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Abstract
Chromosome ends in Saccharomyces cerevisiae are positioned in clusters at the nuclear rim. We report that Ctf18, Ctf8, and Dcc1, the subunits of a Replication Factor C (RFC)-like complex, are essential for the perinuclear positioning of telomeres. In both yeast and mammalian cells, peripheral nuclear positioning of chromatin during G1 phase correlates with late DNA replication. We find that the mislocalized telomeres of ctf18 cells still replicate late, showing that late DNA replication does not require peripheral positioning during G1. The Ku and Sir complexes have been shown to act through separate pathways to position telomeres, but in the absence of Ctf18 neither pathway can act fully to maintain telomere position. Surprisingly CTF18 is not required for Ku or Sir4-mediated peripheral tethering of a nontelomeric chromosome locus. Our results suggest that the Ctf18 RFC-like complex modifies telomeric chromatin to make it competent for normal localization to the nuclear periphery.
Original language | English |
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Pages (from-to) | 1505-1514 |
Number of pages | 9 |
Journal | EMBO Journal |
Volume | 25 |
Issue number | 7 |
DOIs | |
Publication status | Published - Apr 2006 |
Keywords
- Ctf8
- Dcc1
- Ku
- SIR proteins
- sister-chromatid cohesion
- cell nuclear antigen
- saccharomyces cerevisiae
- budding yeast
- genome stability
- chromosome ends
- binding protein
- SIR4 proteins
- DNA
- KU
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Dive into the research topics of 'The Ctf18 RFC-like complex positions yeast telomeres but does not specify their replication time'. Together they form a unique fingerprint.Activities
- 2 Attending/organising a Conference
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22nd International Conference on Yeast Genetics and Molecular Biology
Shinichiro Hiraga (Speaker)
2005Activity: Attending or organising an event › Attending/organising a Conference
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The 4th International Symposium on 3R; DNA replication, recombination and repair
Shinichiro Hiraga (Speaker)
2003Activity: Attending or organising an event › Attending/organising a Conference