The conceptus induces a switch in protein expression and activities of superoxide dismutase 1 and 2 in the sheep endometrium during early pregnancy

K. H. Al-Gubory; C. Garrel; N. Sugino; P. A. Fowler

doi:10.1016/j.smallrumres.2016.07.006

The conceptus induces a switch in protein expression and activities of superoxide dismutase 1 and 2 in the sheep endometrium during early pregnancy

K. H. Al-Gubory^*, C. Garrel, N. Sugino, P. A. Fowler

^*Corresponding author for this work

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Abstract

There has been a growing interest in the importance of superoxide dismutases (SODs) in the regulation of endometrial function. However, little is known about endometrial SOD1 and SOD2 protein expression and activity associated with early conceptus development. We aimed to investigate changes in protein levels and activities of SOD1 and SOD2 in the sheep caruncular (CAR) and intercaruncular (ICAR) endometrium during the transition from pre-implantation (day 14) to implantation (day 16) and post-implantation (day 18) periods of pregnancy. Lipid peroxidation was assessed by measuring CAR and ICAR malondialdehyde (MDA) content. SOD1 activity increased from day 14 to day 18 (P <0.05) in CAR and from day 14 to day 18 (P <0.05) and from day 16 to day 18 (P <0.01) in ICAR. SOD1 protein level increased from day 16 to day 18 (P <0.05) in CAR and from days 14 to days 16 and 18 (P <0.05) in ICAR. SOD2 activity increased from day 16 to day 18 (P <0.05) in CAR and from days 14 and 16 to day 18 (P <0.001) in ICAR. SOD2 protein level increased from day 14 to day 18 (P <0.05) in CAR and from days 14 and 16 to day 18 (P <0.05) in ICAR. The content of MDA in CAR or ICAR did not alter significantly between stages of pregnancy. In conclusion, the early post-implanting conceptus co-ordinately up-regulates SOD1 and SOD2 protein expression and bioactivity within CAR and ICAR. By the maintenance of adequate endometrium SOD1 and SOD2 activity, the conceptus limits lipid peroxidation during the peri-implantation period of pregnancy.

Original language	English
Pages (from-to)	77-83
Number of pages	7
Journal	Small Ruminant Research
Volume	141
Early online date	15 Jul 2016
DOIs	https://doi.org/10.1016/j.smallrumres.2016.07.006
Publication status	Published - Aug 2016

Keywords

Copper-zinc superoxide dismutase
Early pregnancy
Endometrium
Manganese superoxide dismutase
Sheep

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@article{03421d6ed399431480780011e9291a40,

title = "The conceptus induces a switch in protein expression and activities of superoxide dismutase 1 and 2 in the sheep endometrium during early pregnancy",

abstract = "There has been a growing interest in the importance of superoxide dismutases (SODs) in the regulation of endometrial function. However, little is known about endometrial SOD1 and SOD2 protein expression and activity associated with early conceptus development. We aimed to investigate changes in protein levels and activities of SOD1 and SOD2 in the sheep caruncular (CAR) and intercaruncular (ICAR) endometrium during the transition from pre-implantation (day 14) to implantation (day 16) and post-implantation (day 18) periods of pregnancy. Lipid peroxidation was assessed by measuring CAR and ICAR malondialdehyde (MDA) content. SOD1 activity increased from day 14 to day 18 (P <0.05) in CAR and from day 14 to day 18 (P <0.05) and from day 16 to day 18 (P <0.01) in ICAR. SOD1 protein level increased from day 16 to day 18 (P <0.05) in CAR and from days 14 to days 16 and 18 (P <0.05) in ICAR. SOD2 activity increased from day 16 to day 18 (P <0.05) in CAR and from days 14 and 16 to day 18 (P <0.001) in ICAR. SOD2 protein level increased from day 14 to day 18 (P <0.05) in CAR and from days 14 and 16 to day 18 (P <0.05) in ICAR. The content of MDA in CAR or ICAR did not alter significantly between stages of pregnancy. In conclusion, the early post-implanting conceptus co-ordinately up-regulates SOD1 and SOD2 protein expression and bioactivity within CAR and ICAR. By the maintenance of adequate endometrium SOD1 and SOD2 activity, the conceptus limits lipid peroxidation during the peri-implantation period of pregnancy.",

keywords = "Copper-zinc superoxide dismutase, Early pregnancy, Endometrium, Manganese superoxide dismutase, Sheep",

author = "Al-Gubory, {K. H.} and C. Garrel and N. Sugino and Fowler, {P. A.}",

note = "Acknowledgements We thank Philippe Bolifraud (INRA, France), Krawiec Angele, Sandra Grange, Laurence Puillet-Anselme (CHU Grenoble, France) and Margaret Fraser (Aberdeen, UK) for their expert technical assistance. The authors also thank the staff of the sheep sheds of Jouy-en-Josas (INRA, France). The authors would also like to thank the anonymous reviewers for their close examination of this article and their useful comments. Funding This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.",

year = "2016",

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doi = "10.1016/j.smallrumres.2016.07.006",

language = "English",

volume = "141",

pages = "77--83",

journal = "Small Ruminant Research",

issn = "0921-4488",

publisher = "Elsevier",

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TY - JOUR

T1 - The conceptus induces a switch in protein expression and activities of superoxide dismutase 1 and 2 in the sheep endometrium during early pregnancy

AU - Al-Gubory, K. H.

AU - Garrel, C.

AU - Sugino, N.

AU - Fowler, P. A.

N1 - Acknowledgements We thank Philippe Bolifraud (INRA, France), Krawiec Angele, Sandra Grange, Laurence Puillet-Anselme (CHU Grenoble, France) and Margaret Fraser (Aberdeen, UK) for their expert technical assistance. The authors also thank the staff of the sheep sheds of Jouy-en-Josas (INRA, France). The authors would also like to thank the anonymous reviewers for their close examination of this article and their useful comments. Funding This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.

PY - 2016/8

Y1 - 2016/8

N2 - There has been a growing interest in the importance of superoxide dismutases (SODs) in the regulation of endometrial function. However, little is known about endometrial SOD1 and SOD2 protein expression and activity associated with early conceptus development. We aimed to investigate changes in protein levels and activities of SOD1 and SOD2 in the sheep caruncular (CAR) and intercaruncular (ICAR) endometrium during the transition from pre-implantation (day 14) to implantation (day 16) and post-implantation (day 18) periods of pregnancy. Lipid peroxidation was assessed by measuring CAR and ICAR malondialdehyde (MDA) content. SOD1 activity increased from day 14 to day 18 (P <0.05) in CAR and from day 14 to day 18 (P <0.05) and from day 16 to day 18 (P <0.01) in ICAR. SOD1 protein level increased from day 16 to day 18 (P <0.05) in CAR and from days 14 to days 16 and 18 (P <0.05) in ICAR. SOD2 activity increased from day 16 to day 18 (P <0.05) in CAR and from days 14 and 16 to day 18 (P <0.001) in ICAR. SOD2 protein level increased from day 14 to day 18 (P <0.05) in CAR and from days 14 and 16 to day 18 (P <0.05) in ICAR. The content of MDA in CAR or ICAR did not alter significantly between stages of pregnancy. In conclusion, the early post-implanting conceptus co-ordinately up-regulates SOD1 and SOD2 protein expression and bioactivity within CAR and ICAR. By the maintenance of adequate endometrium SOD1 and SOD2 activity, the conceptus limits lipid peroxidation during the peri-implantation period of pregnancy.

AB - There has been a growing interest in the importance of superoxide dismutases (SODs) in the regulation of endometrial function. However, little is known about endometrial SOD1 and SOD2 protein expression and activity associated with early conceptus development. We aimed to investigate changes in protein levels and activities of SOD1 and SOD2 in the sheep caruncular (CAR) and intercaruncular (ICAR) endometrium during the transition from pre-implantation (day 14) to implantation (day 16) and post-implantation (day 18) periods of pregnancy. Lipid peroxidation was assessed by measuring CAR and ICAR malondialdehyde (MDA) content. SOD1 activity increased from day 14 to day 18 (P <0.05) in CAR and from day 14 to day 18 (P <0.05) and from day 16 to day 18 (P <0.01) in ICAR. SOD1 protein level increased from day 16 to day 18 (P <0.05) in CAR and from days 14 to days 16 and 18 (P <0.05) in ICAR. SOD2 activity increased from day 16 to day 18 (P <0.05) in CAR and from days 14 and 16 to day 18 (P <0.001) in ICAR. SOD2 protein level increased from day 14 to day 18 (P <0.05) in CAR and from days 14 and 16 to day 18 (P <0.05) in ICAR. The content of MDA in CAR or ICAR did not alter significantly between stages of pregnancy. In conclusion, the early post-implanting conceptus co-ordinately up-regulates SOD1 and SOD2 protein expression and bioactivity within CAR and ICAR. By the maintenance of adequate endometrium SOD1 and SOD2 activity, the conceptus limits lipid peroxidation during the peri-implantation period of pregnancy.

KW - Copper-zinc superoxide dismutase

KW - Early pregnancy

KW - Endometrium

KW - Manganese superoxide dismutase

KW - Sheep

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U2 - 10.1016/j.smallrumres.2016.07.006

DO - 10.1016/j.smallrumres.2016.07.006

M3 - Article

AN - SCOPUS:84978745554

VL - 141

SP - 77

EP - 83

JO - Small Ruminant Research

JF - Small Ruminant Research

SN - 0921-4488

ER -

The conceptus induces a switch in protein expression and activities of superoxide dismutase 1 and 2 in the sheep endometrium during early pregnancy

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