The core Planar Cell Polarity gene, Vangl2, maintains apical-basal organisation of the corneal epithelium

D. Alessio Panzica, Amy S. Findlay, Rianne Van Ladesteijn, J. Martin Collinson (Corresponding Author)

Research output: Contribution to journalArticle

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Abstract

The role of the core planar cell polarity (PCP) pathway protein, Vangl2, was investigated in the corneal epithelium of the mammalian eye, a paradigm anatomical model of planar cell migration. The gene was conditionally knocked out in vivo and knocked down by siRNA, followed by immunohistochemical, behavioural and morphological analysis of corneal epithelial cells. The primary defects observed in vivo were of apical-basal organisation of the corneal epithelium, with abnormal stratification throughout life, mislocalisation of the cell membrane protein, Scribble, to the basal side of cells, and partial loss of the epithelial basement membrane. Planar defects in migration after wounding and in presence of an applied electric field were noted. However, knockdown of Vangl2 also retarded cell migration in individual cells that had no contact with their neighbours, which precluded a classic PCP mechanism. It is concluded that some of the planar polarity phenotypes in PCP mutants may arise from disruption of apical-basal polarity.
Original languageEnglish
Pages (from-to)106-119
Number of pages14
JournalJournal of Anatomy
Volume234
Issue number1
Early online date17 Aug 2017
DOIs
Publication statusPublished - Jan 2019

Keywords

  • cornea
  • epithelium
  • planar cell polarity
  • Vangl2
  • apical-basal polarity

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