31 contains genes that are related by sequence similarities to several other dsDNA phages infecting many diverse bacterial hosts, including Escherichia, Arthrobacter, Mycobacterium Rhodobacter, Staphylococcus, Bacillus, Streptococcus, Lactobacillus and Lactococcus, These observations provide further evidence that dsDNA phages from diverse bacterial hosts are related and have had access to a common genetic pool, Analysis of the late genes was particularly informative. The sequences of the head assembly proteins (portal, head protease and major capsid) were conserved between phi C31, coliphage HK97, staphylococcal phage phi PVL, two Rhodobacter capsulatus prophages and two Mycobacterium tuberculosis prophages, These phages and prophages (where non-defective) from evolutionarily diverse hosts are, therefore, likely to share a common head assembly mechanism i.e. that of HK97, The organisation of the tail genes in phi C31 is highly reminiscent of tail regions from other phage genomes. The unusual organisation of the putative lysis genes in phi C31 is discussed, and speculations are made as to the roles of some inessential early gene products. Similarities between certain phage gene products and eukaryotic dsDNA virus proteins were noted, in particular, the primase/helicases and the terminases (large subunits), Furthermore, the complete sequence clarifies the overall transcription map of the phage during lytic growth and the positions of elements involved in the maintenance of lysogeny.

AB - The completed genome sequence of the temperate Streptomyces phage phi C31 is reported. phi C31 contains genes that are related by sequence similarities to several other dsDNA phages infecting many diverse bacterial hosts, including Escherichia, Arthrobacter, Mycobacterium Rhodobacter, Staphylococcus, Bacillus, Streptococcus, Lactobacillus and Lactococcus, These observations provide further evidence that dsDNA phages from diverse bacterial hosts are related and have had access to a common genetic pool, Analysis of the late genes was particularly informative. The sequences of the head assembly proteins (portal, head protease and major capsid) were conserved between phi C31, coliphage HK97, staphylococcal phage phi PVL, two Rhodobacter capsulatus prophages and two Mycobacterium tuberculosis prophages, These phages and prophages (where non-defective) from evolutionarily diverse hosts are, therefore, likely to share a common head assembly mechanism i.e. that of HK97, The organisation of the tail genes in phi C31 is highly reminiscent of tail regions from other phage genomes. The unusual organisation of the putative lysis genes in phi C31 is discussed, and speculations are made as to the roles of some inessential early gene products. Similarities between certain phage gene products and eukaryotic dsDNA virus proteins were noted, in particular, the primase/helicases and the terminases (large subunits), Furthermore, the complete sequence clarifies the overall transcription map of the phage during lytic growth and the positions of elements involved in the maintenance of lysogeny.

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KW - LYTIC DEVELOPMENT

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KW - NUCLEOTIDE-SEQUENCE

KW - ESCHERICHIA-COLI

KW - DNA-SEQUENCE

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