The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction

Jesko Koehnke; Andrew F Bent; David Zollman; Kieran Smith; Wael E Houssen; Xiaofeng Zhu; Greg Mann; Tomas Lebl; Richard Scharff; Sally Shirran; Catherine H Botting; Marcel Jaspars; Ulrich Schwarz-Linek; James H Naismith

doi:10.1002/anie.201306302

The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction

Jesko Koehnke, Andrew F Bent, David Zollman, Kieran Smith, Wael E Houssen, Xiaofeng Zhu, Greg Mann, Tomas Lebl, Richard Scharff, Sally Shirran, Catherine H Botting, Marcel Jaspars, Ulrich Schwarz-Linek, James H Naismith

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Abstract

Counting backwards: The cyanobactin class of heterocyclases, exemplified by TruD, possess an almost unique combination of processivity, specificity, chemical versatility, and promiscuity. TruD is shown by biochemical assay to be an adenylase, and processes cysteines in a defined order. The entire substrate leader can be removed and TruD will process a single specific cysteine residue; however the role of leader is to permit processivity through a balance of recognition. ATP/AMP=adenosine tri/monophosphate; PPi=pyrophosphate.

Original language	English
Pages (from-to)	13991-13996
Number of pages	6
Journal	Angewandte Chemie International Edition
Volume	52
Issue number	52
Early online date	8 Nov 2013
DOIs	https://doi.org/10.1002/anie.201306302
Publication status	Published - 23 Dec 2013

Keywords

adenylation
cyanobactins
heterocyclase
ribosomal peptide pathways

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Koehnke, J., Bent, A. F., Zollman, D., Smith, K., Houssen, W. E., Zhu, X., Mann, G., Lebl, T., Scharff, R., Shirran, S., Botting, C. H., Jaspars, M., Schwarz-Linek, U., & Naismith, J. H. (2013). The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction. Angewandte Chemie International Edition, 52(52), 13991-13996. https://doi.org/10.1002/anie.201306302

Koehnke, J, Bent, AF, Zollman, D, Smith, K, Houssen, WE, Zhu, X, Mann, G, Lebl, T, Scharff, R, Shirran, S, Botting, CH, Jaspars, M, Schwarz-Linek, U & Naismith, JH 2013, 'The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction', Angewandte Chemie International Edition, vol. 52, no. 52, pp. 13991-13996. https://doi.org/10.1002/anie.201306302

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title = "The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction",

abstract = "Counting backwards: The cyanobactin class of heterocyclases, exemplified by TruD, possess an almost unique combination of processivity, specificity, chemical versatility, and promiscuity. TruD is shown by biochemical assay to be an adenylase, and processes cysteines in a defined order. The entire substrate leader can be removed and TruD will process a single specific cysteine residue; however the role of leader is to permit processivity through a balance of recognition. ATP/AMP=adenosine tri/monophosphate; PPi=pyrophosphate.",

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AU - Zollman, David

AU - Smith, Kieran

AU - Houssen, Wael E

AU - Zhu, Xiaofeng

AU - Mann, Greg

AU - Lebl, Tomas

AU - Scharff, Richard

AU - Shirran, Sally

AU - Botting, Catherine H

AU - Jaspars, Marcel

AU - Schwarz-Linek, Ulrich

AU - Naismith, James H

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PY - 2013/12/23

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AB - Counting backwards: The cyanobactin class of heterocyclases, exemplified by TruD, possess an almost unique combination of processivity, specificity, chemical versatility, and promiscuity. TruD is shown by biochemical assay to be an adenylase, and processes cysteines in a defined order. The entire substrate leader can be removed and TruD will process a single specific cysteine residue; however the role of leader is to permit processivity through a balance of recognition. ATP/AMP=adenosine tri/monophosphate; PPi=pyrophosphate.

KW - adenylation

KW - cyanobactins

KW - heterocyclase

KW - ribosomal peptide pathways

U2 - 10.1002/anie.201306302

DO - 10.1002/anie.201306302

M3 - Article

C2 - 24214017

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JF - Angewandte Chemie International Edition

SN - 1433-7851

IS - 52

ER -

The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction

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