The Ctf18 RFC-like complex positions yeast telomeres but does not specify their replication time

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Abstract

Chromosome ends in Saccharomyces cerevisiae are positioned in clusters at the nuclear rim. We report that Ctf18, Ctf8, and Dcc1, the subunits of a Replication Factor C (RFC)-like complex, are essential for the perinuclear positioning of telomeres. In both yeast and mammalian cells, peripheral nuclear positioning of chromatin during G1 phase correlates with late DNA replication. We find that the mislocalized telomeres of ctf18 cells still replicate late, showing that late DNA replication does not require peripheral positioning during G1. The Ku and Sir complexes have been shown to act through separate pathways to position telomeres, but in the absence of Ctf18 neither pathway can act fully to maintain telomere position. Surprisingly CTF18 is not required for Ku or Sir4-mediated peripheral tethering of a nontelomeric chromosome locus. Our results suggest that the Ctf18 RFC-like complex modifies telomeric chromatin to make it competent for normal localization to the nuclear periphery.

Original languageEnglish
Pages (from-to)1505-1514
Number of pages9
JournalEMBO Journal
Volume25
Issue number7
DOIs
Publication statusPublished - Apr 2006

Keywords

  • Ctf8
  • Dcc1
  • Ku
  • SIR proteins
  • sister-chromatid cohesion
  • cell nuclear antigen
  • saccharomyces cerevisiae
  • budding yeast
  • genome stability
  • chromosome ends
  • binding protein
  • SIR4 proteins
  • DNA
  • KU

Cite this

The Ctf18 RFC-like complex positions yeast telomeres but does not specify their replication time. / Hiraga, Shin-Ichiro; Robertson, E. D.; Donaldson, Anne Dunlop.

In: EMBO Journal, Vol. 25, No. 7, 04.2006, p. 1505-1514.

Research output: Contribution to journalArticle

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AB - Chromosome ends in Saccharomyces cerevisiae are positioned in clusters at the nuclear rim. We report that Ctf18, Ctf8, and Dcc1, the subunits of a Replication Factor C (RFC)-like complex, are essential for the perinuclear positioning of telomeres. In both yeast and mammalian cells, peripheral nuclear positioning of chromatin during G1 phase correlates with late DNA replication. We find that the mislocalized telomeres of ctf18 cells still replicate late, showing that late DNA replication does not require peripheral positioning during G1. The Ku and Sir complexes have been shown to act through separate pathways to position telomeres, but in the absence of Ctf18 neither pathway can act fully to maintain telomere position. Surprisingly CTF18 is not required for Ku or Sir4-mediated peripheral tethering of a nontelomeric chromosome locus. Our results suggest that the Ctf18 RFC-like complex modifies telomeric chromatin to make it competent for normal localization to the nuclear periphery.

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KW - genome stability

KW - chromosome ends

KW - binding protein

KW - SIR4 proteins

KW - DNA

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